% Generated by roxygen2: do not edit by hand % Please edit documentation in R/TENxFileList-class.R \name{TENxFileList} \alias{TENxFileList} \title{TENxFileList: Represent groups of files from 10X Genomic} \usage{ TENxFileList(..., version, compressed = FALSE) } \arguments{ \item{...}{Typically, a file path to a tarball archive. Can be named arguments corresponding to file paths, or a named list of file paths.} \item{version}{character(1) The version in the tarball. See details.} \item{compressed}{logical(1) Whether or not the file provided is compressed, usually as \code{tar.gz} (default FALSE)} } \value{ Either a \code{SingleCellExperiment} or a list of imported data } \description{ This constructor function is meant to handle \code{.tar.gz} tarball files from 10X Genomics. } \details{ These tarballs usually contain three files: \enumerate{ \item \code{matrix.mtx.gz} - the counts matrix \item \code{features.tsv.gz} - row metadata usually represented as \code{rowData} \item \code{barcodes.tsv.gz} - column names corresponding to cell barcode identifiers If all above files are in the tarball, the import method will provide a \code{SingleCellExperiment}. Otherwise, a simple list of imported data is given. Note that version "3" uses 'features.tsv.gz' and version "2" uses 'genes.tsv.gz'. If known, indicate the \code{version} argument in the \code{TENxFileList} constructor function. } } \examples{ fl <- system.file( "extdata", "pbmc_granulocyte_sorted_3k_ff_bc_ex_matrix.tar.gz", package = "TENxIO", mustWork = TRUE ) ## Method 1 (tarball) TENxFileList(fl) ## metadata before import metadata(TENxFileList(fl)) ## import() method import(TENxFileList(fl)) ## metadata after import import(TENxFileList(fl)) |> metadata() ## untar to simulate folder output dir.create(tdir <- tempfile()) untar(fl, exdir = tdir) ## Method 2 (folder) TENxFileList(tdir) import(TENxFileList(tdir)) ## Method 3 (list of TENxFile objects) files <- list.files(tdir, recursive = TRUE, full.names = TRUE) names(files) <- basename(files) filelist <- lapply(files, TENxFile) TENxFileList(filelist, compressed = FALSE) ## Method 4 (SimpleList) TENxFileList(as(filelist, "SimpleList"), compressed = FALSE) ## Method 5 (named arguments) TENxFileList( barcodes.tsv.gz = TENxFile(files[1]), features.tsv.gz = TENxFile(files[2]), matrix.mtx.gz = TENxFile(files[3]) ) unlink(tdir, recursive = TRUE) }