% Generated by roxygen2: do not edit by hand % Please edit documentation in R/opsDesign.R \name{getBarcodeDistanceMatrix} \alias{getBarcodeDistanceMatrix} \title{Get distance between query and target sets of barcodes} \usage{ getBarcodeDistanceMatrix( queryBarcodes, targetBarcodes = NULL, binnarize = TRUE, min_dist_edit = NULL, dist_method = c("hamming", "levenshtein"), ignore_diagonal = TRUE, splitByChunks = FALSE, n_chunks = NULL ) } \arguments{ \item{queryBarcodes}{Character vector of DNA sequences or DNAStringSet.} \item{targetBarcodes}{Optional character vector of DNA sequences or DNAStringSet. If NULL, distances will be calculated between barcodes provided in \code{queryBarcodes}.} \item{binnarize}{Should the distance matrix be made binnary? TRUE by default. See details section.} \item{min_dist_edit}{Integer specifying the minimum distance edit required for barcodes to be considered dissimilar when \code{binnarize=TRUE}, ignored otherwise.} \item{dist_method}{String specifying distance method. Must be either "hamming" (default) or "levenshtein".} \item{ignore_diagonal}{When \code{targetBarcodes=NULL}, should the diagonal distances be set to 0 to ignore self distances? TRUE by default.} \item{splitByChunks}{Should distances be calculated in a chunk-wise manner? FALSE by default. Highly recommended when the set of query barcodes is large to reduce memory footprint.} \item{n_chunks}{Integer specifying the number of chunks to be used when \code{splitByChunks=TRUE}. If NULL (default), number of chunks will be chosen automatically.} } \value{ A sparse matrix of class \code{dgCMatrix} or \code{dsCMatrix} in which rows correspond to \code{queryBarcodes} and columns correspond to \code{targetBarcodes}. If \code{binnarize=TRUE}, a value of 0 indicates that two barcodes have a distance greater of equal to \code{min_dist_edit}, otherwise the value is 1. If If \code{binnarize=FALSE}, values represent the actual calculated distances between barcodes. } \description{ Get distance between query and target sets of barcodes } \examples{ data(guideSetExample, package="crisprDesign") guideSetExample <- addOpsBarcodes(guideSetExample) barcodes <- as.character(guideSetExample$opsBarcode) dist <- getBarcodeDistanceMatrix(barcodes, min_dist_edit=2) } \author{ Jean-Philippe Fortin }