Subcellular Spatial Transcriptomes: Emerging Frontier for Understanding Gene Regulation
- 1Center for Personal Dynamic Regulomes, Stanford University, Stanford, California 94305, USA
- 2Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305, USA
- Correspondence: howchang{at}stanford.edu
Abstract
RNAs are trafficked and localized with exquisite precision inside the cell. Studies of candidate messenger RNAs have shown the vital importance of RNA subcellular location in development and cellular function. New sequencing- and imaging-based methods are providing complementary insights into subcellular localization of RNAs transcriptome-wide. APEX-seq and ribosome profiling as well as proximity-labeling approaches have revealed thousands of transcript isoforms are localized to distinct cytotopic locations, including locations that defy biochemical fractionation and hence were missed by prior studies. Sequences in the 3′ and 5′ untranslated regions (UTRs) serve as “zip codes” to direct transcripts to particular locales, and it is clear that intronic and retrotransposable sequences within transcripts have been co-opted by cells to control localization. Molecular motors, nuclear-to-cytosol RNA export, liquid–liquid phase separation, RNA modifications, and RNA structure dynamically shape the subcellular transcriptome. Location-based RNA regulation continues to pose new mysteries for the field, yet promises to reveal insights into fundamental cell biology and disease mechanisms.
- © 2019 Fazal and Chang; Published by Cold Spring Harbor Laboratory Press
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