% Generated by roxygen2: do not edit by hand % Please edit documentation in R/runTSCAN.R \name{runTSCANDEG} \alias{runTSCANDEG} \title{Test gene expression changes along a TSCAN trajectory path} \usage{ runTSCANDEG(inSCE, pathIndex, useAssay = "logcounts", discardCluster = NULL) } \arguments{ \item{inSCE}{Input \linkS4class{SingleCellExperiment} object.} \item{pathIndex}{Path index for which the pseudotime values should be used. This corresponds to the terminal node of specific path from the root node to the terminal node. Run \code{listTSCANTerminalNodes(inSCE)} for available options.} \item{useAssay}{Character. The name of the assay to use for testing the expression change. Should be log-normalized. Default \code{"logcounts"}} \item{discardCluster}{Cluster(s) which are not of use or masks other interesting effects can be discarded. Default \code{NULL}.} } \value{ The input \code{inSCE} with results updated in \code{metadata}. } \description{ Wrapper for identifying genes with significant changes with respect to one of the TSCAN pseudotime paths } \examples{ data("mouseBrainSubsetSCE", package = "singleCellTK") mouseBrainSubsetSCE <- runTSCAN(inSCE = mouseBrainSubsetSCE, useReducedDim = "PCA_logcounts") terminalNodes <- listTSCANTerminalNodes(mouseBrainSubsetSCE) mouseBrainSubsetSCE <- runTSCANDEG(inSCE = mouseBrainSubsetSCE, pathIndex = terminalNodes[1]) } \author{ Nida Pervaiz }