DNBseqTM SERVICE OVERVIEW
10-Day Rapid Delivery Whole Exome Sequencing
DNBseq™ rWES service
Library cosntruction DNBseq™ PE100
DNA sample QC Data processing
and exon capture sequencing
Service Description
For many applications, Whole Exome Sequencing (WES) offers advantages as a viable and cost-effective alternative for Whole
Genome Sequencing. With the development of precision medicine, WES is utilized more and more frequently in patient cases
where the diagnoses or treatment options are unclear. Timely diagnosis can aid in treatment efficacy and better patient health
outcomes.
BGI is now offering a 10-day rapid delivery whole exome sequencing service from our CAP/CLIA certified laboratory to meet
the needs of both academic and clinical research users where rapid delivery of results is paramount. Besides raw sequencing
data output, BGI also offers standard and custom bioinformatics services to suit specific research needs.
DNBseq™ Technology
Whole Exome Sequencing services are performed with BGI’s proprietary DNBseq™ sequencing technology platforms. DNBseq™
is a high-throughput sequencing platform developed by a subsidiary of BGI, Complete Genomics, in Silicon Valley. This system
is powered by combinatorial Probe-Anchor Synthesis (cPAS), linear isothermal Rolling-Circle Replication and DNA Nanoballs
(DNB™) technology, followed by high-resolution digital imaging.
BGI Advantages
◆ Project experience of tens of thousands of exome samples
◆ Lower sequencing cost and lower index hopping rate by using DNBseq™ technology
Sequencing Service Specification
◆ Agilent Sureselect or BGI exome kit for library construction and enrichment
◆ 100bp paired-end sequencing options available
10 Day Rapid Delivery Whole Exome Sequencing
Data Performance
The following is an example of rapid DNBseq™ output for a 100X WES project with standard sample NA12878. The capture kit
used was Agilent Human All Exon V7, and each sample generated roughly 10G of data.
Coverage performance
100
90
80
70
60
Mapping rate(%) Fraction of target Fraction of target Fraction of target
covered >= 4x (%) covered >= 10x (%) covered >= 20x (%)
NA12878-1 NA12878-2
High repeatability, precision and sensitivity of SNP calling. High repeatability, precision and sensitivity of InDel calling.
NA12878-1 NA12878-2 NA12878-1 NA12878-2
36760 3493
725 96.1%
771 401 81.03%
417
1.9% 2.0% 9.3% 9.7%
Sample Precision Sensitivity Sample Precision Sensitivity
NA12878-1 99.2% 97.5% NA12878-1 88.1% 90.0%
NA12878-2 99.2% 97.7% NA12878-2 86.7% 89.4%
Request for Information or Quotation
Contact your BGI account representative for the most affordable rates in the industry and to discuss how we can
meet your specific project requirements or for expert advice on experiment design, from sample to bioinformatics.
[email protected] www.bgi.com
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Copyright ©2019 BGI. The BGI logo is a trademark of BGI. All rights reserved.
All brand and product names are trademarks or registered trademarks of their respective holders.
DNBseq™ is a trademark of MGI Co. Ltd.
Information, descriptions and specifications in this publication are subject to change without notice.
Published June 2019.
All services and solutions are for research use only. We Sequence, You Discover
