Package: COTAN Type: Package Title: COexpression Tables ANalysis Version: 0.1.0 Author: Silvia Giulia Galfrè, Francesco Morandin, Marco Pietrosanto, Federico Cremisi, Manuela Helmer-Citterich Maintainer: Silvia Giulia Galfrè <[email protected]> Description: COTAN is a statistical and computational method to analyze the co-expression of gene pairs at single cell level. It provides the foundation for single-cell gene interactome analysis. The basic idea is studying the zero UMI counts’ distribution instead of focusing on positive counts; this is done with a generalized contingency tables framework. COTAN can effectively assess the correlated or anti-correlated expression of gene pairs. It provides a numerical index related to the correlation and an approximate p-value for the associated independence test. COTAN can also evaluate whether single genes are differentially expressed, scoring them with a newly defined global differentiation index. Moreover, this approach provides ways to plot and cluster genes according to their co-expression pattern with other genes, effectively helping the study of gene interactions and becoming a new tool to identify cell-identity marker genes. Depends: R (>= 3.5.0) License: CC-BY-ND 4.0 Encoding: UTF-8 RoxygenNote: 7.1.1 Imports: dplyr, Matrix, ggplot2, ggrepel, data.table, parallel, tibble, dplyr, tidyr, basilisk, reticulate, ComplexHeatmap Suggests: testthat (>= 3.0.0), spelling, knitr, rmarkdown, data.table, R.utils, Matrix, dendextend, Rtsne, plotly, tidyverse, htmlwidgets, MASS Config/testthat/edition: 3 SystemRequirements: python Language: en-US VignetteBuilder: knitr