Maurizio Bettiga

Maurizio Bettiga

Milano, Lombardia, Italia
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Articoli di Maurizio

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Attività

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Esperienza

  • Italbiotec Srl

    Novara, Piedmont, Italy

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    Milan, Lombardy, Italy

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    Milan Area, Italy

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    Göteborg

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    Gothenburg, Sweden

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    Bari Area, Italy

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    Bari, Apulia, Italy

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    Bari Area, Italy

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    Gothenburg, Sweden

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    Lund, Sweden

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    Milan Area, Italy

Formazione

  • Grafico Chalmers University of Technology

    Chalmers University of Technology

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    Swedish highest academic title.
    Qualification as Docent requires, in addition to a PhD, substantially greater documented independent ability to lead in formulating and solving scientific research problems, as well as pedagogical competences at the advanced graduate study level.

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    Title of the thesis: “Involvement of the chromatin deubiquitinating enzyme Ubp10 in apoptosis and aging in yeast”. (January 2006, with honors).

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    MSc in Industrial Biotechnology (Biotecnologie Industriali)

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Pubblicazioni

  • Membrane engineering of S. cerevisiae targeting sphingolipid metabolism

    Scientific Reports

    The sustainable production of fuels and chemicals using microbial cell factories is now well established. However, many microbial production processes are still limited in scale due to inhibition from compounds that are present in the feedstock or are produced during fermentation. Some of these inhibitors interfere with cellular membranes and change the physicochemical properties of the membranes. Another group of molecules is dependent on their permeation rate through the membrane for their…

    The sustainable production of fuels and chemicals using microbial cell factories is now well established. However, many microbial production processes are still limited in scale due to inhibition from compounds that are present in the feedstock or are produced during fermentation. Some of these inhibitors interfere with cellular membranes and change the physicochemical properties of the membranes. Another group of molecules is dependent on their permeation rate through the membrane for their inhibition. We have investigated the use of membrane engineering to counteract the negative effects of inhibitors on the microorganism with focus on modulating the abundance of complex sphingolipids in the cell membrane of Saccharomyces cerevisiae. Overexpression of ELO3, involved in fatty acid elongation, and AUR1, which catalyses the formation of complex sphingolipids, had no effect on the membrane lipid profile or on cellular physiology. Deletion of the genes ORM1 and ORM2, encoding negative regulators of sphingolipid biosynthesis, decreased cell viability and considerably reduced phosphatidylinositol and complex sphingolipids. Additionally, combining ELO3 and AUR1 overexpression with orm1/2Δ improved cell viability and increased fatty acyl chain length compared with only orm1/2Δ. These findings can be used to further study the sphingolipid metabolism, as well as giving guidance in membrane engineering.

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  • A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae

    Bioresource Technology

    The conversion of coniferyl aldehyde to cinnamic acids by Saccharomyces cerevisiae under aerobic growth conditions was previously observed. Bacteria such as Pseudomonas have been shown to harbor specialized enzymes for converting coniferyl aldehyde but no comparable enzymes have been identified in S. cerevisiae. CALDH from Pseudomonas was expressed in S. cerevisiae. An acetaldehyde dehydrogenase (Ald5) was also hypothesized to be actively involved in the conversion of coniferyl aldehyde under…

    The conversion of coniferyl aldehyde to cinnamic acids by Saccharomyces cerevisiae under aerobic growth conditions was previously observed. Bacteria such as Pseudomonas have been shown to harbor specialized enzymes for converting coniferyl aldehyde but no comparable enzymes have been identified in S. cerevisiae. CALDH from Pseudomonas was expressed in S. cerevisiae. An acetaldehyde dehydrogenase (Ald5) was also hypothesized to be actively involved in the conversion of coniferyl aldehyde under aerobic growth conditions in S. cerevisiae. In a second S. cerevisiae strain, the acetaldehyde dehydrogenase (ALD5) was deleted. A prototrophic control strain was also engineered. The engineered S. cerevisiae strains were cultivated in the presence of 1.1 mM coniferyl aldehyde under aerobic condition in bioreactors. The results confirmed that expression of CALDH increased endogenous conversion of coniferyl aldehyde in S. cerevisiae and ALD5 is actively involved with the conversion of coniferyl aldehyde in S. cerevisiae.

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  • Sphingolipids contribute to acetic acid resistance in Zygosaccharomyces bailii

    Biotechnol. Bioeng.

    Lignocellulosic raw material plays a crucial role in the development of sustainable processes for the production of fuels and chemicals. Weak acids such as acetic acid and formic acid are troublesome inhibitors restricting efficient microbial conversion of the biomass to desired products. To improve our understanding of weak acid inhibition, and to identify engineering strategies to reduce acetic acid toxicity, the highly acetic-acid-tolerant yeast Zygosaccharomyces bailii was studied. The…

    Lignocellulosic raw material plays a crucial role in the development of sustainable processes for the production of fuels and chemicals. Weak acids such as acetic acid and formic acid are troublesome inhibitors restricting efficient microbial conversion of the biomass to desired products. To improve our understanding of weak acid inhibition, and to identify engineering strategies to reduce acetic acid toxicity, the highly acetic-acid-tolerant yeast Zygosaccharomyces bailii was studied. The impact of acetic acid membrane permeability on acetic acid tolerance in Z. bailii was investigated with particular focus on how the previously demonstrated high sphingolipid content in the plasma membrane influences acetic acid tolerance and membrane permeability. Through molecular dynamics simulations we concluded that membranes with a high content of sphingolipids are thicker and more dense, increasing the free energy barrier for the permeation of acetic acid through the membrane. Z. bailii cultured with the drug myriocin, known to decrease cellular sphingolipid levels, exhibited significant growth inhibition in the presence of acetic acid, while growth in medium without acetic acid was unaffected by the myriocin addition. Furthermore, following an acetic acid pulse, the intracellular pH decreased more in myriocin-treated cells than in control cells. This indicates a higher inflow rate of acetic acid, and confirms that the reduction in growth of cells cultured with myriocin in the medium with acetic acid, was due to an increase in membrane permeability, thereby demonstrating the importance of a high fraction of sphingolipids in the membrane of Z. bailii to facilitate acetic acid resistance; a property potentially transferable to desired production organisms suffering from weak acid stress.

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  • Catabolism of coniferyl aldehyde, ferulic acid and p-coumaric acid by Saccharomyces cerevisiae yields less toxic products

    Microbial Cell Factories 14:149

    Altri autori
    • Peter Temitope Adeboye
    • Fredrik Aldaeus
    • Per Tomas Larsson
    • Lisbeth Olsson
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  • The chemical nature of phenolic compounds determines their toxicity and induces distinct physiological responses in Saccharomyces cerevisiae in lignocellulose hydrolysates

    AMB Express

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  • Engineering glutathione biosynthesis of Saccharomyces cerevisiae increases robustness to inhibitors in pretreated lignocellulosic materials

    Microbial Cell Factories

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  • Lipidomic Profiling of Saccharomyces cerevisiae and Zygosaccharomyces bailii Reveals Critical Changes in Lipid Composition in Response to Acetic Acid Stress

    PLOS one

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  • The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae

    Biotechnology For Biofuels

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  • Isolation and characterization of a resident tolerant S. cerevisiae strain from a spent sulfite liquor fermentation plant.

    AMB Express 2(1):68.

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  • A microbial perspective on ethanolic lignocellulose fermentation.

    Comprehensive Biotechnology, 2nd Edition, Elsevier

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Corsi

  • 2-Dimensional Protein Electrophoresis

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  • Applied Project Management (by Wenell AB)

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  • Bioanalytical HPLC

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  • Biogas Technology for Second Generation Biofuels Production

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  • Bioreaction Engineering

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  • Gene Expression Analysis-Affymetrix GeneChip technology

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  • Handledare forum (Supervisors forum)

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  • Leadership (Chalmers Leadership Program)

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  • Proteome analysis

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  • Supervision in Research

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  • The Teaching Portfolio

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