% Generated by roxygen2: do not edit by hand % Please edit documentation in R/enrichRSCE.R \name{runEnrichR} \alias{runEnrichR} \title{Run EnrichR on SCE object} \usage{ runEnrichR( inSCE, features, analysisName, db = NULL, by = "rownames", featureName = NULL ) } \arguments{ \item{inSCE}{A \linkS4class{SingleCellExperiment} object.} \item{features}{Character vector, selected genes for enrichment analysis.} \item{analysisName}{A string that identifies each specific analysis.} \item{db}{Character vector. Selected database name(s) from the enrichR database list. If \code{NULL} then EnrichR will be run on all the available databases on the enrichR database. See details. Default \code{NULL}} \item{by}{Character. From where should we find the \code{features}? \code{"rownames"} for from \code{rownames(inSCE)}, otherwise, from a column of feature metadata (\code{rowData(inSCE)[[by]]}). See details. Default \code{"rownames"}.} \item{featureName}{Character. Indicates the actual feature identifiers to be passed to EnrichR. Can be \code{"rownames"}, a column in feature metadata (\code{rowData(inSCE)[[featureName]]}), or a character vector with its length equals to \code{nrow(inSCE)}. See details. Default \code{"rownames"}.} } \value{ Updates \code{inSCE} metadata with a data.frame of enrichment terms overlapping in the respective databases along with p-values, z-scores etc. } \description{ Run EnrichR on SCE object } \details{ EnrichR works by querying the specified \code{features} to its online databases, thus it requires the Internet connection. Available \code{db} options could be shown by running \code{enrichR::listEnrichrDbs()$libraryName} This function checks for the existence of features in the SCE object. When \code{features} do not have a match in \code{rownames(inSCE)}, users may try to specify \code{by} to pass the check. EnrichR expects gene symbols/names as the input (i.e. Ensembl ID might not work). When specified \code{features} are not qualified for this, users may try to specify \code{featureName} to change the identifier type to pass to EnrichR. } \examples{ data("mouseBrainSubsetSCE") if (Biobase::testBioCConnection()) { mouseBrainSubsetSCE <- runEnrichR(mouseBrainSubsetSCE, features = c("Vamp7","Cntn2","Olig1"), db = "GO_Cellular_Component_2025", analysisName = "analysis1") } } \seealso{ \code{\link{getEnrichRResult}} }