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LESSON 2
INFECTION CONTROL, SAFETY,
FIRST AID, AND PERSONAL
B59
LEARNING OUTCOMES.
At the end of this lesson, the students must be able to:
1
5. identify symptoms of shock, firs
14 Read Information Sheet 2.0.
Infection Control, Safety, First Ald,
‘and Personal Wellness
21. Components of the Chain of infection, Infection-
2.2. Proper Procedures of Hand Hygiene, Putting on
23. Standard and Transmission Based
identify the components of the chain of infection and give
examples of each, describe infection-control procedures used to
break the chain of infection, and explain the four functions of
infection-control program;
perform proper procedures for hand hygiene, putting on and
removing protective clothing, and entering the ORs and [CUS
. discuss the standard and transmission-based precautions for
blood-borne pathogens;
list examples of blood-borne pathogens and describe their means
of transmission in a healthcare settings
describe hazards, identify warning symbols, list actions to take if
incidents occur, and specify rules to follow for proper biological,
electrical, fire, radiation, and chemical safety;
procedures, and the main
points of the international cardiopulmonary resuscitation (CPR);
and
. explain the role of personal hygiene, proper nutrition, rest,
exercise, back protection, and stress management in personal
wellness
I LEARNING GUIDE
Learning Steps Time Allotment
Control Program, and Intection-Control Procedures.
end Removal of Protective Coothing, and Entering
the Operation Rooms (ORS) and intensive Care
Units cus)
Precaution for Blood-Bome Pathogens
UERMMMCI LIBRARY 99 14592*
co Rate tela. OC
Information Sheet 2.016
Learning Guide for Principles of Medical Laboratory Science 2
4 Han
ifineldonts Occur
15, Warning Symbo's, Actions to Tako
2.5. Symptoms of Shock, Fist
‘and Cardiopulmonary Resusctation (CPR)
26. Personal Wellness
Resource
1nd Laboratory Safety Risks
Procecure,
2, Suogested SeifDirected Loatning Activities: 30nours | instructor/rainer/
‘Attend lecture session, reod related articles, and Speakor/Stucent Porta,
‘Watch porta-based presentation on "Understanding LUbrary/Simulated
Phlebotomy” or observe the set-up and operations of a Clinical Laboratory
‘neorby clinica laboratory (optiona Laboratory Actwity
Actnity #1 (Page 169)
3. Perform portal based Self: Chock Activity 2.0. Student Portel
‘4. Perform portal-based summative examination ‘Student Portal
for Lesson 2. paces
5. Roport to and consult with your instructor regarding ‘Summaiive examination
your examination results, rosuits
6. Proceed to Lesson
Lesson 3
[BPS] INFORMATION SHEET 2.0
Although phlebotomy is considered one of the most commonly
performed procedures in the field of medicine, there are still risks
involved that can be hazardous to both patient and healthcare personnel.
When not done properly, this procedure could result in injury of the
patient, errors in laboratory findings and exposure to infection, and other
safety hazards. Risk could be reduced if best practices in prevention and
control are followed.
An infection happens when a microorganism invades the body,
multiplies, and causes injury or disease. A pathogen is a disease-causing
microbe which could be classified as bacteria, fungi, protozoa, or
Virus. There are two types of infections: communicable infections and
‘nosocomial and healthcare-associated infections (HAs). Communicable
infections can spread from person to person while nosocomial and
healthcare-associated infections (HAls) are usually caused by infected
personnel, patients, visitors, food, drug, or equipment while a patient is in
the hospital or other healthcare facilities.
Components of the Chain of Infection
For the infection to escalate to an epidemic, all components or links
should be favorable to the pathogenic microbe. The following table shows
the six links of the chain of infection and their description.Infection Control, Safety, First Ald, and Personal Wellness,
Infectious Pathogenic microbe such os virus, Bacteria, fungus, protozoa, and ricketsia
(causative agent | oer
Reservoir Source of tre agent of infection or place where the microbe could grow, sunive.
‘and mutiply, which could be in humans, imal, food, water, sol or equipment
Extpathway | Away or manner wherein an infectious agent can leave the reservoir host, which
could be through secretions and enidatos, tesve specimens, Diood, feces, oF uring
Means of ‘Aroome, direct touching oF kissing) or indirect contact (contaminated objects).
transmission | droplets (coughing or sneezing), vector insect, anthropod, or animal, and vehicle
(food, water, or drugs) were Ca
Entry pathway | The way an infectious agent enters a host, which includes body orfices, mucous
membrones, end breaks inthe skin
Susceptible host | Someone who is prone to infecton, expec the elderly, newborn babies,
eteents who ave immune-suppressed or unvaccinated, and those suffering from
acute 0 cone iiness| -
Infection Control Program
A healthcare institution should have a set of procedures to break
the chain of infection. These measures include effective hand hygiene
procedure, good nutrition, immunization against common pathogens,
insect and pest control, isolation and decontamination procedures, use
of proper safety-devices, wearing of personal protective equipment (PPE)
when needed, and proper disposal of sharp objects and other waste
materials.
An infection-control program has four main functions:
1. to protect patients, employees, and visitors from infection
2. to screen employees for infectious diseases and to require
immunization when needed
3. to provide cvaluation and treatment to health workers who have
been exposed to infections while performing their duty
4. to monitor employees and patients who are at risk of infection
and to collect data from patients and health workers who have
bbeen exposed to such danger
Infection-control Procedures
Infection-control methods or procedures must be followed at all
times. These include ensuring that proper hand hygiene is practiced
consistently using alcohol-based antiseptic hand cleaners, and that the
Personal protective equipment (PPE) is clean and properly donned
and removed.
748 Leaming Guide for Principles of Medical Laboratory Science 2
Proper Procedures of Hand Hygiene,
Putting on and Removal of Protective Clothing,
and Entering the Operating Rooms (ORs)
and Intensive Care Units (ICUs)
Hand Hygiene
Hand hygiene is an essential part of standard precautions in the
healthcare setting because it is an effective way to prevent infections that
can be transmitted from the patient and healthcare personnel during the
procedure, There are tivo methods for hand hygiene:
1, routine hand washing
+ uses plain soap and water
+ when hands are visibly dirty
+ after known exposure to Clostridium difficile, Bacillus
anthracis, and infectious diarrhea during norovirus outbreaks
«+ before eating
+ after using the restroom
2, hand antisepsis
‘+ an antimocrobial soap or alcohol-based hand sanitizer to
remove transient microorganisms x
+ alcohol-based hand sanitizer is preferred when hands are not
visibly dirty
* put the sanitizer on hands, rub the hands together for about
20 seconds or until it feels dryInfection Control, Safety, First Aid, and Personal Wellness 19
Steps in Performing the Routine
Hand-washing Technique
1, Stand a few inches from the sink to avoid contamination.
2. Turn on the faucet and place hands under the running water.
3. Use soap and work up lather to ensure that hand surfaces are reached,
4, Scrub for at least 15 seconds. Make sure to scrub all surfaces
especially between the fingers and the knuckles.
5. Apply alittle friction and rub hands together for atleast 15 seconds.
6. Rinse the hands from the wrist to fingertips using a downward
motion,
7. Dry hands using a clean paper towel.
8, Use the paper towel to cose the faucet, except when the latter is foot-
or motion-activated.
Personal Protective Equipment (PPE)
‘The personal protective equipment (PPE) includes the gloves, gowns,
lab coats, masks, face shields, goggles, and respirators.
Gloves
Phlebotomists should always wear gloves during blood collection and
‘when handling specimen. Gloves are worn to prevent contamination of
the hands and reduce chances of transmission of microorganisms from
personnel to patients. They should be worn over the cuffs of the ab gown
to ensure protection.
Proper Removal of Gloves
1. The wrist part of one glove is grasped by the opposite hand.
2, The glove is pulled inside out and off the hand.
3. The recently removed glove must be placed in the gloved hand. The
fingers of the non-gloved hand are slipped under the wrist of the
remaining glove but make sure not to touch the exterior surface.
4. The second glove is pulled inside out.
5. The gloves must be dropped in the proper receptacle.
Figure 2.1 Proper Personal
Protective Equipment (PPE)
@Head cap, @:
gogglesiglasses, @Face
coat, @Gioves, @Long pants,
@ciosed-toe shoes
[Counesy of he Scxoot
of Medical Technology,
Contr Eecolar Universty)20
Leaming Guide for Principles of Medical Laboratory Science 2
Figure 2.2 Disposable
Mask
Figure 2.3 Disposable
Gloves
Figure 2.4 Disposable
Laboratory Gown
Figure 2.5 PPE Worn
Inthe Operating Room
Donning and Doffing of PPE
The PPE is kept clean and is worn to protect the healthcare
worker from splashes of blood and specimen during the patient-care
activities. It includes the laboratory gown or coat, face mask, and
gloves. These items are removed at the anteroom or before leaving
the room of the patient in an aseptic, sterile, and pathogen-free way to
avoid contamination. Nowadays, disposable PPE is also available in
the market,
Donning of PPE
1. Gown should be put on first. Make sure that it is fastened, and the
belt is tied.
2, Mask should cover both the nose and mouth.
3. Gloves should be pulled over the gown cuff
Doffing of PPE
1. Gloves are removed first. The contaminated areas should not be
touched with ungloved hands.
2. Gown should be pulled from the shoulders towards the hand so it is
turned inside out
3. Mask should be removed by only touching the string.
Note: Wash hands immediately ater removal of the PPE.
Adltional information oa sae
‘When te colored porton ofthe mask is exposed, you
ate protecting yourset from infection. ve
‘white portion Is the one exposed then hi
you are protecting others from boing i so |
‘you mightbe acariorofadsease.‘i Infection Control, Safety, First Aid, and Personal Wellness 21
Nursery and Neonatal ICU Infection-control Technique
Since newborns are most susceptible to infections because of their
underdeveloped immune system, anyone who enters the nursery area or
neonatal ICU should follow the infection-control procedure:
1, Proper hand washing procedure should be done before putting
on the PPE.
2. The phlebotomists should only bring items necessary for the
specimen collection.
3. Blood collection tray should be left outside the nursery,
preferably the anteroom.
4. Gloves should be removed; hands should be washed; and a new
pair of gloves should be used between patients.
‘Standard and Transmission-based Precaution
for Blood-borne Pathogens
In the clinical laboratory, health workers may be exposed to blood-
borne pathogens (BBP) by needlestick or other injuries caused by
sharp objects. BBP are microorganisms in the human blood that are
infectious and can cause diseases. The most common pathogens include
hepatitis B (HBV), hepatitis C (HCV), and human immunodeficiency
virus (HIV).
Table 2.2 Defense, Hazards, and Symptoms of Biood-borne Pathogens
eee Se
Hepatitis 8 | HEV Blood and other body fulds | Futke fatgve, loss of
Virus (H8V) | vaccination | - Can sunive @ week on objects | aPpette, mid fover, muscle/
“Transmitted via neeciesticks, | jeiabdominal pain, nausea,
senualconact | vomiting
Hopattis | None Blood and other body fulds | Fuk fatigue, loss of
Virus Can sunive @ week on objects | appetite, mild fever, muscle/
Tronsmitied via needlestiks, | oinVabdominel pein, nausea,
sexual contact voutmng
[Hepatitis ¢ | None Blood end serum: sometimes | Putte fatigue, loss of
| vius ey save ‘appette, mic! fever, muscte/
| Infection primarily occurs ater | joinvabdominal pain, nausea,
| large and multiple exposures: vomiting
| Tronsmited vaneedestits, |
| |
‘sexual contact22 _Learing Guide for Principles of Mecical Laboratory Science 2
Exposure Control Plan
Healthcare workers are at risk of being exposed to blood-borne
pathogens as they perform their duties. Exposure can occur when the
following happens:
a. A contaminated needle or sharp object pierces the skin of the
health worker
», Body fluid or blood splashes in the eyes, nose, or mouth of the
health worker
¢. Cut, scratch, or abrasion of the health worker has made contact
with blood or body fluid of an infected patient
d. Human bite cuts the skin of the health worker
During BBP exposure, this procedure should be followed:
1, BBP Exposure Caused by Needlestick or Any Sharp Objects
1, Carefully remove the sharp ot foreign object.
2. Wash the site thoroughly with soap and water for at least
30 seconds,
b, BBP Exposure through Mucous Membrane
1. Flash with water or saline for at least 10 minutes.
2. For the eyes, use eyewash station for flushing, if available.
Remember to remove the contact lenses (if there are any) and
make sure to disinfect them.
3. Report the incident to the immediate supervisor so that
evaluation, treatment, and counseling can be provided.
For surface decontamination, use 1:10 bleach solution or other
disinfectants for the specimen collection and processing areas. Remember
to wear gloves and use an absorbent material when cleaning the area
to avoid spreading the spills over a wider area. As a precaution, all
non-reusable items contaminated by blood or other body fluids should be
placed in biohazard waste containers for proper disposal.Infection Control, Safety, First Ald, and Personal Wellness | 23
Hazards, Warning Symbols, Actions to Take if
Incidents Occur, and Laboratory Safety Risks
Biohazard
Biohazard refers to any material that could be harmful to one’s
health. Biosafety is used to prevent and protect clinical laboratorics from
harmful incidents caused by laboratory specimens that are potential
biohazards.
A health worker could be exposed to a biohazard in various ways.
‘These ways are called the biohazard exposure routes.
1, Airborne ~ from splashes and aerosols during centrifuge and
aliquot; patients with airborne diseases.
a. Observe proper handling practices.
'b. Wear PPE properly.
¢. Use safety shield and guards
2. Ingestion — hands are not sanitized before handling food.
‘a. Wash hands frequently.
b, Avoid hand-to-mouth activities.
. Avoid placing items in the mouth.
Figure 2.6 Biohazard Symbol
3. Non-intact skin ~ contamination through breaks or cuts in the
skin,
‘a. Cover skin breaks or cuts with non-permeable bandages
4, Percutaneous - exposure through the skin due to injuries from
needlesticks and other sharp objects.
a. Use needle safety devices.
>. Wear heavy-duty utility gloves when cleaning broken glass.
¢. Never handle broken glass with bare hands.
5. Permucosal - infection through mucous membranes of the
mouth and nose and the conjunctiva of the eyes.
a. Observe proper handling to avoid aerosols and splashes.
», Avoid rubbing and touching the eyes, nose, and mouth.24 Learning Guide for Principles of Medical Laboratory Science 2
Electric Shock
Another potential hazard that a health worker faces in the workplace
is electric shock especially when dealing with electrical equipment.
‘The following actions should be taken if electric shock occurs:
1, Remove the source of electricity using a non-conductive object or
simply turn off the source of electricity.
2. Ask for medical assistance.
3, Start cardiopulmonary resuscitation (CPR) if necessary.
4. Keep the victim warm.
Fire
Fire is another potential work hazard. Regular fire drills should be
conducted so employees know what to do in case of fire. They should also
be familiar with the location of emergency exits and evacuation plans or
routes. The location of fire extinguishers and heavy blankets should be
posted, and the staff should know how to use them.
Fire extinguishers are classified by the type of fire that they are
designed to extinguish. The classification is listed in Table 2.3.
Table 2:3 Five Classes of Fire
This refers to fire caused by ordinary combustible materials such as wood or paper, which
A | requice water of water-based solutions to extinguish. =
p_| This refers to fire caused by flammable iquids and vapors such as paint, ol, grease, and
_gasoline which require blocking oxygen to snuff out.
‘¢__| Thistefersto fre coused by live electrical equipment, which requires the use
‘of non-conducting agents to extinguish.
This refers to fire caused by combustible and reactive metals such as sodium, potassium,
magnesium, and lithium which need powder agents or sand to put out
x __ | Th8stefers to fre caused by cooking oll, grease, or fats with high temperature which
require agents that prevent splashing, cooling, and smothering the fire.
Radiation
Radiation safety is another consideration of the phlebotomists.
Radiation exposure depends on time, distance, and protection or
shielding. The length of time of the exposure and the distance of theInfection Control, Safety. First Aid, and Personal Wellness 25
individual from the source of radiation matter because the effect is
cumulative. The protection worn at the time also has a bearing on the
intensity of exposure. It is a must that a radiation symbol (Figure 2.7) be
posted in areas where radioactive materials are used and kept.
Radiation hazard can be encountered by the phlebotomists when
collecting specimens from patients who have been injected with
radioactive dyes or from the nuclear medicine department or simply
when delivering specimens to the radioimmunoassay sections of the
laboratory.
Chemicals are used as cleaning reagents, in adding preservatives in
urine container (24-hour), or in delivering specimens to the laboratory.
Inappropriate handling can pose a problem not only to the phlebotomists
but also to other health workers.
As a general rule, the phlebotomists should always wear the PPE
when working with chemicals, Chemical clean-up materials should be
properly used in case of chemical spills.
The following practices should NEVER be done:
1. Storing chemicals above eye level
2. Adding water to acid
3. Mixing chemicals indiscriminately
4, Storing chemicals in unlabeled containers
5, Pouring chemicals into used or dirty containers
6. Using chemicals in ways other than their intended uses
The healthcare worker should be informed where the safety showers
and eyewash stations are located, in the event of chemical spill or splash
in the eyes or body. The personnel exposed to chemical spill or splash
should flush the affected part with water for at least 15 minutes and visit
the emergency room (ER) for evaluation.
Symptoms of Shock
Shock is a condition when there is not enough blood that circulates
back to the heart, which results in inadequate supply of oxygen in
the body. It may be caused by hemorrhage, heart attack, trauma, and
drug reactions.
The common symptoms of shock are as follows: pale, cold and
clammy skin; rapid and weak pulse; increased and shallow breathing; and
expressionless face with a blank stare.
Figure 27 Radiation Symbol
Figure 2.8 Safety Shower
and Eyewash Station
Figure 2.9 Performing CPR26 _Leaming Guide for Principles of Medical Laboratory Science 2
First-aid Procedures
When the patient is in shock, perform the following steps as first aid:
1. Keep the airway open.
2. Call for medical assistance.
3. Keep the patient lying down.
4, Control any bleeding or other cause of shock.
5, Keep the patient warm.
Cardiopulmonary Resuscitation (CPR)
Cardiopulmonary resuscitation (CPR) is a procedure done on a
person who is suffering from cardiac arrest. Being able to perform CPR
‘on a patient increases his/her chance of survival.
Performing CPR Properly
1, While the patient is lying on his/her back on a steady surface, kneel
beside the patient and place your hands in the middle of the patient's
chest.
2. Cover the first hand with the heel of the other hand, interlocking the
fingers. The fingers should be raised so they do not touch the ribcage.
3. Lean forward (shoulders directly over the patients chest) and press
down on the chest for about two inches. Release the pressure to allow
the chest to come back up. Do not release your hands. Repeat. Give
30 compressions at a rate of 100 compressions per minute.
4, Tilt the patient’s head and lift the chin to open the airway allowing
the mouth to fall open slightly.
5. Using your hands, pinch the nostrils and support the chin with
the other hand. Take a normal breath, place your mouth over the
patient's, and blow until you see the chest rise.
6. Remove your mouth and watch the chest fall. Repeat steps five and
six once,
7. Replicate the cycle of 30 chest compressions, to be followed by two
rescue breaths. Repeat this cycle until the patient becomes stable and/
or a medical professional has arrived to help.Infection Control, Safety, First Aid, and Personal Wellness 27.
Personal Welln:
Personal wellness begins with personal hygiene, which includes
bathing regularly, using deodorant, brushing one’ teeth after eating,
keeping one’s hair neat and clean, trimming and cleaning one’s
fingernails, and avoiding the use of heavy lotions and colognes.
One should have proper nutrition and have a balanced diet consisting
of vegetables, fruits, and legumes. Getting enough sleep and staying fit by
having a regular exercise routine are also necessary.
It is important to maintain proper posture and to keep one’s back
safe from any injury by using proper techniques and equipment when
lifting heavy objects, One must achieve and maintain work-life balance by
finding time to relax and to relieve oneself of stress from work.
Figure 240 Components
of Personal Wellness
‘Source: Quad IT Solutions, Inc.