% Generated by roxygen2: do not edit by hand % Please edit documentation in R/doubletFinder_doubletDetection.R \name{runDoubletFinder} \alias{runDoubletFinder} \title{Generates a doublet score for each cell via doubletFinder} \usage{ runDoubletFinder( inSCE, sample = NULL, useAssay = "counts", seed = 12345, seuratNfeatures = 2000, seuratPcs = seq(15), seuratRes = 1.5, sct = FALSE, formationRate = 0.075, nCores = NULL, verbose = FALSE ) } \arguments{ \item{inSCE}{inSCE A \linkS4class{SingleCellExperiment} object.} \item{sample}{Character vector or colData variable name. Indicates which sample each cell belongs to. Default \code{NULL}.} \item{useAssay}{A string specifying which assay in the SCE to use. Default \code{"counts"}.} \item{seed}{Seed for the random number generator, can be set to \code{NULL}. Default \code{12345}.} \item{seuratNfeatures}{Integer. Number of highly variable genes to use. Default \code{2000}.} \item{seuratPcs}{Numeric vector. The PCs used in seurat function to determine number of clusters. Default \code{1:15}.} \item{seuratRes}{Numeric vector. The resolution parameter used in Seurat, which adjusts the number of clusters determined via the algorithm. Default \code{1.5}.} \item{sct}{Whether or not to use SCT. Default \code{FALSE}.} \item{formationRate}{Doublet formation rate used within algorithm. Default \code{0.075}.} \item{nCores}{Number of cores used for running the function. Default \code{NULL}.} \item{verbose}{Boolean. Wheter to print messages from Seurat and DoubletFinder. Default \code{FALSE}.} } \value{ \linkS4class{SingleCellExperiment} object containing the \code{doublet_finder_doublet_score} variable in \code{colData} slot. } \description{ Uses doubletFinder to determine cells within the dataset suspected to be doublets. } \examples{ data(scExample, package = "singleCellTK") options(future.globals.maxSize = 786432000) sce <- subsetSCECols(sce, colData = "type != 'EmptyDroplet'") sce <- runDoubletFinder(sce) } \seealso{ \code{\link{runCellQC}}, \code{\link{plotDoubletFinderResults}} }