Research Article
BibTex RIS Cite

Year 2025, Volume: 10 Issue: 2, 115 - 121, 31.08.2025

Murat Can Demir , Cihan Kaçar , Yavuz Öztürkler , Semra Kaya , Mushap Kuru , Uğur Kara , Hasan Mutlu , Savaş Yıldız , Taygun Gökdemir , Muhammet Ali Karadağ , Merve Sena Demir , Türkan Gümüşay

https://doi.org/10.31797/vetbio.1686254

Abstract

Project Number

2022-TS-78

References

  • Akçapınar, H. (1994). Koyun Yetiştiriciliği. Medisan Yayınevi.
  • Al Yacoub AN, Gauly M, Sohnrey B, & Holtz W. (2011). Fixed-time deep uterine insemination in PGF2α-synchronized goats. Theriogenology, 76(9), 1730–1735. https://doi.org/10.1016/j.theriogenology.2011.07.005
  • Alkan, K. karakaş. (2021). Koyun ve Keçilerde Embriyo Üretimi ve Transferi. Turkiye Klinikleri Veterinary Sciences-Obstetrics and Gynecology-Special Topics , 7(3), 50–55.
  • Alkan, K., Özkan M, & Kaymaz M. (2017). Küçük Ruminantlarda Yardımcı Üreme Teknolojilerinin Kullanım Seçenekleri. Turkiye Klinikleri Veterinary Sciences-Obstetrics and Gynecology, 3(2), 113–122.
  • Amiridis, G. S., & Cseh, S. (2012). Assisted reproductive technologies in the reproductive management of small ruminants. Animal Reproduction Science, 130(3–4), 152–161. https://doi.org/10.1016/j.anireprosci.2012.01.009
  • Azawi, O. I., & Al-Mola, M. K. M. A. (2010). A study on superovulation using FSH and eCG in Awassi ewes. Tropical Animal Health and Production, 42(5), 799–801. https://doi.org/10.1007/S11250-009-9489-2
  • Blanco, M. R., Simonetti, L., & Rivera, O. E. (2003). Embryo production and progesterone profiles in ewes superovulated with different hormonal treatments. Small Ruminant Research, 47(3), 183–191. https://doi.org/10.1016/S0921-4488(02)00245-6
  • Chagas e Silva, J., Lopes da Costa, L., Cidadão, R., & Robalo Silva, J. (2003). Plasma progesterone profiles, ovulation rate, donor embryo yield and recipient embryo survival in native Saloia sheep in the fall and spring breeding seasons. Theriogenology, 60(3), 521–532. https://doi.org/10.1016/S0093-691X(03)00045-1
  • Cordeiro, M. F., Lima-Verde, J. B., Lopes-Júnior, E. S., Teixeira, D. I. A., Farias, L. N., Salles, H. O., Simplı́cio, A. A., Rondina, D., & Freitas, V. J. F. (2003). Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH. Small Ruminant Research, 49(1), 19–23. https://doi.org/10.1016/S0921-4488(03)00059-2
  • Cuadro, F., dos Santos-Neto, P. C., Pinczak, A., Barrera, N., Crispo, M., & Menchaca, A. (2018). Serum progesterone concentrations during FSH superstimulation of the first follicular wave affect embryo production in sheep. Animal Reproduction Science, 196, 205–210. https://doi.org/10.1016/j.anireprosci.2018.08.011
  • Flores-Foxworth, G., McBride, B. M., Kraemer, D. C., & Nuti, L. C. (1992). A comparison between laparoscopic and transcervical embryo collection and transfer in goats. Theriogenology, 37(1), 213. https://doi.org/10.1016/0093-691X(92)90282-V
  • Gonzalez-Bulnes, A., Garcia-Garcia, R. M., Santiago-Moreno, J., Dominguez, V., Lopez-Sebastian, A., & Cocero, M. J. (2003). Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes. Theriogenology, 60(2), 281–288. https://doi.org/10.1016/s0093-691x(02)01367-5
  • Karaoğlu, M., Macit, M., & Emsen, H. (2001). Tuj kuzularının büyüme ve gelişme özellikleri ile yaşama gücü üzerine bir araştırma. Turkish Journal of Veterinary and Animal Sciences, 25(3), 261–266.
  • Kayaalp, E. (2010). Akkaraman ırkı koyunlara FSH uygulaması sonucu elde edilen süperovulasyon cevabının değerlendirilmesi. Selçuk Üniversitesi.
  • Kershaw, C. M., Khalid, M., McGowan, M. R., Ingram, K., Leethongdee, S., Wax, G., & Scaramuzzi, R. J. (2005). The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology, 64(5), 1225–1235. https://doi.org/10.1016/j.theriogenology.2005.02.017
  • Kopuzlu, S., & Emsen, H. (2004). Tuj Koyunlarının Bazı Yapağı Özelliklerinin Değerlendirilmesi. 4. Ulusal Zootekni Kongresi, 1, 179–185.
  • Köse, A., Çizmeci, S., Tur, İ., of, M. G.-E. J., & 2012, undefined. (2012). Evaluation of superovulation responses in some domestic ewe breeds. Eurasian Journal of Veterinary Sciences, 28(4), 224–228.
  • Li, Q. Y., Guan, H., Hou, J., An, X. R., & Chen, Y. F. (2008). Technical note: Transfer of ovine embryos through a simplified mini-laparotomy technique1. Journal of Animal Science, 86(11), 3224–3227. https://doi.org/10.2527/jas.2008-0846
  • Lukanina, V. A., Chinarov, R. Yu., Pozyabin, S. V., Shumakov, N. I., Cherkasova, O. V., & Singina, G. N. (2024). Comparative Study of the Effectiveness of Laparotomic and Laparoscopic Methods of Transplantation of Cloned Embryos in Sheep. Russian Agricultural Sciences 2023 49:2, 49(2), S339–S344. https://doi.org/10.3103/S1068367423080128
  • Martemucci, G., & D’Alessandro, A. G. (2013). Efficiency of FSH/LH treatments for in vivo production of embryos and their cryopreservation by different methods in goats. Small Ruminant Research, 114(2–3), 264–271. https://doi.org/10.1016/j.smallrumres.2013.07.006
  • McKelvey, W. A. C., Robinson, J. J., Aitken, R. P., & Robertson, I. S. (1986). Repeated recoveries of embryos from ewes by laparoscopy. Theriogenology, 25(6), 855–865. https://doi.org/10.1016/0093-691X(86)90102-0
  • Mollo, M. R., Monteiro, P. L. J., Surjus, R. S., Martins, A. C., Ramos, A. F., Mourão, G. B., Carrijo, L. H. D., Lopes, G., Rumpf, R., Wiltbank, M. C., & Sartori, R. (2017). Embryo production in heifers with low or high dry matter intake submitted to superovulation. Theriogenology, 92, 30–35. https://doi.org/10.1016/j.theriogenology.2017.01.015
  • Mueller J. (1993). Utilización de la inseminación artificial y la superovulación con transferencia de embriones en el mejoramiento genético de ovinos. Comunicación Técnica Del INTA CD Producción Animal. https://scholar.google.com/scholar?hl=tr&as_sdt=0%2C5&q=Mueller%2C+J.+1993.+Utilizaci%C3%B3n+de+la+inseminaci%C3%B3n+artificial+y+la+superovulaci%C3%B3n+con+transferencia+de+embriones+en+el+mejoramiento+gen%C3%A9tico+de+ovinos.+Comunicaci%C3%B3n+T%C3%A9cnica+del+INTACD+Producci%C3%B3n+Animal.+323%3A+1-8.&btnG=
  • Perera, G. D. R. K., Pushpakumara, P. G. A., De Silva, L. N. A., & Alexander, P. A. B. D. (2008). Production of genetically superior goats through embryo transfer technology in Sri Lanka. Tropical Agricultural Research, 20, 177–184.
  • Rahman, A., Ramli, A., & Embong, W. W. (2008). A review of reproductive biotechnologies and their application in goat. Biotechnology, 7(2), 371–384.
  • Robinson, J. J., Wallace, J. M., & Aitken, R. P. (1989). Fertilization and ovum recovery rates in superovulated ewes following cervical insemination or laparoscopic intrauterine insemination at different times after progestagen withdrawal and in one or both uterine horns. Reproduction, 87(2), 771–782. https://doi.org/10.1530/jrf.0.0870771
  • Russel, A. (1984). Body condition scoring of sheep. In Practice, 6(3), 91–93. https://doi.org/10.1136/inpract.6.3.91
  • Shin, S. T., Jang, S. K., Yang, H. S., Lee, O. K., Shim, Y. H., Choi, W. Il, Lee, D. S., Lee, G. S., Cho, J. K., & Lee, Y. W. (2008). Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus). Journal of Veterinary Science, 9(1), 103. https://doi.org/10.4142/jvs.2008.9.1.103
  • Shipley, C. F. B. , Buckrell, B. C. , Mylne, M. J. A. , POLLARD, J. , & Hunton, J. R. (2007). Artificial insemination and embryo transfer in sheep. Current Therapy in Large Animal Theriogenology, 629–641. https://scholar.google.com.tr/scholar?hl=tr&as_sdt=0%2C5&q=Artificial+insemination+and+embryo+transfer+in+sheep&btnG=
  • Smith, C. (1986). Use of embryo transfer in genetic improvement of sheep. Animal Science, 42(1), 81–88. https://doi.org/10.1017/S000335610001775X
  • Tittel, A., Treutner, K. H., Titkova, S., Öttinger, A., & Schumpelick, V. (2001). New adhesion formation after laparoscopic and conventional adhesiolysis. Surgical Endoscopy, 15(1), 44–46. https://doi.org/10.1007/s004640000256
  • Torres, S., & Sevellec, C. (1987). Repeated superovulation and surgical recovery of embryos in the ewe. Reproduction Nutrition Développement, 27(4), 859–563. https://doi.org/10.1051/rnd:19870612
  • Ulusoy, H., & Kaymaz, M. (2009). Koyunlarda gebelik muayenesi. Vet. Hek. Derg., 80(1), 31–36.
  • Whyman, D., & Moore, R. W. (1980). Effects of PMSG and the prostaglandin F-2α analogue, cloprostenol, on superovulation, fertilization and egg transport in the ewe. Reproduction, 60(2), 267–272. https://doi.org/10.1530/JRF.0.0600267

Comparison of laparoscopic and laparotomic methods in embryo transfer in Tuj ewes

Year 2025, Volume: 10 Issue: 2, 115 - 121, 31.08.2025

Murat Can Demir , Cihan Kaçar , Yavuz Öztürkler , Semra Kaya , Mushap Kuru , Uğur Kara , Hasan Mutlu , Savaş Yıldız , Taygun Gökdemir , Muhammet Ali Karadağ , Merve Sena Demir , Türkan Gümüşay

https://doi.org/10.31797/vetbio.1686254

Abstract

The aim of this study was to transfer fresh embryos obtained through a superovulation protocol in Tuj ewes to recipient animals using either laparoscopic or laparotomic methods, and to evaluate the embryos’ viability and survival rates. For this purpose, 10 Tuj ewes were used as donors and 20 Tuj ewes as recipients. Intravaginal progesterone-impregnated sponges were placed in the donor ewes during the breeding season for 5 days. FSH was applied in decreasing doses starting from the 8th day, and estrus ewes were mated with fertile rams. On day 7 following mating, embryos produced in vivo were collected surgically and evaluated under the stereo microscope. Intravaginal sponges were placed in the recipient sheep for 9 days for synchronization, and embryo transfer was performed on day 18 by laparoscopic and laparotomic methods. While the pregnancy rate was observed with the laparotomic method was 33.3%, the pregnancy rate with the laparoscopic method was determined to be 80% (P>0.05). In the evaluation of the obtained embryos, 15 morulae, 1 early blastocyst, 4 blastocysts, and 1 expanded blastocyst were determined. As a result, embryos obtained from Tuj ewes through superovulation protocols were transferred to recipient ewes via laparoscopic and laparotomic methods, and pregnancy was achieved. In the future, with more comprehensive studies using different protocols, the number of embryos obtained from Tuj ewes and transfer methods can be improved. It was also determined that this method could be used to protect local genetic resources of Tuj ewes.

Keywords

Embryo transfer FSH Laparoscopic Laparotomic Ewes Tuj.

Ethical Statement

This study protocol was approved by the Kafkas University Local Ethics Committee for Animal Experiments (KAFKAS-HADYEK / 2022-05).

Supporting Institution

The research work was supported by Scientific Research Projects Coordinator ship of Kafkas University (Project code: 2022-TS-78).

Project Number

2022-TS-78

References

  • Akçapınar, H. (1994). Koyun Yetiştiriciliği. Medisan Yayınevi.
  • Al Yacoub AN, Gauly M, Sohnrey B, & Holtz W. (2011). Fixed-time deep uterine insemination in PGF2α-synchronized goats. Theriogenology, 76(9), 1730–1735. https://doi.org/10.1016/j.theriogenology.2011.07.005
  • Alkan, K. karakaş. (2021). Koyun ve Keçilerde Embriyo Üretimi ve Transferi. Turkiye Klinikleri Veterinary Sciences-Obstetrics and Gynecology-Special Topics , 7(3), 50–55.
  • Alkan, K., Özkan M, & Kaymaz M. (2017). Küçük Ruminantlarda Yardımcı Üreme Teknolojilerinin Kullanım Seçenekleri. Turkiye Klinikleri Veterinary Sciences-Obstetrics and Gynecology, 3(2), 113–122.
  • Amiridis, G. S., & Cseh, S. (2012). Assisted reproductive technologies in the reproductive management of small ruminants. Animal Reproduction Science, 130(3–4), 152–161. https://doi.org/10.1016/j.anireprosci.2012.01.009
  • Azawi, O. I., & Al-Mola, M. K. M. A. (2010). A study on superovulation using FSH and eCG in Awassi ewes. Tropical Animal Health and Production, 42(5), 799–801. https://doi.org/10.1007/S11250-009-9489-2
  • Blanco, M. R., Simonetti, L., & Rivera, O. E. (2003). Embryo production and progesterone profiles in ewes superovulated with different hormonal treatments. Small Ruminant Research, 47(3), 183–191. https://doi.org/10.1016/S0921-4488(02)00245-6
  • Chagas e Silva, J., Lopes da Costa, L., Cidadão, R., & Robalo Silva, J. (2003). Plasma progesterone profiles, ovulation rate, donor embryo yield and recipient embryo survival in native Saloia sheep in the fall and spring breeding seasons. Theriogenology, 60(3), 521–532. https://doi.org/10.1016/S0093-691X(03)00045-1
  • Cordeiro, M. F., Lima-Verde, J. B., Lopes-Júnior, E. S., Teixeira, D. I. A., Farias, L. N., Salles, H. O., Simplı́cio, A. A., Rondina, D., & Freitas, V. J. F. (2003). Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH. Small Ruminant Research, 49(1), 19–23. https://doi.org/10.1016/S0921-4488(03)00059-2
  • Cuadro, F., dos Santos-Neto, P. C., Pinczak, A., Barrera, N., Crispo, M., & Menchaca, A. (2018). Serum progesterone concentrations during FSH superstimulation of the first follicular wave affect embryo production in sheep. Animal Reproduction Science, 196, 205–210. https://doi.org/10.1016/j.anireprosci.2018.08.011
  • Flores-Foxworth, G., McBride, B. M., Kraemer, D. C., & Nuti, L. C. (1992). A comparison between laparoscopic and transcervical embryo collection and transfer in goats. Theriogenology, 37(1), 213. https://doi.org/10.1016/0093-691X(92)90282-V
  • Gonzalez-Bulnes, A., Garcia-Garcia, R. M., Santiago-Moreno, J., Dominguez, V., Lopez-Sebastian, A., & Cocero, M. J. (2003). Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes. Theriogenology, 60(2), 281–288. https://doi.org/10.1016/s0093-691x(02)01367-5
  • Karaoğlu, M., Macit, M., & Emsen, H. (2001). Tuj kuzularının büyüme ve gelişme özellikleri ile yaşama gücü üzerine bir araştırma. Turkish Journal of Veterinary and Animal Sciences, 25(3), 261–266.
  • Kayaalp, E. (2010). Akkaraman ırkı koyunlara FSH uygulaması sonucu elde edilen süperovulasyon cevabının değerlendirilmesi. Selçuk Üniversitesi.
  • Kershaw, C. M., Khalid, M., McGowan, M. R., Ingram, K., Leethongdee, S., Wax, G., & Scaramuzzi, R. J. (2005). The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology, 64(5), 1225–1235. https://doi.org/10.1016/j.theriogenology.2005.02.017
  • Kopuzlu, S., & Emsen, H. (2004). Tuj Koyunlarının Bazı Yapağı Özelliklerinin Değerlendirilmesi. 4. Ulusal Zootekni Kongresi, 1, 179–185.
  • Köse, A., Çizmeci, S., Tur, İ., of, M. G.-E. J., & 2012, undefined. (2012). Evaluation of superovulation responses in some domestic ewe breeds. Eurasian Journal of Veterinary Sciences, 28(4), 224–228.
  • Li, Q. Y., Guan, H., Hou, J., An, X. R., & Chen, Y. F. (2008). Technical note: Transfer of ovine embryos through a simplified mini-laparotomy technique1. Journal of Animal Science, 86(11), 3224–3227. https://doi.org/10.2527/jas.2008-0846
  • Lukanina, V. A., Chinarov, R. Yu., Pozyabin, S. V., Shumakov, N. I., Cherkasova, O. V., & Singina, G. N. (2024). Comparative Study of the Effectiveness of Laparotomic and Laparoscopic Methods of Transplantation of Cloned Embryos in Sheep. Russian Agricultural Sciences 2023 49:2, 49(2), S339–S344. https://doi.org/10.3103/S1068367423080128
  • Martemucci, G., & D’Alessandro, A. G. (2013). Efficiency of FSH/LH treatments for in vivo production of embryos and their cryopreservation by different methods in goats. Small Ruminant Research, 114(2–3), 264–271. https://doi.org/10.1016/j.smallrumres.2013.07.006
  • McKelvey, W. A. C., Robinson, J. J., Aitken, R. P., & Robertson, I. S. (1986). Repeated recoveries of embryos from ewes by laparoscopy. Theriogenology, 25(6), 855–865. https://doi.org/10.1016/0093-691X(86)90102-0
  • Mollo, M. R., Monteiro, P. L. J., Surjus, R. S., Martins, A. C., Ramos, A. F., Mourão, G. B., Carrijo, L. H. D., Lopes, G., Rumpf, R., Wiltbank, M. C., & Sartori, R. (2017). Embryo production in heifers with low or high dry matter intake submitted to superovulation. Theriogenology, 92, 30–35. https://doi.org/10.1016/j.theriogenology.2017.01.015
  • Mueller J. (1993). Utilización de la inseminación artificial y la superovulación con transferencia de embriones en el mejoramiento genético de ovinos. Comunicación Técnica Del INTA CD Producción Animal. https://scholar.google.com/scholar?hl=tr&as_sdt=0%2C5&q=Mueller%2C+J.+1993.+Utilizaci%C3%B3n+de+la+inseminaci%C3%B3n+artificial+y+la+superovulaci%C3%B3n+con+transferencia+de+embriones+en+el+mejoramiento+gen%C3%A9tico+de+ovinos.+Comunicaci%C3%B3n+T%C3%A9cnica+del+INTACD+Producci%C3%B3n+Animal.+323%3A+1-8.&btnG=
  • Perera, G. D. R. K., Pushpakumara, P. G. A., De Silva, L. N. A., & Alexander, P. A. B. D. (2008). Production of genetically superior goats through embryo transfer technology in Sri Lanka. Tropical Agricultural Research, 20, 177–184.
  • Rahman, A., Ramli, A., & Embong, W. W. (2008). A review of reproductive biotechnologies and their application in goat. Biotechnology, 7(2), 371–384.
  • Robinson, J. J., Wallace, J. M., & Aitken, R. P. (1989). Fertilization and ovum recovery rates in superovulated ewes following cervical insemination or laparoscopic intrauterine insemination at different times after progestagen withdrawal and in one or both uterine horns. Reproduction, 87(2), 771–782. https://doi.org/10.1530/jrf.0.0870771
  • Russel, A. (1984). Body condition scoring of sheep. In Practice, 6(3), 91–93. https://doi.org/10.1136/inpract.6.3.91
  • Shin, S. T., Jang, S. K., Yang, H. S., Lee, O. K., Shim, Y. H., Choi, W. Il, Lee, D. S., Lee, G. S., Cho, J. K., & Lee, Y. W. (2008). Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus). Journal of Veterinary Science, 9(1), 103. https://doi.org/10.4142/jvs.2008.9.1.103
  • Shipley, C. F. B. , Buckrell, B. C. , Mylne, M. J. A. , POLLARD, J. , & Hunton, J. R. (2007). Artificial insemination and embryo transfer in sheep. Current Therapy in Large Animal Theriogenology, 629–641. https://scholar.google.com.tr/scholar?hl=tr&as_sdt=0%2C5&q=Artificial+insemination+and+embryo+transfer+in+sheep&btnG=
  • Smith, C. (1986). Use of embryo transfer in genetic improvement of sheep. Animal Science, 42(1), 81–88. https://doi.org/10.1017/S000335610001775X
  • Tittel, A., Treutner, K. H., Titkova, S., Öttinger, A., & Schumpelick, V. (2001). New adhesion formation after laparoscopic and conventional adhesiolysis. Surgical Endoscopy, 15(1), 44–46. https://doi.org/10.1007/s004640000256
  • Torres, S., & Sevellec, C. (1987). Repeated superovulation and surgical recovery of embryos in the ewe. Reproduction Nutrition Développement, 27(4), 859–563. https://doi.org/10.1051/rnd:19870612
  • Ulusoy, H., & Kaymaz, M. (2009). Koyunlarda gebelik muayenesi. Vet. Hek. Derg., 80(1), 31–36.
  • Whyman, D., & Moore, R. W. (1980). Effects of PMSG and the prostaglandin F-2α analogue, cloprostenol, on superovulation, fertilization and egg transport in the ewe. Reproduction, 60(2), 267–272. https://doi.org/10.1530/JRF.0.0600267
There are 34 citations in total.

Details

Primary Language English
Subjects Veterinary Obstetrics and Gynecology
Journal Section Research Articles
Authors

Murat Can Demir 0000-0002-3803-069X

Cihan Kaçar 0000-0002-2642-697X

Yavuz Öztürkler 0000-0002-0681-9311

Semra Kaya 0000-0002-7520-6631

Mushap Kuru 0000-0003-4409-251X

Uğur Kara 0000-0002-7977-6826

Hasan Mutlu 0000-0001-7479-7057

Savaş Yıldız 0000-0002-1256-2428

Taygun Gökdemir 0000-0001-6049-7184

Muhammet Ali Karadağ 0000-0002-1791-9588

Merve Sena Demir 0000-0002-2630-8818

Türkan Gümüşay 0009-0001-5764-7417

Project Number 2022-TS-78
Publication Date August 31, 2025
Submission Date April 30, 2025
Acceptance Date June 30, 2025
Published in Issue Year 2025 Volume: 10 Issue: 2

Cite

APA Demir, M. C., Kaçar, C., Öztürkler, Y., … Kaya, S. (2025). Comparison of laparoscopic and laparotomic methods in embryo transfer in Tuj ewes. Journal of Advances in VetBio Science and Techniques, 10(2), 115-121. https://doi.org/10.31797/vetbio.1686254
 Download Cover Image

22563   CABI-Logo_NEW_accessible.jpg   logo_world_of_journals_no_margin.png  download download   download   download        download