Ethel Mendocilla Sato

Ethel Mendocilla Sato

Zürich, Schweiz
557 Follower:innen 500+ Kontakte

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Creative thinker and proactive professional with over ten years’ experience in managing…

Aktivitäten

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Berufserfahrung

  • University of Zurich Grafik

    University of Zurich

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    Zurich, Switzerland

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    Zurich, Switzerland

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    Zürich Area, Switzerland

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    Zürich Area, Switzerland

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    Nottingham, UK

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    Nottingham, UK

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    Tours Area, France

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    Bordeaux Area, France

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    Lima Province, Peru

Ausbildung

  • Universiteit Antwerpen Grafik

    University of Antwerp

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    • Processed and organised large-scale image datasets to extract digital traits from images to interpret plant root architecture-related characteristics.
    • Developed a solid background in Bioinformatics and Statistics to analyse microarray data.
    • Presented research results in national and international seminars and conferences.
    • Applied and obtained funding from the European Plant Phenotyping Network (EPPN) to support access to phenotyping facilities at the University of Nottingham…

    • Processed and organised large-scale image datasets to extract digital traits from images to interpret plant root architecture-related characteristics.
    • Developed a solid background in Bioinformatics and Statistics to analyse microarray data.
    • Presented research results in national and international seminars and conferences.
    • Applied and obtained funding from the European Plant Phenotyping Network (EPPN) to support access to phenotyping facilities at the University of Nottingham (i.e. use of the robotised imaging setup coupled to RootTrace and the vertical confocal)

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Bescheinigungen und Zertifikate

Ehrenamt

  • PMI Switzerland Grafik

    Team event member

    PMI Switzerland

    –Heute 4 Jahre 3 Monate

  • Impact Hub Zürich Grafik

    Volunteer

    Impact Hub Zürich

    – 1 Monat

    • Participated in the virtual event - Climathon 2020.
    • Assisted participants as required.

  • Volunteer

    Impact Hub Basel

    – 1 Monat

    Umwelt

    • Participated in the Climathon 2019.
    • Assisted participants as required.

  • Life Sciences Switzerland (LS2) Grafik

    Volunteer Staff

    Life Sciences Switzerland (LS2)

    – 1 Monat

    Ausbildung

    • Participated in the Annual meeting organization.
    • Assisted chairpeople to ensure a smooth flow of the sessions.

  • Volunteer Staff

    Charity community services at the Belen's Hospital - Trujillo, Peru

    – 1 Jahr 4 Monate

    Sozialwesen

    • Visited patients in recovery rooms.
    • Assisted nurses with patient care.

  • Coach

    Horizon International solutions site - Trujillo, Peru

    – 1 Jahr 9 Monate

    Umwelt

    • Raised awareness about the impact of waste disposal in the environment.
    • Organized groups of people for picking up waste on the beach, forest.
    • Planned and organised fundraising events.

Veröffentlichungen

  • Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium

    eLife

    In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium…

    In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. We identified the spatio-temporal pattern of cell division that acts in a domain-specific manner as the primordium forms. Tissue growth models uncovered plausible morphogenetic principles involving a spatially confined growth signal, differential mechanical properties, and cell growth anisotropy. Our analysis revealed that SMC characteristics first arise in more than one cell but SMC fate becomes progressively restricted to a single cell during organ growth. Altered primordium geometry coincided with a delay in the fate restriction process in katanin mutants. Altogether, our study suggests that tissue geometry channels reproductive cell fate in the Arabidopsis ovule primordium.

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  • 3D imaging of whole-mount ovules at cellular resolution to study female germline development in rice

    Methods in Molecular Biology

    Recent advances in fluorescence-based staining of cellular compartments coupled with confocal microscopy imaging have allowed the visualization of three-dimensional (3D) structures with cellular resolution in various intact plant tissues and species. Such approaches are of particular interest for the analysis of the reproductive lineage in plants including the meiotic precursor cells deeply embedded within the ovary of the gynoecium enclosed in the flower. Yet, their relative inaccessibility…

    Recent advances in fluorescence-based staining of cellular compartments coupled with confocal microscopy imaging have allowed the visualization of three-dimensional (3D) structures with cellular resolution in various intact plant tissues and species. Such approaches are of particular interest for the analysis of the reproductive lineage in plants including the meiotic precursor cells deeply embedded within the ovary of the gynoecium enclosed in the flower. Yet, their relative inaccessibility and the lack of optical clarity of plant tissues prevent robust staining and imaging across several cell layers. Several whole-mount tissue staining and clearing techniques are available. One of them specifically allows staining of cellular boundaries in thick tissue samples while providing extreme optical clarity, using an acidic treatment followed by a modified Pseudo-Schiff propidium iodide (mPS-PI) method. While commonly used for Arabidopsis tissues, its application to other species like the model crop rice required protocol adaptations for obtaining robust staining that we present here. The procedure comprises six steps: (a) Material sampling; (b) Material fixation; (c) Tissue preparation; (d) Staining; (e) Sample mounting; and (d) Microscopy imaging. Particularly, we use ethanol and acetic anhydride as fixative reagents. A modified enzymatic treatment proved essential for starch degradation influencing optical clarity hence allowing acquisition of images at high resolution. This improved protocol is efficient for analyzing the megaspore mother cells in rice (Oryza sativa) ovary but is broadly applicable to other crop tissues of complex composition, without the need for tissue sectioning.

    Andere Autor:innen
    • She W
    • Baroux C
    Veröffentlichung anzeigen

  • Analysis of 3D cellular organization of fixed plant tissues using a user-guided platform for image segmentation

    Bio-protocol

    Veröffentlichung anzeigen

  • New insights into the dynamics of root gravitropic signaling

    Journal of Experimental Botany

    An important feature of plants is the ability to adapt their growth towards or away from external stimuli such as light, water, temperature, and gravity. These responsive plant growth movements are called tropisms and they contribute to the plant's survival and reproduction. Roots modulate their growth towards gravity to exploit the soil for water and nutrient uptake, and to provide anchorage. The physiological process of root gravitropism comprises gravity perception, signal transmission…

    An important feature of plants is the ability to adapt their growth towards or away from external stimuli such as light, water, temperature, and gravity. These responsive plant growth movements are called tropisms and they contribute to the plant's survival and reproduction. Roots modulate their growth towards gravity to exploit the soil for water and nutrient uptake, and to provide anchorage. The physiological process of root gravitropism comprises gravity perception, signal transmission, growth response, and the re-establishment of normal growth. Gravity perception is best explained by the starch-statolith hypothesis that states that dense starch-filled amyloplasts or statoliths within columella cells sediment in the direction of gravity, resulting in the generation of a signal that causes asymmetric growth. Though little is known about the gravity receptor(s), the role of auxin linking gravity sensing to the response is well established. Auxin influx and efflux carriers facilitate creation of a differential auxin gradient between the upper and lower side of gravistimulated roots. This asymmetric auxin gradient causes differential growth responses in the graviresponding tissue of the elongation zone, leading to root curvature. Cell biological and mathematical modelling approaches suggest that the root gravitropic response begins within minutes of a gravity stimulus, triggering genomic and non-genomic responses. This review discusses recent advances in our understanding of root gravitropism in Arabidopsis thaliana and identifies current challenges and future perspectives.

    Andere Autor:innen
    Veröffentlichung anzeigen

  • Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping point mechanism

    PNAS

    Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses to modify their growth. Cholodny and Went hypothesized over 80 years ago that plants bend in response to a gravity stimulus by generating a lateral gradient of a growth regulator at an organ's apex, later found to be auxin. Auxin regulates root growth by targeting Aux/IAA repressor proteins for degradation. We used an Aux/IAA-based reporter, domain II (DII)-VENUS…

    Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses to modify their growth. Cholodny and Went hypothesized over 80 years ago that plants bend in response to a gravity stimulus by generating a lateral gradient of a growth regulator at an organ's apex, later found to be auxin. Auxin regulates root growth by targeting Aux/IAA repressor proteins for degradation. We used an Aux/IAA-based reporter, domain II (DII)-VENUS, in conjunction with a mathematical model to quantify auxin redistribution following a gravity stimulus. Our multidisciplinary approach revealed that auxin is rapidly redistributed to the lower side of the root within minutes of a 90° gravity stimulus. Unexpectedly, auxin asymmetry was rapidly lost as bending root tips reached an angle of 40° to the horizontal. We hypothesize roots use a "tipping point" mechanism that operates to reverse the asymmetric auxin flow at the midpoint of root bending. These mechanistic insights illustrate the scientific value of developing quantitative reporters such as DII-VENUS in conjunction with parameterized mathematical models to provide high-resolution kinetics of hormone redistribution.

    Andere Autor:innen
    Veröffentlichung anzeigen

Kurse

  • Business concept

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  • Careers in Science or Policy, or both

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  • How to publish in peer-reviewed journals

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  • Intellectual Property

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  • Project Management

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  • Successful fund acquisition for researchers

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  • Successful proposal writing for researchers

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  • Voice training and presentation skills for postdocs

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  • Volume scanning electron microscopy - practical course in Advanced Microscopy

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Projekte

  • Role of cellular morphodynamics in cell fate acquisition during germline formation in Arabidopsis and rice

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  • Molecular characterisation of the auxin response during root gravitropism

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  • Characterisation of genes and proteins involved in the synthesis of galactomannans during coffee berry development.

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  • Analysis of metabolic profiles of tomato leaves by 1H-Nuclear Magnetic Resonance (NMR) Spectroscopy

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Auszeichnungen/Preise

  • European Plant Phenotyping Network (EPPN) grant

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  • Research Foundation Flanders (FWO) doctoral fellowship

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Sprachen

  • English

    Verhandlungssicher

  • French

    Verhandlungssicher

  • Spanish

    Muttersprache oder zweisprachig

  • German

    Gute Kenntnisse

Organisationen

  • Project Management Institute

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  • Project Management Institute Switzerland Chapter

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