Benson: Microbiological Back Matter Laboratory Reports © The McGraw−Hill
Applications Lab Manual, Companies, 2001
Eighth Edition
Student: ___________________________________
LABORATORY REPORT
Desk No.: __________ Section: ______________
78
The Staphylococci:
Isolation and Identification
A. Tabulation
At the beginning of the third laboratory period, the instructor will construct a chart similar to this one on the
chalkboard. After examining your mannitol salt agar and staphylococcus medium 110 plates, record the pres-
ence (⫹) or absence (⫺) of staphylococcus growth in the appropriate columns. After performing coagulase
tests on the various isolates record the results also as (⫹) or (⫺) in the appropriate columns.
397
Benson: Microbiological Back Matter Laboratory Reports © The McGraw−Hill
Applications Lab Manual, Companies, 2001
Eighth Edition
The Staphylococci: Isolation and Identification
B. Microscopy
Provide drawings here of the various isolates as seen under oil immersion (gram staining).
C. Percentages
From the data in the table on the previous page, determine the incidence (percentage) of individuals and
fomites that harbor coagulase-positive and coagulase-negative staphylococci in this experiment.
D. Record of Test Results
Record here the results of each test performed in this experiment. Under GRAM STAIN indicate cellular
arrangement as well as Gram reaction.
E. Final Determination
Record here the name of your unknown-control. If API Staph-Ident miniaturized multitest strips are avail-
able, confirm your conclusions by testing each isolate. See Exercise 55.
Name of unknown-control: ___________________________________________________________
Staph-Ident results: _________________________________________________________________
F. Questions
1. What are nosocomial infections? ________________________________________________________
2. What bacterial organism causes most nosocomial infections?__________________________________
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