Live demonstration: Real-time calcium trace extraction from large-field-of-view miniscope

Z Chen, GJ Blair, C Guo, D Aharoni… - … Circuits and Systems …, 2021 - ieeexplore.ieee.org
2021 IEEE Biomedical Circuits and Systems Conference (BioCAS), 2021ieeexplore.ieee.org
We demonstrate a prototype system ACTEV for acceleration of calcium trace extraction from
video captured by a large-field-of-view (LFOV) Miniscope at 22.8 fps. The ACTEV consists of
an FPGA accelerator for the calcium image processing, an interface PCB for the data
transmission and a GUI software for the user interaction. The FPGA accelerator takes a
stream of 512× 512 calcium images as input, executes motion correction, image
enhancement and trace extraction from 1024 cells per frame with< 1ms latency. The PCB …
We demonstrate a prototype system ACTEV for acceleration of calcium trace extraction from video captured by a large-field-of-view (LFOV) Miniscope at 22.8 fps. The ACTEV consists of an FPGA accelerator for the calcium image processing, an interface PCB for the data transmission and a GUI software for the user interaction. The FPGA accelerator takes a stream of 512 × 512 calcium images as input, executes motion correction, image enhancement and trace extraction from 1024 cells per frame with <1ms latency. The PCB transmits the raw calcium video and extracted traces to the host computer over an Ethernet interface. The GUI displays the received images and traces simultaneously for the user. The user can observe the motion corrected video and extracted traces from individual cells as the rat freely runs on a linear track.
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