Browse code

Seurat workflow updated

Nida Pervaiz authored on 31/12/2023 11:42:44
Showing 1 changed files
... ...
@@ -43,5 +43,6 @@ sce <- runSeuratNormalizeData(sce, useAssay = "counts")
43 43
 sce <- runSeuratFindHVG(sce, useAssay = "counts")
44 44
 sce <- runSeuratScaleData(sce, useAssay = "counts")
45 45
 sce <- runSeuratPCA(sce, useAssay = "counts")
46
-plotSeuratReduction(sce, useReductionPlot = "pca")}
46
+plotSeuratReduction(sce, useReduction = "pca")
47
+}
47 48
 }
Browse code

Renamed seurat methods

Irzam Sarfraz authored on 05/04/2022 09:46:47
Showing 1 changed files
1 1
new file mode 100644
... ...
@@ -0,0 +1,47 @@
1
+% Generated by roxygen2: do not edit by hand
2
+% Please edit documentation in R/seuratFunctions.R
3
+\name{plotSeuratReduction}
4
+\alias{plotSeuratReduction}
5
+\title{plotSeuratReduction
6
+Plots the selected dimensionality reduction method}
7
+\usage{
8
+plotSeuratReduction(
9
+  inSCE,
10
+  useReduction = c("pca", "ica", "tsne", "umap"),
11
+  showLegend = FALSE,
12
+  groupBy = NULL,
13
+  splitBy = NULL
14
+)
15
+}
16
+\arguments{
17
+\item{inSCE}{(sce) object which has the selected dimensionality reduction
18
+algorithm already computed and stored}
19
+
20
+\item{useReduction}{Dimentionality reduction to plot. One of "pca", "ica",
21
+"tsne", or "umap". Default \code{"umap"}.}
22
+
23
+\item{showLegend}{Select if legends and labels should be shown on the output
24
+plot or not. Either "TRUE" or "FALSE". Default \code{FALSE}.}
25
+
26
+\item{groupBy}{Specify a colData column name that be used for grouping.
27
+Default is \code{NULL}.}
28
+
29
+\item{splitBy}{Specify a colData column name that be used for splitting the
30
+output plot. Default is \code{NULL}.}
31
+}
32
+\value{
33
+plot object
34
+}
35
+\description{
36
+plotSeuratReduction
37
+Plots the selected dimensionality reduction method
38
+}
39
+\examples{
40
+data(scExample, package = "singleCellTK")
41
+\dontrun{
42
+sce <- runSeuratNormalizeData(sce, useAssay = "counts")
43
+sce <- runSeuratFindHVG(sce, useAssay = "counts")
44
+sce <- runSeuratScaleData(sce, useAssay = "counts")
45
+sce <- runSeuratPCA(sce, useAssay = "counts")
46
+plotSeuratReduction(sce, useReductionPlot = "pca")}
47
+}