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Quantity of Casein

This document summarizes a chemistry project that analyzed the quantity of casein in different milk samples. The project involved adding acetic acid to milk samples to precipitate the casein protein, then collecting, drying, and weighing the casein to calculate the percentage in each sample. The results showed that casein levels varied between brands, with Amul brand containing the highest level at 4.20%. In conclusion, the project demonstrated that different milk samples contain different percentages of the important casein protein.

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0% found this document useful (0 votes)
141 views3 pages

Quantity of Casein

This document summarizes a chemistry project that analyzed the quantity of casein in different milk samples. The project involved adding acetic acid to milk samples to precipitate the casein protein, then collecting, drying, and weighing the casein to calculate the percentage in each sample. The results showed that casein levels varied between brands, with Amul brand containing the highest level at 4.20%. In conclusion, the project demonstrated that different milk samples contain different percentages of the important casein protein.

Uploaded by

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3/25/2016 Chemistry 

Project (CBSE) 12th class Quantity of Casein in Different Samples of Milk Contains Vitamins, Proteins, Carbohydrates, Fats and the Maj…

Chemistry Project Report on Study of Quantity of Casein Present in Various
Samples of Milk

 
 
Submitted by:
Student of XII­Science (Chemistry)

 
 
Submitted to :
Lecturer in Chemistry 
Sr. Sec. School

Index

(a) Introduction
(b) Requirements
(c) Procedure
(d) Observation
(e) Result and discussion
(f) Bibliography

Certificate

This  is  certified  that  the  chemistry  project  report  entitled  "A  project  on  the  study  of
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3/25/2016 Chemistry Project (CBSE) 12th class Quantity of Casein in Different Samples of Milk Contains Vitamins, Proteins, Carbohydrates, Fats and the Maj…

quantity of casein in different samples of milk" submitted for partial fulfillment of XII Sc.
Practical examination in the subject of Chemistry embodies a record of original study carried
out by student under my Supervision.

This project in my opinion is completed and is suitable for presentation.

Lecturer in Chemistry.

Introduction

Milk is a white liquid secreted by the mammary glands of female cows, buffaloes etc. It is a
food of exceptional inters probability. Milk is the most nutritious food found in nature. That is
why it is widely consumed both in its natural forms and as diary product like butter, cheese
etc.

Milk contains Vitamins, Proteins, Carbohydrates and the major milk protein casein are found
only in milk and nowhere else in nature. The Composition of casein in milk is not constant. It
depends upon the Sources of Milk.

Theory

The Protein,  Casein,  Existent,  Milk as  the  calcium  salt,  calcium  casein  etc.  The  salt  has  a
complex  structure  and  form  micelles.  These  micelles  have  negative  charges  on  the  outer
surface.

Requirements :

250­ml. beaker, filtrations flask, measuring cylinder, glass rod, spatula, china dish, dropper,
and weight bore, different samples of milk, 10% acetic acid.

Procedure :

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3/25/2016 Chemistry Project (CBSE) 12th class Quantity of Casein in Different Samples of Milk Contains Vitamins, Proteins, Carbohydrates, Fats and the Maj…

1. Measure 20­ml, of a milk sample with the help of measuring cylinder and transfer into a
clean 250ml. beaker.
2. Make the volume of milk to about 35ml, by adding water and warm the mixture to about
40 c.
3. Add  to  the  warm  mixture  drowse  and  with  constant  stirring  10%  acetic  acid  unit  the
precipitation is completed.
4. Stir  the  mixture  for  another  10  minutes  or  till  the  precipitated  casein  forms  a  large
amorphous mass.
5. Filter the casein precipitate.
6. Wash thoroughly with water, dry foils between folds of filter paper and then in air for 1­2
days weight the dry solid so obtained.
7. Similarly, determine the amount of casein in other samples of milk.

Observation :

Volume of Milk Taken in each case = 20ml.

Sample No. Quality of Milk Weight of casein percentage of casein


1. SARAS 0.60 3.00%
2 PANCHAMRAT 0.65 3.25%
3. AMUL 0.85 4.20%
4. RATH 0.75 3.70

Therefore  if  an  acid  is  added  to  milk  the  negative  charge  are  neutralized  and  the  neutral
protein precipitated out.

Ca+2 (casemated) +2 CH3 COOH (aq) 
                                            Casein + (CH3 COO)2 Ca

Results and Discussion :

Different samples of milk contain different percent of casein and the casein contents in maul
brand are appreciable. At present amul is the best brand of milk available in the market.

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