% Generated by roxygen2: do not edit by hand % Please edit documentation in R/htmlReports.R \name{reportSeuratRun} \alias{reportSeuratRun} \title{Generates an HTML report for Seurat Run (including Normalization, Feature Selection, Dimensionality Reduction & Clustering) and returns the SCE object with the results computed and stored inside the object.} \usage{ reportSeuratRun( inSCE, biological.group = NULL, phenotype.groups = NULL, variable.features = 2000, pc.count = 50, runHVG = TRUE, plotHVG = TRUE, runDimRed = TRUE, plotJackStraw = FALSE, plotElbowPlot = TRUE, plotHeatmaps = TRUE, runClustering = TRUE, plotTSNE = TRUE, plotUMAP = TRUE, minResolution = 0.3, maxResolution = 1.5, outputFile = NULL, outputPath = NULL, subtitle = NULL, authors = NULL, showSession = FALSE, pdf = FALSE, forceRun = TRUE ) } \arguments{ \item{inSCE}{Input \code{\link[SingleCellExperiment]{SingleCellExperiment}} object.} \item{biological.group}{A character value that specifies the name of the \code{colData()} column to use as the main biological group in the Seurat report for tSNE & UMAP visualization.} \item{phenotype.groups}{A character value that specifies the name of the \code{colData()} column to use as additional phenotype variables in the Seurat report for tSNE & UMAP visualization.} \item{variable.features}{A numeric value indicating the number of top variable genes to identify in the report. Default is \code{2000}.} \item{pc.count}{A numeric value indicating the number of principal components to use in the analysis workflow. Default is \code{50}.} \item{runHVG}{A logical value indicating if feature selection should be run in the report. Default \code{TRUE}.} \item{plotHVG}{A logical value indicating if the top variable genes should be visualized through a mean-to-variance plot. Default is \code{TRUE}.} \item{runDimRed}{A logical value indicating if PCA should be computed in the report. Default is \code{TRUE}.} \item{plotJackStraw}{A logical value indicating if the JackStraw plot should be visualized for the principal components. Default is \code{FALSE}.} \item{plotElbowPlot}{A logical value indicating if the ElbowPlot should be visualized for the principal components. Default is \code{FALSE}.} \item{plotHeatmaps}{A logical value indicating if the Heatmaps should be visualized for the principal components. Default is \code{FALSE}.} \item{runClustering}{A logical value indicating if Clustering should be run over multiple resolutions as defined by the \code{minResolution} and \code{maxResolution} parameters. Default is \code{TRUE}.} \item{plotTSNE}{A logical value indicating if TSNE plot should be visualized for clusters. Default is \code{TRUE}.} \item{plotUMAP}{A logical value indicating if UMAP plot should be visualized for clusters. Default is \code{TRUE}.} \item{minResolution}{A numeric value indicating the minimum resolution to use for clustering. Default \code{0.3}.} \item{maxResolution}{A numeric value indicating the maximum resolution to use for clustering. Default \code{1.5}.} \item{outputFile}{Specify the name of the generated output HTML file. If \code{NULL} then the output file name will be based on the name of the Rmarkdown template. Default \code{NULL}.} \item{outputPath}{Specify the name of the output directory to save the rendered HTML file. If \code{NULL} the file is stored to the current working directory. Default \code{NULL}.} \item{subtitle}{A character value specifying the subtitle to use in the report. Default \code{NULL}.} \item{authors}{A character value specifying the names of the authors to use in the report. Default \code{NULL}.} \item{showSession}{A logical value indicating if session information should be displayed or not. Default is \code{FALSE}.} \item{pdf}{A logical value indicating if a pdf should also be generated for each figure in the report. Default is \code{FALSE}.} \item{forceRun}{A logical value indicating if all computations previously computed should be re-calculated regardless if these computations are available in the input object. Default is \code{TRUE}.} } \value{ A \code{\link[SingleCellExperiment]{SingleCellExperiment}} object with computations stored. } \description{ Generates an HTML report for Seurat Run (including Normalization, Feature Selection, Dimensionality Reduction & Clustering) and returns the SCE object with the results computed and stored inside the object. }