The ability to make behavioural inferences from skeletal remains is critical to understanding the lifestyles and activities of past human populations and extinct animals. Muscle attachment site (enthesis) morphology has long been assumed...
moreThe ability to make behavioural inferences from skeletal remains is critical to understanding the lifestyles and activities of past human populations and extinct animals. Muscle attachment site (enthesis) morphology has long been assumed to reflect muscle strength and activity during life, but little evidence exists to directly link activity patterns with muscle development and the morphology of their attachments to the skeleton. This research used a primate sample (Pongo pygmaeus abelii, Macaca fuscata, and Macaca sylvanus) and an experimental mouse model (Mus musculus, CD-1 wild-type female outbred) to test how locomotor and postural behaviours influence the development of the forelimb muscles, bone growth rate, bone remodelling, crosssectional geometry, and the gross morphology of their humeral attachment sites. A total of twenty-two muscles attaching to the humerus from the three species of primates were analysed. No consistent patterns between muscle architecture and entheseal morphology were found, although considering the underlying cortical bone of an attachment site seemed more informative of the associated muscle. Results demonstrate how variable muscle and bone can be even when considering factors such as age, sex, species, body mass, and locomotion. iii Over an eleven-week period, data was collected on the activity levels in one control group and two experimental activity groups (running, climbing) of female mice. The three muscles attaching to the deltoid crest (spinodeltoideus, acromiodeltoideus and superficial pectoralis) were examined. Results show that both activity type and level increased bone growth rates, remodelling and muscle architecture, including differences in potential muscular excursion (fibre length) and potential force production (physiological cross-sectional area). However, despite significant influences on muscle and bone development, activity had no observable effect on enthesis size or morphology. The results of this study suggest that the gross morphology of entheses is less reliable than the internal bone structure for making inferences about an individual's past behaviour. Further knowledge of the functional significance and development of entheseal morphology is needed if one is to accurately reconstruct behaviour based solely on skeletal and fossil remains. I am indebted to Drs. David Green, Shannon McFarlin, and Brian Richmond for kindly providing the mouse sample, along with their guidance and expertise for the hard tissue analyses. For their mentorship, friendship, and collaboration I am tremendously grateful. I am also very thankful to Drs. Bernard Wood and Chet Sherwood for their support and guidance during my stay in Washington DC. I was very lucky to have met a wonderful group of graduate students, postdocs, and researchers at GW who have given me their support, time, and extremely fond memories. I thank all of the "CASHP" gang I have met, especially Amy Bauernfeind, Cheryl Stimpson, Janine Chalk, Erin Marie Williams, Kevin Hatala, and Andrew Du. You are amazing and made my stay in the U.S. so much fun. The data presented in Chapters 5 and 6 would not be possible without the help, time, and collections given to me by many individuals and institutions. Thanks are due to Dr. Timothy Bromage for taking time to explain many of the microscopic concepts that I used to analyse the mouse sample. Also, I would like to thank Drs. Andrea Taylor and Mark Teaford who have opened their home and lab for me while my many visits to Duke University for sarcomere analysis. Andrea has been endlessly helping me with my thesis throughout the years, and I look forward to more collaboration with her. Thanks are due to Stephanie Holowka who volunteered her time at the Hospital for Sick Children to CT scan the primate sample. Despite her busy schedule, Stephanie always found extra time to have me come in with all of the bones and always had a big smile and great stories to share. A special thank you goes to the museums and their collection managers who granted me access to their skeletal remains and kindly coordinated my visits: Eileen Westwig (American Museum of Natural History), Linda Gordon (National Museum of Natural History), Judy Chupasko (Museum Comparative Zoology), and Susan Woodward (Royal Ontario Museum). I would also like to express my gratitude to Rob Javonillo vii and Judy Chupasko for opening their homes during my data collection in Washington and in Boston respectively. I am greatly in debt to Graham Crawshaw and Dyann Powley from the Toronto Zoo for donating to the lab their primates. They were always open to have me come work in their facilities, and have helped me immensely with all of the details that come with the research of primate tissue. Finally, I would like to thank all the members of the Archaeology Centre at the University of Toronto, the Hard Tissue Research Laboratory at the George Washington University, and the Soft Tissue Laboratory at Duke University for graciously providing access to their labs and equipments. The analyses of this dissertation would not have been possible without your help, time, and dedication. A huge thank you also goes to all my family