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Subread
•
reset
5
votes
4
replies
1.3k
views
RSubread output significantly different from bash Subread output
Subread
Rsubread
updated 9 months ago by
ATpoint
★ 4.9k • written 9 months ago by
Aimee
▴ 10
0
votes
4
replies
1.1k
views
featureCounts error: Assertion `numOfBuckets > 0' failed
featureCounts
Subread
Bioconductor
14 months ago • updated 13 months ago
Jessica
• 0
4
votes
2
replies
1.8k
views
Behaviour of featureCounts when quantifying multi-mapping reads
Bioconductor
featurecounts
subread
15 months ago
Rachael
• 0
0
votes
0
replies
1.1k
views
Subread2.0.3 featureCounts segmentation fault when loading gtf file
Subread
GTF
featureCounts
segmentationfault
2.6 years ago
yao hua
• 0
0
votes
1
reply
1.4k
views
Subread installation on Linux ARM64
subread
featureCounts
updated 2.6 years ago by
Yang Liao
▴ 450 • written 2.6 years ago by
Keyu
• 0
1
vote
1
reply
3.6k
views
Quantification of Genes with RSubread::featureCounts() at exon-level vs gene-level?
Subread
RNASeq
featureCounts
quantification
updated 2.8 years ago by
Gordon Smyth
53k • written 2.8 years ago by
Sara
• 0
0
votes
0
replies
947
views
Option request for featureCounts: Add an order of read manipulation to 'shift > reduction > extension'
SubRead
featureCounts
updated 2.8 years ago by
ATpoint
★ 4.9k • written 2.8 years ago by
Leon
• 0
2
votes
2
replies
1.2k
views
featureCounts: No effect of setting -d and -D?
subread
featureCounts
2.9 years ago
dario.beraldi
• 0
0
votes
1
reply
5.1k
views
gff3 file for featureCounts
gtf/gff3
subread
featureCounts
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
selmanurkeskin
• 0
0
votes
1
reply
1.3k
views
DEXSeq Exon count not working, please help with an alternative like featureCounts
splicing
Exon
featureCounts
subread
DEXSeq
3.3 years ago
NIK
• 0
3
votes
11
replies
5.5k
views
The low successful assignment ratio of FeatureCounts
FeatureCounts
Rsubread
Subread
3.4 years ago
Sarah_piggy
▴ 10
1
vote
1
reply
1.1k
views
Threads on Subread
Slurm
Subread
updated 3.4 years ago by
ATpoint
★ 4.9k • written 3.4 years ago by
sha-ked
• 0
1
vote
4
replies
2.8k
views
Subread installation
subread
featureCounts
3.4 years ago
Lucy
▴ 60
0
votes
0
replies
1.1k
views
SUBREAD Aligning reads with reference genome - Error in align The number of input file names is different from the number of output file names.
align
Alignment
subread
3.4 years ago
saira
• 0
1
vote
5
replies
3.3k
views
featureCounts parameter queries - minOverlap, fracOverlap, fracOverlapFeature
subread
Rsubread
featureCounts
RNA-seq
3.6 years ago
Lucy
▴ 60
4
votes
4
replies
6.6k
views
FeatureCounts
counts
subread
RNASeq
featurecounts
3.8 years ago
k___
▴ 30
1
vote
1
reply
1.8k
views
Make featureCounts ignore soft clip and insertions when for calculating read overlap
subread
featureCounts
updated 4.1 years ago by
Wei Shi
★ 3.6k • written 4.1 years ago by
dario.beraldi
• 0
1
vote
5
replies
7.2k
views
featureCounts (subread v2.0.2) ERROR: invalid parameter: '−−countReadPairs'
subread
featureCounts
updated 2.1 years ago by
Yang Liao
▴ 450 • written 4.2 years ago by
s.muroy
▴ 10
2
votes
2
replies
2.4k
views
Error when loading annotation featureCounts
featureCounts
Rsubread
subread
CellBiology
updated 4.5 years ago by
Yang Liao
▴ 450 • written 4.5 years ago by
Jason
• 0
1
vote
5
replies
3.6k
views
Error: Input Files have different Amount of Reads
SubRead
Align
Subjunc
updated 5.2 years ago by
James W. MacDonald
68k • written 5.2 years ago by
abano
• 0
3
votes
4
replies
4.6k
views
Collecting unmapped RNA-seq reads
subread
read mapping
unmapped reads
written 6.0 years ago by
Patrick
• 0
0
votes
2
replies
1.4k
views
Problem in featureCounts file.
rna-seq
featureCounts
SubRead
updated 6.1 years ago by
Wei Shi
★ 3.6k • written 6.1 years ago by
hafiz.talhamalik
• 0
1
vote
2
replies
3.8k
views
Can't run with gff3 file
subread
featureCounts
gtf/gff/gff3
updated 6.2 years ago by
Gordon Smyth
53k • written 6.2 years ago by
algenubi81
• 0
4
votes
2
replies
1.7k
views
subread gives segfaults when supplied with falsely-identified paired end reads
subread
Rsubread
updated 6.4 years ago by
Gordon Smyth
53k • written 6.4 years ago by
Jonathan Griffiths
▴ 90
1
vote
1
reply
4.1k
views
featureCounts for ATAC-seq
subread
featurecounts
atac
updated 6.4 years ago by
Wei Shi
★ 3.6k • written 6.4 years ago by
aurel.nagy
▴ 10
0
votes
1
reply
1.3k
views
Index genome generation with Subread for colorspace data
subread
solid-seq data
colorspace
rna-seq
updated 6.4 years ago by
Yang Liao
▴ 450 • written 6.4 years ago by
gokberk
• 0
0
votes
5
replies
2.9k
views
featureCounts error : SAM_pairer_iterate_tags
subread
featureCounts
updated 6.6 years ago by
Gordon Smyth
53k • written 6.6 years ago by
leo_CD
• 0
4
votes
3
replies
1.6k
views
RSubread in cygwin
subread
6.7 years ago
Diana
▴ 10
0
votes
6
replies
2.1k
views
Options Request for FeatureCounts
featurecounts
subread
updated 7.0 years ago by
Wei Shi
★ 3.6k • written 7.1 years ago by
Leon
• 0
4
votes
5
replies
2.0k
views
Perfectly aligning sequences not mapping to worm genome with Subread-align?
featurecounts
rnaseq
subread
updated 7.1 years ago by
Gordon Smyth
53k • written 7.1 years ago by
rogangrant
• 0
3
votes
2
replies
3.6k
views
Subread FeatureCounts for Exon level quantification: Length estimate for Exons as low as 1 base-pair
subread
featurecounts
dexseq
rnaseq
updated 7.2 years ago by
Gordon Smyth
53k • written 7.2 years ago by
JennyS
• 0
3
votes
2
replies
2.9k
views
Reads mistakenly being assigned to Unassigned_NoFeatures category when using featureCounts
subread
featurecounts
updated 8.4 years ago by
Wei Shi
★ 3.6k • written 8.4 years ago by
jma1991
▴ 70
2
votes
2
replies
1.8k
views
reporting multi-mapping locations in Subread-align
subread
Rsubread
updated 9.7 years ago by
Wei Shi
★ 3.6k • written 9.7 years ago by
Vivek.b
▴ 100
33 results • Page
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Answer: readBam vs read_bam_insert_metrics
by
James W. MacDonald
68k
Depends on what you mean by input. If you mean to ask if you use `readBam` to generate something to use as an argument to `read_bam_insert_…
Answer: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
There is an example in the help page for `forgeBSgenomePkgFromTwobitFile`. Have you tried emulating that?
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
What you describe sounds a good approach to me. I would also use sample weights (`sample.weights=TRUE` when running `voomLmFit`) and robust…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Comment: Average biological replicates in RNA-seq
by
boczniak767
▴ 740
@atpoint Should I first standardise data and then average replicates?
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