man/runBarcodeRankDrops.Rd
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 % Generated by roxygen2: do not edit by hand
 % Please edit documentation in R/dropletUtils_barcodeRank.R
 \name{runBarcodeRankDrops}
 \alias{runBarcodeRankDrops}
 \title{Identify empty droplets using \link[DropletUtils]{barcodeRanks}.}
 \usage{
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 runBarcodeRankDrops(
   inSCE,
   sample = NULL,
   useAssay = "counts",
   lower = 100,
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   fitBounds = NULL,
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   df = 20
 )
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 }
 \arguments{
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 \item{inSCE}{A \linkS4class{SingleCellExperiment} object. Must contain a raw 
 counts matrix before empty droplets have been removed.}
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 \item{sample}{Character vector or colData variable name. Indicates which 
 sample each cell belongs to. Default \code{NULL}.}
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 \item{useAssay}{A string specifying which assay in the SCE to use. Default 
 \code{"counts"}}
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 \item{lower}{See \link[DropletUtils]{barcodeRanks} for more information. 
 Default \code{100}.}
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 \item{fitBounds}{See \link[DropletUtils]{barcodeRanks} for more information. 
 Default \code{NULL}.}
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 \item{df}{See \link[DropletUtils]{barcodeRanks} for more information. Default 
 \code{20}.}
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 }
 \value{
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 A \linkS4class{SingleCellExperiment} object with the
 \link[DropletUtils]{barcodeRanks} output table appended to the
 \link{colData} slot. The columns include
 \code{dropletUtils_BarcodeRank_Knee} and 
 \code{dropletUtils_barcodeRank_inflection}. Please refer to the documentation
 of \link[DropletUtils]{barcodeRanks} for details.
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 }
 \description{
 Run \link[DropletUtils]{barcodeRanks} on a count matrix
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 provided in a \linkS4class{SingleCellExperiment} object. Distinguish between 
 droplets containing cells and ambient RNA in a droplet-based single-cell RNA 
 sequencing experiment.
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 }
 \examples{
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 data(scExample, package = "singleCellTK")
 sce <- runBarcodeRankDrops(inSCE = sce)
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 }
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 \seealso{
 \code{\link[DropletUtils]{barcodeRanks}}, 
 \code{\link{runDropletQC}}, \code{\link{plotBarcodeRankDropsResults}}
 }