Piotr Kowalski’s Post

Excited to see the results of our collaboration with the Marama Labs lead by my PhD student Aida López Espinar published in Analytical Chemistry Journal from American Chemical Society. 👏 Have you ever struggled with accuracy, precision, and reproducibility of the RNA quantification in NPs using fluorescence-based methods❓ 👇 ◾ In this study, we have investigated a novel scatter-free absorption spectroscopy (SFAS) method for total RNA quantification in complex nanoparticle formulations, including lipid, polymer, dendrimer, and cyclodextrin nanoparticles ◾ We showcased the superiority of SFAS for total RNA quantification compared to fluorescence-based assays like RiboGreen and SYTO 9. ◾ We found a strongly improved accuracy of SFAS for the formulations that resist disruption by detergents Open Access article available: https://lnkd.in/e7ukv7jq Many thanks to all the co-authors, collaborators, and funders who supported our work, especially Brendan Darby, Eric Le Ru, and Caitriona O'Driscoll School of Pharmacy, UCC, APC Microbiome Ireland, Health Research Board (HRB), GENEGUT, European Research Council (ERC), ACS Publications, ATMP Ireland, Parveen Kumar, Ana Francisca Soares #SFAS, #RNA, #LNP, #RiboGreen, #cyclodextrin #Polymer