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MSC Biotechnology Syllabus

The document outlines the learning outcome based curriculum framework for the M.Sc. Biotechnology program at Central University of Haryana. It discusses the background and introduction of the department, nature and extent of the program, postgraduate attributes, and structure of the masters course across four semesters.

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0% found this document useful (0 votes)

276 views76 pages

MSC Biotechnology Syllabus

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surenderpradhan

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Learning Outcomes based Curriculum Framework

(LOCF)

as per NEP 2020

For M.Sc. Biotechnology

Department of Biotechnology

School of Interdisciplinary and Applied Sciences

Central University of Haryana

Jant-Pali, Mahendergarh-123031, Haryana

Session 2021-2023
Table of Contents
S. No. Features
1 Background
2 Programme Outcomes
3 Programme Specific Outcomes
4 Postgraduate Attributes
5 Structure of Masters Course
6 Learning Outcome Index
7 Semester-wise Courses and Credit Distribution
8 Course-level Learning Outcomes
9 Teaching-Learning Process
10 Blended Learning
11 Assessment and Evaluation
12 Keywords
13 References
14 Appendices
Background

Considering the curricular reforms as instrumental for desired learning outcomes, all the
academic departments of Central University of Haryana made a rigorous attempt to revise the
curriculum of undergraduate and postgraduate programmes in alignment with National
Education Policy-2020 and UGC Quality Mandate for Higher Education Institutions-2021. The
process of revising the curriculum could be prompted with the adoption of “Comprehensive
Roadmap for Implementation of NEP-2020” in 32nd meeting of the Academic Council of the
University held on April 23, 2021. The Roadmap identified the key features of the Policy and
elucidated the Action Plan with well-defined responsibilities and indicative timeline for major
academic reforms.

The process of revamping the curriculum started with the series of webinars and discussions
conducted by the University to orient the teachers about the key features of the Policy, enabling
them to revise the curriculum in sync with the Policy. Proper orientation of the faculty about the
vision and provisions of NEP-2020 made it easier for them to appreciate and incorporate the vital
aspects of the Policy in the revised curriculum focused on ‘creating holistic, thoughtful, creative
and well-rounded individuals equipped with the key 21st century skills’ for the ‘development of
an enlightened, socially conscious, knowledgeable, and skilled nation’.

With NEP-2020 in background, the revised curricula articulate the spirit of the policy by
emphasising upon— integrated approach to learning; innovative pedagogies and assessment
strategies; multidisciplinary and cross-disciplinary education; creative and critical thinking;
ethical and Constitutional values through value-based courses; 21st century capabilities across
the range of disciplines through life skills, entrepreneurial and professional skills; community
and constructive public engagement; social, moral and environmental awareness; Organic Living
and Global Citizenship Education (GCED); holistic, inquiry-based, discovery-based, discussion-
based, and analysis-based learning; exposure to Indian knowledge system, cultural traditions and
classical literature through relevant courses offering ‘Knowledge of India’; fine blend of modern
pedagogies with indigenous and traditional ways of learning; flexibility in course choices;
student-centric participatory learning; imaginative and flexible curricular structures to enable
creative combination of disciplines for study; offering multiple entry and exit points initially in
undergraduate programmes; alignment of Vocational courses with the International Standard
Classification of Occupations maintained by the International Labour Organization; breaking the
silos of disciplines; integration of extra-curricular and curricular aspects; exploring internships
with local industry, businesses, artists and crafts persons; closer collaborations between industry
and higher education institutions for technical , vocational and science programmes; and
formative assessment tools to be aligned with the learning outcomes, capabilities, and
dispositions as specified for each course. In case of UG programmes in Engineering and
Vocational Studies, it was decided that the departments shall incorporate pertinent NEP
recommendations while complying with AICTE, NBA, NSQF, International Standard
Classification of Occupations, Sector Skill Council and other relevant agencies/sources. The
University has also developed consensus on adoption of Blended Learning with 40% component
of online teaching and 60% face to face classes for each programme.

The revised curricula of various programmes could be devised with concerted efforts of the
faculty, Heads of the Departments and Deans of Schools of Study. The draft prepared by each
department was discussed in series of discussion sessions conducted at Department, School and
the University level. The leadership of the University has been a driving force behind the entire
exercise of developing the uniform template and structure for the revised curriculum. The Vice
Chancellor of the University conducted series of meetings with Heads and Deans to deliberate
upon the vital parameters of the revised curriculum to formulate a uniform template featuring
Background, Programme Outcomes, Programme Specific Outcomes, Postgraduate Attributes,
Structure of Masters Course, Learning Outcome Index, Semester-wise Courses and Credit
Distribution, Course-level Learning Outcomes, Teaching-Learning Process, Blended Learning,
Assessment and Evaluation, Keywords, References and Appendices. The experts of various
Boards of Studies and School Boards contributed to a large extent in giving the final shape to the
revised curriculum of each programme.

To ensure the implementation of curricular reforms envisioned in NEP-2020, the University has
decided to implement various provisions in a phased manner. Accordingly, the curriculum may
be reviewed annually.
Introduction of the Department

The Department of Biotechnology at CUH was established in 2015 under the umbrella of School
of Interdisciplinary and Applied Life Sciences (SIAL) with an aim for providing quality
education and performing cutting edge technological research. Currently the Department of
Biotechnology is kept under the School of Interdisciplinary and Applied Sciences. With faculties
form different backgrounds and skillset in modern technologies, the department is aimed at
training students in the field of biotechnology and related subjects by encouraging
interdisciplinary and multidisciplinary approaches. The focus of the department is also to
develop technologies that seek solutions to real life problems related to society. Therefore, the
department provides an opportunity for students seeking training in an advanced course in
Biotechnology in the form of MSc Biotechnology. The programme is of two years duration (four
semesters) and the curriculum is designed to cater the needs of modern research and
development all over the world.

Learning Outcome based Curriculum Framework

The M.Sc. Biotechnology course at the Department of Biotechnology, CUH has been designed
on the basis of learning outcome based curriculum framework (LOCF) motto. The course covers
the fundamental and advanced areas of Biotechnology with a range of core subjects in each
semester. Along with providing the traditional biotechnology knowledge, the course also has
enough scope for inter- and multi-disciplinary subjects in the form of departmental electives.
This course also caters the skill enhancement needs of the students as well as provides
opportunity for collaboration and learning from other disciplines in the form of general elective
courses, and thus enabling the students to broaden their horizon in complementary subjects.
Every semester has a practical course for strengthening their skills in designing and conducting
experiments in the field of Biotechnology. The six-month dissertation in the last semester orients
and prepares the students for research and development in academia and industry.

Nature and extent of the Programme

The M.Sc. Biotechnology programme is of two years duration. Each year is divided into two
semesters. Each semester will be of sixteen weeks duration. The teaching and learning in the
M.Sc. Biotechnology programme will involve theory classes (lectures), tutorials, practical and
dissertation. The curriculum will be taught through formal lectures with the aid of ICT tools like
power-point presentations, audio and video tools and other teaching aids can be used as and
when required. The specialized subjects could be augmented by special lectures from the
eminent experts in the relevant fields, which can be incorporated along with regular teaching.
The latest developments in the field involving emerging technologies could be incorporated in
the form of seminars, workshops, training, conferences etc.

Postgraduate Attributes

On completion of the course, the students are expected to be proficient in the fundamental,
applied and modern areas of =Biotechnology. They are expected to have acquired the skills of
theoretical and practical aspects of different branches of biotechnology; to be able to develop
rationale thinking skills, logical interpretation and analytical skills. Effective communication of
scientific developments to the society at large is very critical attribute expected from the students
of this course. The attributes expected from the post-graduates of M.Sc. Biotechnology
programme are:

PA1- Fundamental and advanced knowledge of biotechnology and its different branches

PA2- Orientation and specialization in at least one specific branch of biotechnology and related
fields

PA3- Proficiency in theoretical and practical aspects of traditional as well as modern tools and
techniques in the fields of biotechnology

PA4- Awareness and sensitization about various societal problems related to biotechnology

PA-5- Effective communication of scientific knowledge and recent developments with the
society

PA-6-Acquiring skills of writing, editing and publication of research findings in reputed journals
and magazines.

PA-7- Acquire skills and training in scientific communications and presentation

PA-8- Ability to design and undertake research projects to solve societal problems

PA-9-Proficiency in ICT technologies for personal, academic and professional purposes

Aims of Masters Degree Programme in Biotechnology

The objective of this course is to provide fundamental and advanced knowledge of

biotechnology and its related subjects.

 To generate competent human resources skilled to contribute towards the sustainable

development of industry, teaching, and research in different areas of Biotechnology.

 To develop a set of interdisciplinary professional skills that will enable the students in
research and development in Biotechnology.

 To bring social, ethical, and professional awareness among the students about various
issues of contemporary practices in biotechnology and related fields.

Qualification Descriptors

Upon successful completion of the course, the students receive a M.Sc. degree in Biotechnology.
Biotechnology postgraduates of this department are expected to branch out into different paths of
seeking advanced research based knowledge, professional employment, or entrepreneurship that
they find fulfilling. They will be able to demonstrate knowledge as well as skills in diverse fields
of Biotechnology. This will provide a foundation, which shall help them to embark on research
careers by attaining doctoral positions in coveted institutions, as well as securing employment in
research projects in industry or institutes. Besides research, they can get suitable teaching
positions in Colleges and Universities as an Assistant Professor after qualifying National
Eligibility Test (NET). It is expected that besides the skills specific to the discipline, the wider
life skills of analysis, logical reasoning, scientific aptitude, communication skills, research and
life ethics, and moral values will be inculcated in the students. The list below provides a synoptic
overview of possible career paths provided by a postgraduate training in Biotechnology:

1. Research

2. Industry

3. Teaching

4. Biotechnology entrepreneurship

5. Administration and Policy Making

6. Scientific Communication

7. Patents and Law

8. Scientific Writing and Editing

9. Document preparation and publication

Programme learning outcomes M.Sc. Biotechnology

After completion of the programme, the students will be able

PLO 1 – To apply the knowledge of basic biotechnology to solve complex problems in the
society

PLO 2 – To identify, formulate, review research literature, analyse, and design experiments and
identify solutions for complex problems using modern tools

PLO 3 – To apply reasoning informed by contextual knowledge to assess societal, health, safety
and the consequent responsibilities relevant to the professional biotechnology practices.

PLO 4 – To apply ethical principles and commit to professional ethics and responsibilities and
norms of the biotechnology practices.

PLO 5 – To function effectively as an individual and as a member or leader in diverse teams and
in inter- and multi-disciplinary settings.

PLO – 6 To communicate effectively on complex biotechnology activities with the biology

community and with society at large, such as, being able to comprehend and write effective
reports and design documentation, make effective presentations, and give and receive clear
instructions.

PLO – 7 To recognize the need for, and have the preparation and ability to engage inindependent
and lifelong learning in the broadest context of technological change.

Programme Outcomes

● Basic and applied knowledge: Interdisciplinary knowledge to find solution for the
complex biological problems
● Problem analysis: Ability to analyse society related/ applied research problem, design
and execute experiments to find relevant solutions
● Advanced Usage of Technology: Apply advanced instrumentation tools, online
resources with an understanding of the troubleshooting and limitations
● Ethics: Commitment towards professional ethics and responsibilities as a social
endeavour to bring harmony with nature
● Lifelong learning: Scientific skills for industrial applications and entrepreneurship

Programme Specific Outcomes

After completion of M.Sc. Biotechnology, the students will be able:

PSO – 1 To understand the basic principles and applications of biotechnology.

PSO – 2 To understand the principles of microbiology, cell biology, biochemistry, molecular

biology, genetics, and molecular biology involved in biotechnology to identify crucial biological
problems.

PSO – 3 To realize the importance of laws and ethics in biotechnological practices and be able
topractice good laboratory practices.

PSO – 4 To handle basic, sophisticated advanced instruments needed in a research laboratory

with ability to design and execute experiments with precision in a logical manner.

PSO – 5 To understand theoretical as well as practical aspects of gene cloning, expression of

recombinant proteins, tissue culture, and transgenic development.

PSO – 6 To understand the basics of statistics and computational methods used in biological
processes.

PSO – 7 To understand the principles and applications of genomics, transcriptomics, and

proteomics, and integrate the knowledge of omics and genetic engineering to address problemsof
healthcare, crop improvement, energy and environment.

PSO – 8 To understand the principles and applications of bioprocess designing, pharmaceutical

biotechnology, and nanotechnology for solving problems of biology and other related sciences.

PSO-9- To launch start-ups and become entrepreneurs for novel biotechnology products and
processes in various industries.

PSO-10-To understand Biosafety measures, Ethical issues and regulatory compliances in the
field of Biotechnologyand effective scientific communication .
Course Structure semester wise
Semester-I (Total credits - 26)
Course code Course title L T P Type of course Credit
SIAS BT 1 1 01 C 2002 Introduction to Biotechnology 2 0 0 Core 2
SIAS BT 1 1 02 C 4004 Principles of Biochemistry 4 0 0 Core 4
SIAS BT 1 1 03 C 4004 Introduction to Microbiology 4 0 0 Core 4
SIAS BT 1 1 04 C 4004 Genetics 4 0 0 Core 4
SIAS BT 1 1 05 C 4004 Analytical Techniques 4 0 0 Core 4
SIAS BT 1 1 06 C 0084 Practical-I 0 0 8 Core 4
Generic Elective Course (to be opted 4 0 0
from other Departments of CUH or
GEC 4
from SWAYAM/NPTEL MOOC
courses)

Semester-II (Total credits - 27)

Course code Course title L T P Type of course Credit
SIAS BT 1 2 01 C 4004 Cell and Molecular Biology 4 0 0 Core 4
SIAS BT 1 2 02 C 4004 Immunology 4 0 0 Core 4
SIAS BT 1 2 03 C 3003 Biosafety, Bioethics and IPR 3 0 0 Core 3
SIAS BT 1 2 04 C 3003 Genetic Engineering 3 0 0 Core 3
SIAS BT 1 2 05 C 2002 Seminar 1 1 0 Core 2
SIAS BT 1 2 06 C 4004 Omics in Biotechnology 4 0 0 Core 4
SIAS BT 1 2 07 C 0084 Practical-II 0 0 8 Core 4
SIAS BT 1 2 01 DCEC 3003 Pharmaceutical Biotechnology# 3 0 0 DCEC 3
SIAS BT 1 2 02 DCEC 3003 Microbial Biotechnology# 3 0 0 DCEC 3
SIAS BT 1 2 03 DCEC 3003 Environmental Biotechnology# 3 0 0 DCEC 3
#
One of the courses will be opted by the student.
Semester-III (Total credits – 27)
Course code Course title L T P Type of course Credit
SIAS BT 1 3 01 C 4004 Biostatistics and Bioinformatics 4 0 0 Core 4
SIAS BT 1 3 02 C 4004 Biophysics and Nano sciences 4 0 0 Core 4
SIAS BT 1 3 03 C 4004 Medical Biotechnology and 4 0 0 Core 4
Diagnostics
SIAS BT 1 3 04 C 4004 Fermentation and Bioprocess 4 0 0
Core 4
Technology
SIAS BT 1 3 05 C 00084 Practical-III 0 0 8 Core 4
SIAS BT 1 3 01 DCEC 3003 Animal Biotechnology# 3 0 0 DCEC 3
SIAS BT 1 3 02 DCEC 3003 Agricultural Biotechnology# 3 0 0 DCEC 3
Generic Elective Course (to be opted 4 0 0
from other Departments of CUH or
GEC 4
from SWAYAM/NPTEL MOOC
courses)
#
One of the courses will be opted by the student.
Semester-IV (Total credits - 20)
Course code Course title Type of course Credit
SIAS BT 1 4 01 SEC 2002 Review Writing, Editing and Presentation Skills Core 2
SIAS BT 1 4 02 SEC 0018 Dissertation Core 18
Total credits of the Program 100
L- Lecture , T- Tutorial P-Practical; C- Core; DCEC - Discipline Centric Elective Course - to be opted by the
student; SEC- Skill Enhancement Course; GEC- Generic Elective Course.
Learning Outcome Index (Core courses*)

CC
CC CC CC CC CC CC CC CC CC CC CC CC
PSO CC CC CC CC CC
-7
-1 -2 -3 -4 -5 -6 -8 -9 -10 -11 -12 -13 -14 -15 -16 -17 -18

PSO-1 √ √ √ √ √ √ √ √ √ √ √

PSO-2 √ √ √ √ √ √ √ √ √ √ √ √ √ √

PSO-3 √ √ √ √ √ √ √ √

PSO-4 √ √ √ √ √ √ √

PSO-5 √ √ √ √ √ √ √ √ √ √ √ √ √

PSO-6 √ √ √ √ √

PSO-7 √ √ √ √ √

PSO-8 √ √ √ √ √ √ √

PSO-9 √ √ √ √ √

PSO-10 √ √ √ √ √ √ √

*Core courses details

Course code Course title

CC1 SIAS BT 1 1 01 C 2002 Introduction to Biotechnology
CC2 SIAS BT 1 1 02 C 4004 Principles of Biochemistry
CC3 SIAS BT 1 1 03 C 4004 Introduction to Microbiology
CC4 SIAS BT 1 1 04 C 4004 Genetics
CC5 SIAS BT 1 1 05 C 4004 Analytical Techniques
CC6 SIAS BT 1 1 06 C 00105 Practical-I
CC7 SIAS BT 1 2 01 C 4004 Cell and Molecular Biology
CC8 SIAS BT 1 2 01 C 4004 Immunology
CC9 SIAS BT 1 2 02 C 3003 Biosafety, Bioethics and IPR
CC10 SIAS BT 1 2 03 C 4004 Genetic Engineering
CC11 SIAS BT 1 2 04 C 2002 Seminar
CC12 SIAS BT 1 2 05 C 4004 Omics in Biotechnology
CC13 SIAS BT 1 2 06 C 00105 Practical-II
CC14 SIAS BT 1 3 01 C 4004 Biostatistics and Bioinformatics
CC15 SIAS BT 1 3 02 C 4004 Biophysics and Nano sciences
CC16 SIAS BT 1 3 03 C 4004 Medical Biotechnology and Diagnostics
CC17 SIAS BT 1 3 05 C 0202 Fermentation and Bioprocess Technology
CC18 SIAS BT 1 3 04 C 00105 Practical-III

Learning outcome index (elective and skill enhancement courses)*

PSO EC-1 EC-2 EC-3 EC-4 EC-5 SEC-1 SEC-2

PSO-1 √ √ √ √ √ √ √

PSO-2 √ √ √ √ √ √ √

PSO-3 √ √ √ √ √ √

PSO-4 √ √ √

PSO-5 √ √ √ √ √ √

PSO-6 √

PSO-7 √ √ √ √ √ √

PSO-8 √ √ √

PSO-9 √ √ √ √ √

PSO-10 √ √

*Details of elective and skill enhancement courses

EC1 SIAS BT 1 2 01 DCEC 4004 Pharmaceutical Biotechnology

EC2 SIAS BT 1 2 02 DCEC 4004 Microbial Biotechnology
EC3 SIAS BT 1 2 03 DCEC 4004 Environmental Biotechnology
EC4 SIAS BT 1 3 01 DCEC 4004 Animal Biotechnology
EC5 SIAS BT 1 3 02 DCEC 4004 Agricultural Biotechnology
SEC1 SIAS BT 1 4 01 SEC 2002 Review Writing, Editing and Presentation Skills
SEC2 SIAS BT 1 4 02 SEC 0018 Dissertation
Teaching Learning Processes

• Lectures

• Discussions

• Simulations

• Role Playing

• Participative Learning

• Interactive Sessions

• Seminars

• Practical based learning

• Research-based Learning/Dissertation or Project Work

• Technology-embedded Learning

• Reverse classroom based learning

Blended Learning

Blended Learning is a pedagogical approach that combines face to-face classroom methods with
computer-mediated activities in the process of teaching and learning. It implies nice blend of
face-to-face and online activities to make the learning processes more interesting and engaging.
It focuses on integration of traditional classroom activities and innovative ICT-enabled
strategies. It emphasizes student-centric learning environment where the teacher is the facilitator
for productive and measurable learning outcomes. It optimizes and compliments the face to face
learning, giving ample freedom and flexibility to the students and teachers to access and explore
the wide range of open-access sources such as video lectures, podcasts, recordings and articles
through digital platforms. It gives freedom and autonomy to the teachers in selection of
appropriate digital platforms, resources and time-slots to complement and supplement face to
face learning. The Blended Learning doesn’t undermine the role of the teacher, rather it gives
him/her an opportunity to explore the unexplored in accordance with the requirements of the
curriculum. Each course will be taught as 60 % offline and 40 % online mode.

Assessment and Evaluation

• Continuous Comprehensive Evaluation at regular after achievement of each Course-level

learning outcome

• Formative Assessment on the basis of activities of a learner throughout the programme

instead of one-time assessment

• Oral Examinations to test presentation and communication skills

• Online examination and evaluation

• Open Book Examination for better understanding and application of the knowledge acquired

• Group Examinations on Problem solving exercises

• Seminar Presentations

• Review of Literature

• Collaborative Assignments

• Practical examination

Continuous assessment of all papers will be broadly carried out in two forms:

1) Continuous Internal Evaluation (CIE): The theoretical courses will be assessed based on
any or all of the following-written tests, assignments, presentations and regularity in the class.
Assessment of the practical courses will be based on any or all of the following- regularity,
practical records, experiments performance, viva etc. The dissertation will be assessed based on
the regular interaction with the supervisor, regular presentation of work, completion of assigned
tasks, thesis submission, viva etc. The internal evaluation will be carried out throughout the term
and will comprise 30% of the final grade. Participation of students in quiz, seminar, workshop,
games, yoga and other extra-curricular activities will be promoted and facilitated by the
department.
2) Term End Assessment/Evaluation (TEE): The theoretical courses will be assessed based on
written exam, which may be subjective, objective for both. This will cover the entire syllabus.
Assessment of the practical courses will be based on performing and/or description of
experiments, maintaining of the practical records, viva etc. The dissertation will be assessed
based on the thesis reported, viva etc. The end of semester examination comprises 70% of the
final grade.

Both internal and Term end evaluations will be in blended mode.

Keywords

• LOCF

• NEP-2020

• Blended Learning

• Face to face (F to F) Learning

• Online Learning

• Programme Outcomes

• Programme Specific Outcomes

• Course-level Learning Outcomes

• Postgraduate Attributes

• Learning Outcome Index

• Formative Assessment and Evaluation

• Comprehensive and Continuous Evaluation

References

• National Education Policy-2020.

https://www.education.gov.in/sites/upload_files/mhrd/files/NEP_Final_English_0.pdf

• The draft subject specific LOCF templates available on UGC website.

https://www.ugc.ac.in/ugc_notices.aspx?id=MjY5OQ==

• Draft Blended Mode of Teaching and Learning: Concept Note available on UGC website.
https://www.ugc.ac.in/pdfnews/6100340_Concept-Note-Blended-Mode-of-Teaching-and-
Learning.pdf
Department of Biotechnology
Master of Science in Biotechnology (Semester - wise Scheme 2021-2023)
Semester-I (Total credits - 26)
Course code Course title L T P Type of course Credit
SIAS BT 1 1 01 C 2002 Introduction to Biotechnology 2 0 0 Core 2
SIAS BT 1 1 02 C 4004 Principles of Biochemistry 4 0 0 Core 4
SIAS BT 1 1 03 C 4004 Introduction to Microbiology 4 0 0 Core 4
SIAS BT 1 1 04 C 4004 Genetics 4 0 0 Core 4
SIAS BT 1 1 05 C 4004 Analytical Techniques 4 0 0 Core 4
SIAS BT 1 1 06 C 0084 Practical-I 0 0 8 Core 4
Generic Elective Course (to be opted 4 0 0
from other Departments of CUH or from GEC 4
SWAYAM/NPTEL MOOC courses)

Semester-II (Total credits - 27)

Total Credits: 100

Semester Credits Total

credits
Core Skill enhancement Elective courses
courses course
DCEC GEC
(For Department (For other
of Biotechnology Department
students) students)
I 18 4 - 4 26
II 20 4 3 - 27
III 16 4 3 4 27
IV - 20 - - 20
Total 44 32 6 8 100

Generic Elective Course: Offered by Department of Biotechnology to students from other

Departments of University.

Semester Type of course Course code Course title Credit

Principles of
I GEC SIAS BT 1 1 01 GE 4004 4
Biotechnology
Basics of
III GEC SIAS BT 1 3 01 GE 4004 4
Bioinformatics
Course Name: Introduction to Biotechnology Course Code: SIAS BT 1 1 01 C 2002

Batch: Programme: Semester L T P Credits Contact Hrs.

:I per Week: 02
2021-2023 M.Sc. 2 0 0 2 Total Hrs.: 30
Biotechnology
Total Evaluation Marks: 50

CIE: 15 Marks Examination Duration: 2 Hrs.

TEE: 35 Marks
Course To provide basic understanding of biotechnology; it’s scope, different branches and
Objectives applications in the agriculture, medicine, industrial, environment and advancement of
biology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Knowledge in the field of basic biotechnology and career opportunities.
CO2: Understanding of major concepts and disciplines related to biotechnology
CO3: Understanding about the scope and applications of biotechnology in different fields.

COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have four sub-parts and
students need to answer any two. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries three and half
marks.
Unit Contents Contact
No. Hrs.
I An overview-: Definition, scope and importance of old and new biotechnology, 8
Historical development and major breakthrough research in Biotechnology; societal
implications and ethical issues in biotechnology. Concepts of recombinant DNA
technology and Gene Cloning.
II An overview of different fields of biotechnology and their applications- plant 7
biotechnology, animal biotechnology, microbial biotechnology, medical
biotechnology, environmental biotechnology, food biotechnology, pharmaceutical
biotechnology, industrial biotechnology and bioinformatics;

III Career options for biotechnology students in India and abroad; formulation and 8
implementation of strategy for a desired career path; list of leading biotechnology
research institutes/universities/industries in India and abroad;
IV An overview of Nanobiotechnology Indian biotechnology industry: status, 7
opportunities and challenges; bio-entrepreneurship and start-ups; funding agencies for
research and developments. Role of biotechnology in pollution control
Suggested Readings:
1. Elements of Biotechnology (4th reprint), P. K. Gupta, Rastogi Publications, 2019-20.
2. Biotechnology-Expanding Horizons, B. D. Singh, Kalyani Publishers, 2015.
3. Biotechnology: Prospects and Applications (2013). Salar, R.K., Gahlawat, S.K., Siwach, P. and
Duhan, J. S., Springer, Germany. ISBN 978-81-322-1682-7.
4. Textbook of Biotechnology, H.K. Das, John Wiley & Sons 2004.
5. Introduction to Biotechnology (4th edition), W. J. Thieman & M. A. Palladino, Pearson
Publications, 2018.
6. History of Modern Biotechnology, A. Fiechter (Ed.), Springer Publishing House, 2000.
Course Name: Principles of Biochemistry Course Code: SIAS BT 1 1 02 C 4004

Batch: Programme: Semester L T P Credit Contact Hrs.

:I s per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To understand structures and functions of bio-molecules
Objectives
 To provide in depth knowledge of metabolic pathways in the living systems
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Comprehensive knowledge of biochemical pathways operating in living systems
CO2: Advanced knowledge of synthesis and catabolism of major biomolecules
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 15
Monosaccharides-structure of aldoses and ketoses, ring structure of sugars,
conformations of sugars, mutarotation, anomers, epimers and enantiomers.
Disaccharides: maltose, lactose and sucrose. Polysaccharides: homo and hetero-
polysaccharides, structural and storage polysaccharides. Glycolysis - a universal
pathway, reactions of glycolysis, production of acetyl CoA, reactions of citric acid
cycle. Gluconeogenesis, glycogenesis and glycogenolysis.
II 15
Building blocks of lipids - fatty acids, glycerol, ceramide. Storage lipids - triacyl
glycerol and waxes. Structural lipids in membranes-phospholipids,
glycerophospholipids, galactolipids, sulpholipids, sphingolipids and sterols. β-
oxidation of fatty acids. Fatty acid synthase complex. Synthesis of fatty acids.
III 15
Amino acids and peptides- classification (essential and non-essential amino acids),
chemical reactions and physical properties. Introduction to protein structure and
function. Enzymes: classification, kinetics (significance of k m, kcat and Vmax),
inhibition; amino acid metabolism-amino acid deamination and transamination, urea
cycle. Synthesis and utilization of ketone bodies. Biosynthesis and breakdown of
nutritionally non-essential amino acids. Synthesis of other amino acid derivatives
such as neurotransmitters.
IV 15
Nucleotides - structure and properties. Nucleic acid structure-Watson - Crick Model
of DNA. Structure of major species of RNA - mRNA, tRNA and rRNA. De novo
synthesis of purine and pyrimidine nucleotides. Catabolism of purine and
pyrimidine. Disorders of purine and pyrimidine metabolism. Recent advances and
applications in the field.
Suggested Readings:
th
1. Lehninger: Principles of Biochemistry (2017) 7 ed., Nelson, DL and Cox, MM, WH Freeman and
Company (New York), ISBN: 978-1319108243.
th
2. Biochemistry (2017) 6 ed., Garrett RH and Grisham CM, Brooks/Cole, ISBN: 9781305577206.
th
3. Harper’s Illustrated Biochemistry (2018) 7 ed., Rodwell VW, Bender DA, Botham KM,
Kennelly, PJ and Weil PA, McGraw-Hill, ISBN: 9781259837937.
th
4. Lippincott’s Illustrated Reviews Biochemistry (2017) 7 ed., Ferrier, Wolters Kluwer India Pvt.
Ltd., ISBN: 978-9351297949.
th
5. Biochemistry (2019) 9 ed., Stryer L, Berg JM, Tymoczko JL, Gatto Jr. GJ, W.H. Freeman and
Company, New York, USA. ISBN-10: 1-319-11467-9.
Course Name: Introduction to Microbiology Course Code: SIAS BT 1 1 02 C 4004

Batch: Programme: Semester L T P Credit Contact Hrs.

:I s per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
To provide an understanding of basic concepts and techniques in the field of
Objectives
Microbiology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Demonstrate the practical skills in basic microbiological techniques.
CO2: Designate the role of microorganisms in different ecosystems and in various
industries.
CO3: Acquiring knowledge on peculiar features of different microorganisms.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I th 15
History of development of Microbiology in 20 century; Golden era of Microbiology,
Major scientists and their discoveries, The spontaneous generation theory and its
controversy; Germ theory of disease; Methods in microbiology: Physical and
chemical methods of sterilization; Pure culture techniques, Staining techniques,
maintenance and preservation of microbial cultures.
II 15
Binomial nomenclature; Haeckel’s three kingdom classification; Organization of
archae, bacteria and eukaryotic cell; Use of DNA and r-RNA sequencing in
classification of microorganisms; Woese’s three kingdom classification system and its
utility - archaea, eubacteria, eukarya; Different groups of acellular microorganisms -
viruses, viriods and prions.
III 15
General features of microorganisms - bacteria, algae, fungi and protozoa; Bacterial
growth and metabolism; Microbes in different environment: extreme environment,
deep ocean, space and air. Special features of the thermophilic, methanogenic and
halophilic bacteria; Photosynthetic bacteria, Cyanobacteria.
IV 15
Scope of Microbiology - Cycle of matter in nature; Microbial interactions –
Symbiosis and parasitism; Biodegradation and Bioremediation; Biofilms; Microbes in
composting; Biofertilizers and Biopesticides; Microbes and Industry - SCP, microbial
enzymes and fermented foods, Vaccines and antibiotics. Recent advances and
applications in the field.
Suggested Readings:
rd
1. An Introduction to Microbiology (2019), 3 ed., Tauro P, Kapoor KK, Yadav KS, and Sequeira
MG. New Age International Publishers. ISBN: 0852268785.
2. Brock Biology of Microorganisms (2018), 15thed., Madigan MT, Martinko JM, Bender KS, Buckley
DH, Stahl DA Pearson Education, ISBN 9781292235103.
3. Prescott's Microbiology (2017). 10th ed. Sherwood LM, Woolverton C.J McGraw-Hill Education.
ISBN 9781259281594.
4. A text book of Microbiology (2013), 3rd ed. Dubey, R.C. and Maheswari, D.K. Revised S. Chand
and Company Ltd, New Delhi. ISBN: 9788121926201.
5. Microbiology (2001) 5th ed., Pelczar Jr. M, McGraw Hill Education ISBN: 9780074623206.
Course Name: Genetics Course Code: SIAS BT 1 1 04 C 4004

Batch: Programme: Semester L T P Credit Contact Hrs.

:I s per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To study the fundamental concepts of genetics and its role in unification of different
Objectives disciplines of biology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding of basic concepts of classical genetics and genetic analysis of
eukaryotes.
CO2: Acquiring knowledge about the genetic differences in prokaryotes and eukaryotes
CO3: Understanding the effect of different environmental factors on gene expression

COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 15
Historical background, Principles of Mendelian inheritance, detailed discussion on
Mendel’s findings, molecular basis of single gene inheritance, codominance,
incomplete dominance, Gene interactions, interactions of genes in genetic pathways,
pleiotropy, Extra chromosomal inheritance: Maternal inheritance (mitochondria and
chloroplast), Sex linked inheritance, Sex influenced and Sex limited traits, lethal
genes, and multiple alleles, polygenic inheritance, complementation test. Numericals
based on Mendelian inheritance and gene interactions.
II 15
Linkage: complete and incomplete linkage, linkage analysis and genetic maps,
Linkage and recombination of gene, Mechanism of crossing over, Genetic analysis:
calculating recombination frequencies, Linkage maps, types of molecular markers,
mapping with molecular markers, Gene mapping by three point test cross, Tetrad
analysis, Sex determination and Dosage compensation in Mammals and Drosophila,
Quantitative Genetics: Multilocus control; QTL analysis; Quantitative inheritance in
plants and human. Population Genetics and Hardy-Weinberg equilibrium. Genetic
maps, Physical maps. Numericals based on linkage analysis and population genetics.
III 15
Mutations: concept and types, Mechanism of spontaneous mutations, Physical and
chemical mutagenesis, Selection and enrichment of mutants, Molecular mechanism of
induced and spontaneous mutations, importance of mutation; detection of mutation
and directed mutagenesis, types of mutations: insertion, deletion, duplication,
translocation, transposition, frame-shift, mis-sense, non-sense, regulatory region
mutations. Consequences of mutations. Replica plating experiment, Luria and
Delbruck test, Numerical alterations of chromosomes: Ploidy and their genetic
implications.
IV 15
Structure and organization of prokaryotic and eukaryotic and organellar genomes,
Chromatin structure and organisation: nucleosomes, Structure of centromere and
telomere, Euchromatin and heterochromatin, Polytene and lamp brush chromosomes,
Chromatin remodelling, histone modifications, epigenetic inheritance. Gene transfer
in prokaryotes: Transformation, Conjugation and Transduction, Transposons: types,
structures and role in gene regulation, Natural and artificial competence, Operon
concept in bacteria, positive and negative gene regulation, Lytic and lysogenic cell
cycles in Phages, genetic switch in lambda phage. Recent advances and applications
in the field.
Suggested Readings:
th
1. Principles of Genetics (2006) 8 ed. Gardner EJ, Simmons, MJ and Snustad DP, John Wiley &
Sons Inc, ISBN: 8126510439.
th
2. Essentials of Genetics (2015) 9 ed. William S, Michael K, Cummings R, Spencer, CA and
Palladino MA, Prentice Hall Internationals, ISBN-10: 0134047796
th
3. Genetics (2017) 9 ed. Daniel L. Hartal&B. Cochrane, ISBN: 128412293X
4. Introduction to Quantitative Genetics (1995) Falconer DS, and Mackay TFC, ISBN: 0582243025.
5. An Introduction to Population Genetics Theory and applications (2013) Nielsen R and Slatkin M,
Oxford University Press, ISBN: 1605351539.
th
6. Evolution 4 ed. (2017) D. Futuma and M. Kirkpatrick, ISBN: 9781605356051
7. An Introduction to Genetic Analysis (2015) Griffith AJFJ, Wessler SR, Carroll SV and Doebley J,
ISBN: 0-7167-3520-2.
Course Name: Analytical Techniques Course Code: SIAS BT 1 1 05 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

:I per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide an advanced understanding of the core principles and applications of various
Objectives techniques used in biology/biotechnology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Demonstrate principles of various basic and advanced techniques used in biological
experiments.
CO2: Critically analyze and interpret the results obtained from biological experiments.
CO3: Understanding of solving biological problems using various techniques
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 15
Principle of microscopy: resolving powers of different microscopes, magnification;
different types of microscopes, principle and applications of compound microscopy,
dark microscopy, fluorescence microscopy, phase contrast microscopy, confocal
microscopy, atomic force microscopy and electron microscopy (SEM, TEM, STEM);
fixation and staining, freeze fracture/etch techniques.
II 15
Agarose gel electrophoresis, polyacrylamide gel electrophoresis (native PAGE and
SDS-PAGE); Western transfer technique, iso-electric focusing (IEF), 2-Dimensional
gel electrophoresis, pulse field electrophoresis; principle and applications of
centrifugation, differential centrifugation, density gradient centrifugation and
ultracentrifugation; principle and applications of flow cytometry.
III 15
Paper chromatography: types, principles and applications; principle and applications
of thin layer chromatography (TLC), column chromatography (gel filtration, ion
exchange and affinity chromatography); Adsorption and hydrophobic interaction
chromatography, metal chelate chromatography, Structure, principle and applications
of high performance liquid chromatography (HPLC), fast protein liquid
chromatography (FPLC) and gas chromatography (GC).
IV 15
Principle and instrumentation of UV-visible, infrared spectroscopy, atomic absorption
spectrophotometery, NMR spectroscopy, X-ray diffraction spectroscopy, N-terminal
sequencing and peptide synthesis, introduction to proteomics, Yeast 2- hybrid and 3-
hybrid systems, EMSA, CHIP, foot printing, phage display, principle of mass
spectrometry, electrospray ionization MS, MALDI, tandem MS for protein
identification. Recent advances and applications in the field.
Suggested Readings:
th
1. Principles and Techniques of Biochemistry and Molecular Biology (2018) 8 ed. Wilson K and
Walker J, Cambridge University Press, ISBN No. 131661476X.
2. Physical Biochemistry: Principles and Applications (2010) 2nd ed., Sheehan, D., Wiley Blackwell
(West Sussex), ISBN: 978-0-470-85602-4 / ISBN: 978-0-470-85603-1.
3. Physical Biochemistry: Applications to Biochemistry and Molecular Biology (1982) 2nd ed.,
Freifelder D, W.H. Freeman and Company (New York), ISBN:0-7167-1315-2 / ISBN:0-7167-
1444-2.
Course Name: Practical-I Course Code: SIAS BT 1 1 06 C 0084

Batch: Programme: Semester L T P Credits Contact Hrs.

:I per Week:8
2021-2023 M.Sc. 0 0 8 4 Total Hrs.:120
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To inculcate/impart skills to perform various tests/assays and experiments.
Objectives
 To impart skills of experiment designing, practical execution and report writing.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved skills to perform various tests/assays and experiments.
CO2: Improved skills of experiment designing, practical execution and report writing.
CO3: Ability to check the presence of adulterants in various food/feed samples
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 1. Laboratory orientation, calibration, and demonstration of equipments. 30
2. Preparation of solutions, pH and buffers
3. Determination of pKa of acetic acid and glycine
4. Qualitative tests for carbohydrates, lipids, amino acids, and proteins in food
samples
5. Metaphase chromosome preparation with G banding and C banding from blood
sample
6. Preparation of standard curve for quantitative analysis of protein/sugar samples
using calorimetric methods
II 1. Inheritance patterns in man – numerical on pedigree analysis- autosomal patterns, 30
X–linked patterns, Y–linked patterns, mitochondrial inheritance patterns
2. To study numerical based problems on Hardy-Weinberg equilibrium
3. To study numerical problems on linkage mapping
4. To study microscopic examination of bacteria, actinomycetes, algae, fungi and
protozoa with or without staining
5. To study cell counting and cell viability assay
III 1. Preparation of specific media for isolation of bacteria, and fungi from natural 30
sources
2. Production and analysis of microbial enzymes
3. Separation and purification of microbial enzymes/metabolites using various
techniques
4. Biochemical characterization of microbial enzymes/metabolites.
5. To study the thermal denaturation/renaturation profile of DNA sample
IV 1. Separation of carbohydrates, amino acids and plant pigments using paper/thin 30
layer chromatography
2. Isolation of genomic DNA from microbial/plants/other sources
3. Agarose gel electrophoresis for given DNA samples
4. Separation of proteins using native- and SDS-polyacrylamide gel electrophoresis
5. Analysis of protein/DNA samples using UV-VIS spectrophotometer
Suggested Readings:
1. An Introduction to Practical Biochemistry (2017) 3rd ed., Plummer, D.T., McGraw Hill Education,
ISBN: 978-0070994874.
2. Principles and Techniques of Biochemistry and Molecular Biology (2018) 8 th ed. Wilson K, and
Walker J, Cambridge University Press. ISBN: 131661476X.
3. Microbes in Action: A Laboratory Manual of Microbiology (1990) 4 th Addition, Harry W, Seeley,
Paul JV, John J, W. H. Freeman ISBN: 978-0716721000.
4. Genetics: A Laboratory Manual, (2009) 2nd ed., American Society of Agronomy; Lab Manual
edition, ISBN: 978-0891185611.
5. Infant, Child and Adolescent Nutrition: A Practical Handbook (2013) 1 st ed., More J, CRC Press,
ISBN: 9781444111859.
6. Laboratory Manual of Microbiology and Biotechnology (2014) 1 sted.Aneja KR, Scientific
International Pvt., Ltd. ISBN: 9789381714553.
7. Microbiology: A Laboratory Manual (2020), 12th ed., Cappuccino, JH, Welsh CT., Pearson
Education Inc, ISBN: 9780135203996.
8. An introduction to Practical Biochemistry (2017) 3rd ed., Plummer, DT, McGraw Hill Education,
ISBN: 978-0070994874.
Course Name: Principles of Biotechnology Course Code: SIAS BT 1 1 01 GEC 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

:I per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide basic understanding of biotechnology; it’s scope and applications in the
Objectives agriculture, medicine, industrial, environment and advancement of biology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Knowledge in the field of basic biotechnology.
CO2: Understanding of major concepts in the field of biotechnology
CO3: Understanding about the scope and applications of biotechnology in different fields.

IV 15
An Overview, Insights and intervention into the Nano world, Important
Developments, Societal implications & Ethical issues in Nanotechnology,
applications of Nano-biotechnology in different areas. Biotechnology for developing
countries and IPR
Suggested Readings:
1. Lehninger Principles of Biochemistry (2017) 7th ed., D.L. Nelson, M.M. Cox.. W.H. Freeman and
Company, New York, USA, ISBN: 1-4641-2611-9.
2. Microbiology- Concepts and Applications, (1993) 6 th ed., Pelczar MJ et. al., McGraw-Hill Inc, US,
ISBN: 0070492581.
3. Plant Biotechnology – The genetic manipulation of plants (2017) 3 rd ed., Slater A, Scott N and
Fowler M, Oxford University Press, ISBN: 1138407674.
4. Animal Cell Culture Methods In: Methods in Cell Biology, (1998) Volume 57, 1 st ed., Jenni P.M.
and David B., Academic Press, eBook ISBN: 9780080859552.
5. Genome-4, (2017) Brown TA, Garland science, Taylor & Francis, NewYork, ISBN:
9780815345084.
6. Diagnostic and Therapeutic Antibodies (Methods in Molecular Medicine) George AJT, and
Catherine EU, Humana Press, ISBN: 978-0-89603-798-4.
Course Code:
Course Name: Cell and Molecular Biology SIAS BT 1 2 01 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To understand cellular organization and function at molecular level.
Objectives
 To provide understanding of key cellular processes of replication, transcription and
translation.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Advanced understanding of fundamental concepts of cellular and sub-cellular
organization.
CO2: Improved understanding of molecular basis of genetic information and function.

Suggested Readings:
th
1. The Cell: A Molecular Approach (2018) 8 ed., Cooper, GM, Sinauer Associates is an imprint of
Oxford University Press, ISBN: 1605357073.
th
2. Molecular Cell Biology (2016) 8 ed., Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D
and Darnell J, W.H. Freeman &Company (New York), ISBN: 978-1-4641-0981-2 / ISBN:10:
1464183392.
th
3. Molecular Biology of the Cell (2008) 6 ed., Alberts B, Johnson A. Lewis J and Enlarge M,
Garland Science (Princeton), ISBN: 0-8153-1619-4 / ISBN:0-8153-1620-8.
th
4. Lehninger Principles of Biochemistry (2017) 7 ed., Nelson DL, Cox MM, W.H. Freeman and
Company, New York, USA. ISBN-10: 1-4641-2611-9.
th
5. Biochemistry (2019) 9 ed., Stryer L, Berg JM, Tymoczko JL, Gatto, Jr. GJ, W.H. Freeman and
Company, New York, USA. ISBN-10: 1-319-11467-9
6. Genes XII, (2017) 12th Revised edition ed., Lewin B, Krebs J, Kilpatrick ST, Goldstein ES, Jones
and Bartlett Publishers, Inc. Sudbury, Massachusetts, USA. ISBN No. 9781284104493.
7. Molecular Biology of the Gene (2013) 7th ed., Watson JD, Baker TA, Bell SP, Gann A, M, Levin
RL and Cumming B, San Francisco, ISBN: 0321905377.
Course Name: Immunology Course Code: SIAS BT 1 2 02 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To understand overall organization of the immune system and to comprehend the cellular
Objectives and molecular basis of immune responsiveness.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Advanced understanding of the working mechanisms of the immune system.
CO2: Understanding of antibody, MHC, complement system, cytokines, hypersensitivity
and immunobiology of organ transplant.
CO3: Understanding the role of antibody/antigen in disease diagnosis

Suggested Readings:
1. Kuby Immunology (2018) 8th ed., Punt J, Stranford S, Jones P and Owen JA, W.H Freeman and
Company, ISBN: 978-1319114701.
2. Janeway’s Immunobiology (2017) 9th ed., Murphy KM and Beaver C, WW Norton and Company,
ISBN: 978-0815345510.
3. Roitt’s Essential Immunology (2017) 13th ed., Delvis PJ, Martin SJ, Burton DR and Roitt, IM,
Wiley-Blackwell, ISBN: 978-1118415771.
4. Lehninger: Principles of Biochemistry (2017) 7th ed., Nelson, DL and Cox, MM, WH Freeman and
Company (New York), ISBN: 978-1319108243.
5. Lippincott’s illustrated Reviews Immunology (2012) 2nd ed., Doan T, Melvold R, Viselli S and
Waltenbaugh, C, Wolters Kluwer India Pvt, Ltd, ISBN: 978-8184737639.
Course Name: Biosafety, Bioethics and IPR Course Code: SIAS BT 1 2 03 C 3003

Batch: Programme: Semester L T P Credit Contact Hrs.

: II s per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To introduce the concept of intellectual property rights, patenting and emphasis on
Objectives biosafety and bioethics.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding the basics of intellectual property rights.
CO2: Understanding the importance and level of biosafety at laboratory and industrial
levels.
CO3: Understanding the ethical practices and concepts appropriate to the discipline.

COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 12
Biosafety: introduction; historical background; introduction to biological safety
cabinets; primary containment for biohazards; biosafety levels; GRAS organisms,
biosafety levels of specific microorganisms; recommended biosafety levels for
infectious agents and infected animals; definition of GMOs & LMOs; principles of
safety assessment of transgenic plants – sequential steps in risk assessment; concepts
of familiarity and substantial equivalence; risk – environmental risk assessment and
food and feed safety assessment; problem formulation – protection goals, compilation
of relevant information, risk characterization and development of analysis plan; risk
assessment of transgenic crops vs cisgenic plants or products derived from RNAi,
genome editing tools. Regulations: International regulations-Cartagena protocol,
OECD consensus documents and Codex Alimentarius; Indian regulations-EPA act
and rules, guidance documents, regulatory framework-RCGM, GEAC, IBSC and
other regulatory bodies.
II 12
Bioethics: Introduction, ethical conflicts in biological sciences-interference with
nature, bioethics in health care - patient confidentiality, informed consent, euthanasia,
artificial reproductive technologies, prenatal diagnosis, genetic screening, gene
therapy, transplantation. Bioethics in research - cloning and stem cell research,
Human and animal experimentation, animal rights/welfare, Agricultural
biotechnology-Genetically engineered food, environmental risk, labeling and public
opinion. Sharing benefits and protecting future generations - Protection of
environment and biodiversity - biopiracy.
III 10
Patenting: Basics of patents: types of patents; Indian Patent Act 1970; recent
amendments; WIPO Treaties; Budapest Treaty; Patent Cooperation Treaty (PCT) and
implications; procedure for filing a PCT application; role of a Country Patent Office;
filing of a patent application; precautions before patenting-disclosure/non-disclosure -
patent application forms and guidelines including those of National Bio-diversity
Authority (NBA) and other regulatory bodies, fee structure, time frames; types of
patent applications: provisional and complete specifications, PCT and conventional
patent applications.
IV 11
International patenting-requirement, procedures and costs; financial assistance for
patenting, introduction to existing schemes; publication of patents-gazette of India,
status in Europe and US; patent infringement- meaning, scope, litigation, case studies
and examples; commercialization of patented innovations; licensing-outright sale,
licensing, royalty; patenting by research students and scientists-
university/organizational rules in India and abroad, collaborative research-backward
and forward IP; benefit/Credits sharing among parties/community, commercial
(financial) and non-commercial incentives. Recent advances and applications in the
field.
Suggested Readings:
1. Office of the Controller General of Patents, Design & Trademarks; Department of Industrial Policy
& Promotion; Ministry of Commerce & Industry; Government of India. http://www.ipindia.nic.in/
2. World Trade Organisation. http://www.wto.org
3. World Intellectual Property Organisation. http://www.wipo.int
4. International Union for the Protection of New Varieties of Plants. http://www.upov.int
5. National Portal of India. http://www.archive.india.gov.in
6. IPR, Biosafety and Bioethics (2013) Parashar S, Goel D, Pearson Publishing India, ISBN:
9788131774700.
7. An Introduction to Ethical, Safety and Intellectual Property Rights Issues in Biotechnology (2017)
Nambisan P, Academic Press, ISBN: 9780128092316.
8. http://dbtindia.gov.in/guidelines-biosafety
Course Name: Genetic Engineering Course Code: SIAS BT 1 2 04 C 3003

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide basic and high throughput techniques in the areas of genomics and genetic
Objectives engineering.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding of high throughput techniques used in genomics and transcriptomics.
CO2: To understand the concept of genetic engineering including the techniques,
applications and limitations.
CO3: Demonstrate the ability of designing recombinant molecules and conducting
experiments involving genetic manipulation.

IV 12
Applications of genetic engineering in gene therapy, diagnostics, therapeutics, vaccines,
bio-pharma, crop improvement. DNA fingerprinting and its applications in forensics.
Applications of transgenic animals and plants. Regulatory guidelines about transgenic
organisms and their use. Recent advances and applications in the field.

Suggested Readings:
1. Principles of Gene Manipulation and Genomics (2016) 8 th ed., Primrose, SB and Twyman, R,
Wiley Blackwell, ISBN: 978-1405156660.
2. Gene Cloning and DNA Analysis: An Introduction (201978-6) 7 th ed., Brown, TA, Wiley
Blackwell, ISBN: 978-1119072560.
3. Genome 4 (2017) 4th Brown, TA, Garland science, ISBN 13: 978-0815345084.
4. Introduction to Genomics (2015) 2nd ed., Lesk, AM, Oxford university Press India, ISBN: 978-
0198745891.
5. Genomics and Personalized Medicine: What Everyone needs to Know (2016) 1 st ed., Snyder, M,
OUP-USA, ISBN: 978-0190234768.
Course Name: Seminar Course Code: SIAS BT 1 2 05 C 2002

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 02
2021-2023 M.Sc. 2 0 0 2 Total Hrs.: 30
Biotechnology
Total Evaluation Marks: 50

CIE: 15 Marks Examination Duration: 1.5 Hrs.

TEE: 35 Marks
Course To develop communication and presentation skills in the students
Objectives
Course CO1: Students will be able to improve their presentations skills.
Outcomes: CO2: Understanding of scientific papers and their presentations in their own way.

COURSE SYLLABUS
Students will be evaluated by all the faculty members including Head of the Department based on their
presentation followed by viva-voce examination
Unit Contents Contact
No. Hrs.
I Seminar will be of 45-minute duration during which the presentation will be 30
followed by questions session by the audience comprising of faculty and students.
Every student shall be required to submit the topic of his/her seminar in
consultation with the Head of the Department/Faculty members/student advisors
well in advance so that the same may be displayed on the notice board. The
presenter has to write an Abstract to be distributed during Seminar in addition to
two copies of write-up giving relevant details of the background of the subject,
methods used and references/List of sources from where the material for
presentation has been collected.
Suggested Readings:
1. www.pubmed.com
2. www.google.com
3. www.sciencedirect.com
4. www.tandfonline.com
Course Name: Omics in Biotechnology Course Code: SIAS BT 1 2 06 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To provide detailed understanding of major Omics technologies such as genomics,
Objectives
transcriptomics, proteomics and metabolomics etc.
 To provide knowledge about the data analysis of next generation sequencing.
 To provide understanding of applications of the Omics technologies.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding of modern Omics technologies in the field of biotechnology.
CO2: Understanding of data analysis generated through next generation sequencing.
CO3: Better understanding of the applications of the Omics technologies in different
industries.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 15
Introduction to omics techniques, Applications of omics in medical, agriculture, and
other branches of Biotechnology, History of DNA sequencing, Early methods of
DNA sequencing, Genome sequencing projects (Haemophilus, Drosophila, Yeast,
Human etc.), Next Generation Sequencing, Comparative features of different
sequencing platforms (Sanger, Illumina, Nanopore, PacBio etc.), Use of NGS
techniques in genomics and transcriptomics, Basic steps in library preparation and
analysis of genomics and transcriptomics data. Comparative and genomics,
transcriptomics, Functional genomics.

II 15
Introduction to file formats used in NGS analysis, Types of genomics data e.g.
WGS, WES, RNAseq, DNA-methylation, single cell sequencing data etc., Concepts
of sequencing depth, coverage, phred score, N50, L50, and other metrics used in
omics, Introduction to tools and databases used for omics analysis (FastQC, Bowtie,
Stringtie, Tophat, Deseq etc.), Various pipelines for genomics data, Differential
expression analysis, Gene Ontology, Pathway Mapping, Types of non-coding RNAs
and use of high throughput sequencing methods for the analysis of non-coding
RNAs, Applications of genomics and transcriptomics in marker development and
candidate gene discovery.

III 15
Introduction to proteomics, Discovery vs targeted proteomics, Basic techniques for
protein separation and analysis (Chromatography, Gel-based, Spectroscopic), Gel-
based and gel-free techniques in proteomics, Basic workflows and analysis pipelines
(identification, quantification, post-translational modifications etc.), Introduction to
tools used in proteomic analysis (Mascot, Proteome discoverer, MaxQuant etc.),
Applications of proteomics in drug discovery, biomarker discovery, agriculture
biotechnology etc.

IV 15
Introduction to metabolomics and lipidomics, Targeted vs non-targeted
metabolomics, Basics tools and techniques used for metabolome and lipidome
characterization and analysis, Introduction to databases and software used for
analysis of metabolomics data, methods of metabolite identification and
fingerprinting, Applications of metabolomics in medical and agriculture
biotechnology, Integration of different omic techniques for various applications in
biotechnology. Recent advances and applications in the field.

Suggested Readings:
1. Bioinformatics for omics data: methods and protocols (2011), Mayer, B., New York: Humana
Press. ISBN 978-1617790270
2. Omics: Applications in Biomedical, Agricultural, and Environmental Sciences (2013), Barh D.,
Zambare V., Azevedo V. CRC Press. Taylor and Francis Group. ISBN 9781138074750
3. Applications of Advances Omics Technologies: from Genes to Metabolites (2014), Wilson and
Wilsons. Elsevier. ISBN: 9780444626509
4. Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods (2015), Bagchi
D., Swaroop A., Bagchi M. Wiley Blackwell. ISBN:9781118930427
5. Principles of Proteomics (2013), Twyman, R., Garland Science, ISBN: 978-0815344728
Course Name: Practical-II Course Code: SIAS BT 1 2 07 C 0084

Batch: Programme: Semester L T P Credits Contact Hrs.

: II per Week: 8
2021-2023 M.Sc. 0 0 8 4 Total Hrs.:120
Biotechnology
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

II 30
1. Restriction analysis and DNA finger printing methods, RAPD, SSR etc.
2. To study the extraction of RNA from given sample
3. To study introduction to basic linux commands used in omics analyses.
4. To demonstration of NGS pipeline using publically available data for
transcriptome analysis.
5. Identification of non-coding RNAs – a demonstration of pipeline.

45
III
1. To study the preparation of competent cell using chemical method
2. To study the transformation efficiency of competent cells

3. To study the isolation of plasmid DNA from recombinant cell and its analysis
4. To study PCR reactions with plasmid and genomic DNA
5. To study restriction digestion analysis of a given DNA sample
IV 1. To study market analysis of biopharmaceutical industries in India 30
2. To study the demonstration of LC-MS and other proteomics tools and
techniques
3. To study the demonstration of data acquisition in LC-MS techniques
4. To study data analysis using MASCOT or any other software

5. To study dye decolorization using microbial/plant biomass or metabolites

Suggested Readings:
1. Physical Biochemistry: Principles and Applications (2010) 2nd ed., Sheehan, D, Wiley Blackwell (West
Sussex), ISBN: 978-0-470-85602-4 / ISBN: 978-0-470-85603-1.
2. An Introduction to Practical Biochemistry (2017) 3rd ed., Plummer, D.T., McGraw Hill Education,
ISBN: 978-0070994874.
3. Principles and Techniques of Biochemistry and Molecular Biology (2018) 8th ed. Wilson K, and
Walker J, Cambridge University Press. ISBN: 131661476X.
4. Microbes in Action: A Laboratory Manual of Microbiology (1990) 4th Addition, Harry W, Seeley, Paul
JV, John J, W. H. Freeman ISBN: 978-0716721000.
5. Genetics: A Laboratory Manual, (2009) 2nd ed., American Society of Agronomy; Lab Manual edition,
ISBN: 978-0891185611.

46
Course Name: Pharmaceutical Biotechnology Course Code:
SIAS BT 1 2 01 DCEC 3003
Batch: Programme: Semester L T P Credits Contact Hrs.
: per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology II
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To gain insights into the process of drug discovery.
Objectives
 To provide understanding on the mechanisms of various biopharmaceutical
products.
 To gain knowledge on the approval process of biopharmaceuticals.
 To provide knowledge on the market of biopharmaceuticals.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved understanding on the roles of biopharmaceuticals in the treatment of
diseases.
CO2: Understanding of the regulatory mechanism for the approval of biopharmaceuticals.
CO3: Improved insights into the products and market of biopharmaceuticals.

II Biotechnology in pharmaceutical industry: Major areas for biotechnology in the 11

pharmaceutical industry such as antibiotics, vaccines, diagnostics, antibodies,

47
biopharmaceuticals (insulin, interferon, GSF, CSF & therapeutic proteins etc.);
Commercial aspects, priorities for future biotechnological research
III 11
Industrial enzymes in drug development: Penicillin amidase, lipase, oxidoreductase,
nitrilase, protease etc. Use of all these enzymes for enantioselective synthesis of
pharmaceutically important drugs / drug intermediates, future directions.
Approved follow-on proteins/Biosimilars; Characteristics of high-selling peptides
and proteins; Products with expired patents; Challenging originator‟s patents;
Target products for FOB (follow-on biologicals)/ Biosimilars development peptides;
Recombinant nonglycosylated proteins; Recombinant glycosylated proteins;
Industries dealing with biogenerics and its market value; World scenario; Indian
scenario.

IV Genomics in target discovery: Concept of genome, genes and gene expression, 11

genome sequencing and sequence comparison methods (e.g. BLAST), gene
expression comparison methods (microarray). Comparative genomics and
expression genomics for target discovery of communicable diseases and lifestyle
disease. Recent advances and applications in the field.

Suggested Readings:
1. Pharmaceutical Biotechnology (2016) Helmer E, Syrawood Publishing House, ISBN: 978-
1682861066.
2. Pharmaceutical Biotechnology (2014) Sreenivasulu V, Jayaveera KN and Adinarayana K, S Chand
& Company, ISBN: 978-8121942478.
3. Pharmaceutical Biotechnology Fundamentals and Application (2013) Kokare C, Nirali Prakashan,
Educational Publishers, ISBN: 978-8185790688.
4. Pharmaceutical Biotechnology: Concepts and Applications (2011) Walsh G, Wiley India Pvt Ltd,
ISBN: 978-8126530250.
nd
5. Pharmaceutical Biotechnology (2002) 2 ed. Cromelin DJA and Sindelar RD, Taylor and Francis
Group, ISBN: 978-3-527-65125-2.

48
Course Name: Microbial Biotechnology Course Code: SIAS BT 1 2 02 DCEC 3003

Batch: Programme: Semester L T P Credit Contact Hrs.

: s per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology II
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To introduce students to basic and advanced knowledge in the field of microbial
Objectives technology and fermentation for use in human welfare.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding microbial fermentation for the production of useful products
CO2: Understanding basic techniques related to downstream processing of alcohols,
enzymes and organic acids
CO3: Understanding basic techniques related to downstream processing of alcohols,
enzymes and organic acids.

II Fermentative production of citric acid, uses, microorganism, inoculum preparation, 11

medium preparation, fermentation, recovery and mechanism of citric acid production.
Fermentative production of vitamin B12 – Uses, structure of vit-B12,
microorganisms, inoculums preparation, medium preparation, fermentation and
recovery. Fermentative production of glutamic acid – Uses, microorganism, inoculum
preparation, production medium, fermentation and downstream processing

49
III Antibiotics – Commercial production of benzyl penicillin, uses, microorganism, 11
inoculums preparation, production medium, fermentation, recovery and semi-
synthetic penicillins. Fermentative production of tetracyclines-uses, chlortetracycline,
oxy-tetracycline, tetracycline and semisynthetic tetracyclines, structures,
microorganisms, inoculum preparation, production medium, fermentation and
recovery methods.

IV Production and application of microbial enzymes. – Amylases and proteases, uses, 11

microorganisms, inoculum preparation, production medium, fermentation and
recovery, steroid transformations-substrates, typical structures, microorganisms,
inoculum preparation,11-hydroxylation, process and recovery. Principles of vaccine
production and types of vaccines, Microbial biopesticides, microbial products from
genetically modified organisms eg. insulin. Recent advances and applications in the
field.

Suggested Readings:
st
1. Microbial Biotechnology: Progress and Trends (2017) 1 ed., Harzevili FD and Chen H, CRC
Press; ISBN: 978-1138748699.
2. Microbial Biotechnology (2016) Cooper E, Syrawood Publishing House, ISBN: 978-1682860977.
3. Encyclopedia of Metagenomics. Genes, Genomes and Metagenomes: Basics, Methods, Databases
and Tools (2015). Nelson, KE Boston, MA, Springer US, ISBN: 978-1-4899-7479-2.
nd
4. Microbial Biotechnology: Principles and Applications. Hackensack, (2013). 2 ed. Lee, YK,
World Scientific. ISBN: 978-981-256-676-8.
rd
5. Comprehensive Biotechnology (2011) 3 ed., Moo-Young, M, Elsevier, ISBN: 9780444640468.

50
Course Name: Environmental Biotechnology Course Code: SIAS BT 1 2 03 DCEC 3003

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology II
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide information about various factors responsible for environmental pollution and
Objectives its mitigation using biotechnology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding the source and mechanism of environmental pollution.
CO2: Understanding the role of microbes and plants in remediation and management of
environmental pollution.
CO3: Understanding the replacement/options available for non-degradable pollutants.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I Water, Soil and Air pollutants: their sources and effects. Major pollutants and their 12
effects on flora and fauna, Bioremediation-mechanism and process, Factors affecting
the bioremediation process, Natural and engineered bioremediation, concepts of
bioaugmentation, biostimulation, biodegradation, biosorption and biofilms in the
bioremediation of pollutants, In-situ and ex-situ bioremediation strategies.
Phytoremediation, microbial systems for heavy metal accumulation, biosorption &
detoxification mechanisms.

II Primary, secondary and tertiary treatment of waste water, naerobic and aerobic process 11
of treatment; biochemistry and microbiology of aerobic and anaerobic treatment, use of
genetically engineered organisms. Emerging biotechnological processes in wastewater
treatment, Bioremediation of contaminated ground water; Membrane technology in
waste water treatment, Bioreactors for waste water treatment, treatment of typical
industrial effluents: dairy, distillery, dye, and pharmaceutical industries.

51
III 11
Solid waste treatment, characteristics of municipal, industrial and biomedical wastes;
Aerobic and anaerobic methods, Physical and chemical treatment of solid waste,
Composting and vermicomposting. Use of bacteria, fungi, plants, enzymes and
genetically engineered organisms; Bioremediation of contaminated soils and waste
land. Phytoremediation of soil metals; Concept of stubble burning and pollution,
Wealth generation from solid agricultural residues in terms of biofuels and other value-
added products. production of biogas from solid waste.

IV Xenobiotic compounds: Degradation of aliphatic, aromatics, polyaromatic 11

hydrocarbons, polycyclic aromatic compounds, pesticides, microbial treatment of oil
pollution. Enzymes-types and role in biodegradation of pollutants, advantages &
disadvantages of biocatalysts - isolated enzymes versus whole cell systemsImmobilized
biocatalysts in bioremediation. Use of solar radiation in industrial effluent treatment;
Environment friendly technologies and products: biosurfactants, biofertilizers,
biopesticides, integrated waste management. Recent advances and applications in the
field.
Suggested Readings:
th
1. Environmental Science and Technology, (2019) 9 ed., Stankey EM, Lewis Publishers, New York.
ISBN: 1420059203.
st
2. Enviornmental Biotechnology: Principles and Applications (2017) 1 ed., Rittmann B and
Mccarty P, McGraw Hill Education; ISBN: 978-1259002885.
3. Environmental Biotechnology: Biodegradation, Bioremediation, and Bioconversion of Xenobiotics
for Sustainable Development (2016) 1st ed., Sangeetha J, Thangadurai D, David M and Abdullah
MA, Apple Academic Press; ISBN: 978-1771883627.
nd
4. Environmental Biotechnology: Basic Concepts and Applications (2011) 2 ed., Thakur IS, I K
International Publishing House Pvt. Ltd; ISBN: 978-9380578477.
5. Biodegradation and Bioremediation: (2004), Singh A. and Ward O.P., Soil Biology, Springer,
ISBN: 978-3-540-21101-3.

52
Course Name: Biostatistics and Bioinformatics Course Code: SIAS BT 1 3 01 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To introduce the students in the field of bioinformatics and enables them to understand
Objectives the concepts of statistics in biology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding the statistical analysis of biological data
CO2: Understanding the role of computer science in predicting structure and function
of biomolecules
CO3: Understanding similarities and differences among living organisms on the basis
of genetic information

53
coefficient, regression concept and applications.

III 15
Historical background. Scope of bioinformatics - genomics, proteomics, computer
aided drug design (structure based and ligand based approaches), Applications of
bioinformatics. Introduction to biological databases - primary, secondary and
composite databases, Different formats of molecular biology data. NCBI, nucleic
acid databases (GenBank, EMBL, DDBJ, NDB), protein databases (PIR, Swiss-Prot,
TrEMBL, PDB)
IV Similarity, identity and homology. Alignment-local and global alignment, pairwise 15
and multiple sequence alignments, alignment algorithms, amino acid substitution
matrices (PAM and BLOSUM), BLAST and CLUSTAL omega. Identification of
open reading frames (ORF), Concept of orthology, paralogy and homology in gene
and protein sequences. Methods and tools for phylogenetic analysis, maximum
parsimony, maximum likelihood and distance methods; creation, evaluation and
interpretation of evolutionary trees phylogenetic tree. Human Genome Project,
Genome sequencing platforms, Format & Types of genomic data, Software &
pipelines for NGS data analysis. Recent advances and applications in the field.
Suggested Readings:
1. Fundamentals of Statistics (2016) Goon, AM, Gupta, MK and Dasgupta, B. Vol. I & II. World
Press, ASIN: B01LB7MH74.
st
2. Statistical Methods (2012) 1 ed., Das, NG. Vol I & II. Tata McGraw Hill, ISBN: 9780070263512.
th
3. Probability and Statistics for Engineers and Scientists (2013) 9 ed., Walpole, RE, Myers, RH,
Myers, SL and Ye, Pearson Education India KE ISBN: 978-9332519084
th
4. Biostatistics: A Foundation. for Analysis in the Health Sciences (2012) 10 ed., Daniel, WW and
Cross, CL. John Wiley & Sons, ISBN: 978-1118302798
st
5. Essential Bioinformatics (2006) 1 ed., Xiong J, Cambridge University Press, ISBN 13: 978-
0521600828.
6. Fundamental concepts of Bioinformatics (2003) Krane DE and Raymer ML Pearson, ISBN: 978-
8177587579.
7. An Introduction to Bioinformatics (2017) 1st ed., Knight R, Larsen and Keller Education, ISBN:
978-1635490459.
8. Concepts of Bioinformatics and Genomics ( 2016) 1 st ed., Momand J, McCardy A, Heubah, S and
Warter-Perez N, Oxford University Press, ISBN: 978-0199936991

54
Course Name: Biophysics and Nanosciences Course Code: SIAS BT 1 3 02 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To explore the complexity of living systems with a quantitative physical approach,
Objectives
fundamentals of nanoscale structured materials and also discuss various biomedical
& agricultural applications of different nanomaterials.
 To provide understanding of the key biophysical techniques and their applications
in research and diagnosis.

Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved understanding of the core concepts of biology, chemistry and physics and
how they interconnect in biophysical systems.
CO2: Expansion of knowledge of standard molecular and biophysical techniques to design
experiments in a specific research area.
CO3: Improved understanding of fundamental principles of nanotechnology and synthesis
of different nanomaterials.
CO4: Improved insights on the emerging need of nanotechnology in environment, health;
and safety.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I Introduction to Biophysics and history of Biophysics, main features of quantum 15
theory, Elementary particles and their interactions, mechanism of molecular energy
transfer, Distribution of molecular energy and velocity at equilibrium, Energy of
activation, Different types of forces and stereo-chemical factors responsible for
molecular conformation, defining conformation of a macromolecular chain, complex
array of biomolecular structures found in DNA and proteins due to interactions. Main
methods of studying the structure of proteins and DNA, protein folding pathways,
Levinthal’s paradox, Molten globule, Anfinsen’s experiment, Methods for
investigating folding: Fluorescence spectroscopy, Circular dichroism.

55
Macromolecular interactions, Biophysical methods of interactions: Microcalorimetry
(Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR).
II Basic concepts and laws of thermodynamics, Gibbs free energy, Enthalpy and 15
Entropy, Energetic processes in living organism, Information and Entropy, coupling
of fluxes, Coupling of Chemical Reactions, Redox potential in biological system,
ATP production. Introduction to membrane Biophysics, fundamental role of
biomembranes, interfacial phenomena and membranes, surface and interfacial
tensions, self-assembly of membranes, molecular structure of membranes, Structure
& function of membranes, Nernst equation (based on membrane permeable for a
single kind of ions), Resting membrane potential, Action potential, Biophysics of
synapse, patch clamping/voltage clamp and their applications to the study of
biomacromolecules.
III 15
Overview of Nanotechnology - Historical perspective of integration of biology,
chemistry, and material science. Opportunities and promises of Nanobiotechnology.
Top down and bottom up approaches of synthesis of nanoparticles, synthesis of
nanoparticles by physical, chemical and biological methods; nucleation and growth of
nanosystems, factors affecting synthesis of nanoparticles, Debye-Scherrer method,
particle size determination using UV absorption spectra peaks and photoluminescence
peaks, dynamic light scattering (DLS), SEM. Nanomaterials used in biotechnology-
nanoparticles, carbon nanotubes, quantum dots and nanofibres.
IV 15
Miniaturized Devices-nanotechnology and biomedical devices: Overview of smart
devices for medical field, lab on chip concept, epipen, intelligent pill, wobbling gels.
Nanotechnology and diagnostics and therapy-Nano-Biosensing-biosensors and
nanobiosensors -basics, DNA aptamers for nano-biosensing. Use of nanotechnology
in diagnosis of chronic diseases like diabetes and coronary heart diseases; parasitic
disease like malaria. Nanotechnology in agriculture, food technology & environment:
Insecticides development using nanotechnology and Nanofertilizers, nanotechnology
in food processing, safety & smart packaging, applications of nanotechnology in
water purification and oil spill removal. Recent advances and applications in the field.

Suggested Readings:
st
1. An introduction to Biophysics (2018), 1 ed., Burns, D, Forgotten Books, ISBN: 978-1330860212.
st
2. Biophysics - An Introduction (2014) 1 ed., Cotterill, R, Wiley, ISBN: 978-8126551606.
nd
3. Biophysics: An Introduction (2012) 2 ed., Glazer, Springer, ISBN: 978-3642252112.
st
4. Nanobiotechnology: Concepts, Applications and Perspectives (2012) 1 ed., Niemeyer, CM and
Mirkin, CA, Wiley India Pvt Ltd., ISBN 13: 978-8126538409.
st
5. A Textbook of Nanoscience and Nanotechnology (2017) 1 ed., Pradeep T, McGraw Hill
Education, ISBN: 978-1259007323.

56
Course Name: Medical Biotechnology and Diagnostics Course Code: SIAS BT 1 3 03 C 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide an overview about the genetic diseases and the diagnostic techniques used in
Objectives the medical field.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding the basics of genetic information responsible for disease development
CO2: Understanding the classical and advanced methods used for the diagnosis of various
diseases

57
slot blot assay.

IV PCR in molecular diagnostics; multiplex-PCR, quantitative real time PCR (qRT-PCR) 15

and their applications for diagnosis of disease applications, DNA diagnostic system:
molecular beacons and its variants for their applications in detection, molecular
diagnostics in bacterial detection, rolling circle amplification, application of padlock
and selector probes in molecular medicine, DNA aptamers for nano-biosensing,
diagnostics for point-of-care and resource limited settings, smartphones in medical
diagnostics, rapid diagnostic tests (lateral flow assays), concepts of microfluidics,
BioMEMs in diagnostics. Recent advances and applications in the field.
Suggested Readings:
1. Human Molecular Genetics (2018) Strachan T and Read A, Garland Science publisher, ISBN:
9780815345893.
2. Medical Biotechnology (2013) Glick BR, Patton CL and Delovitch TL, ASM Press, ISBN:
155581705X.
3. Advances in Animal Disease Diagnosis (2021). Gahlawat, SK and Maan, S. CRC Press. ISBN
9780367530518 pp. 1-306.
4. Biotechnology in Medical Sciences (2017) Khan FA, CRC Press; ISBN: 978-1138076792
5. Biomedical Nanotechnology (2005) 1st ed., Malsch, N, CRC Press, ISBN: 978-0824725792.
6. Biosensors and Nanotechnology: Applications in Health Care Diagnostics (2018) 1st ed., Altintas
Z, Wiley-Blackwell, ISBN: 978-1119065012.
7. Biosensors: Essentials (2016) 1st ed., Evtugyn, G, Springer, ISBN: 978-3662509388
8. Nucleic Acids as Molecular Diagnostics (2014) 1st ed., Keller, A, Wiley VCH, ISBN: 978-
3527335565.
9. Lateral Flow Immunoassay (2009) Raphael C. Wong, Harley YT, Humana Press, ISBN: 978-1-
58829-908-6.
10. Medical Biotechnology (2013) Glick BR, Patton CL and Delovitch TL, ASM Press. ISBN10
155581705X.
11. Molecular Diagnosis of Infectious Diseases (Methods in Molecular Medicine) (2004) Decker J,
Reischl U, Humana Press, ISBN: 978-1-59259-679-9.
12. Pharmaceutical Biotechnology: Drug Discovery and Clinical Applications, (2006) Kayser O and
Warzecha H, Wiley-Blackwell, ISBN: 978-3-527-60552.
13. Human Molecular Genetics (2018) Strachan T and Read A, Garland Science publisher, ISBN:
9780815345893.

58
Course Name: Fermentation and Bioprocess Course Code: SIAS BT 1 3 04 C 4004
Technology

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide fundamental concepts of different fermentation strategies and to overcome the
Objectives challenges of the new and emerging areas of biotechnology industry.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding basic concept of fermentation strategies
CO2: Understanding of design and operations of a process for bio-based products
CO3: Understanding the structure, operation and functions of various bioreactors
CO4: Critical analysis and improvement in any bioprocess from market point of view.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I Concept of Fermentation, Different types of fermentations-Batch, Fed-batch and 15
continuous fermentation, An overview of submerged and solid state fermentations.
Factors affecting fermentation; Bioreactor- structure and applications of a laboratory
bioreactor; Different types of bioreactors like - Stirred tank reactor, air-lift, packed
bed, fluidized and bubble column- their structure and applications; Multiphase
bioreactor system.
II Sterilization (medium and air)-thermal death kinetics of microorganisms; aeration, 15
agitation and heat transfer in bioprocess. Microbial substrates, Media formulation and
optimization; Microbial growth and kinetics. Monitoring of Bioprocesses: On line
data analysis for measurement and control of important physicochemical and
biochemical parameters, Computer based data acquisition, Techno-economic
feasibility of bioprocess.

59
III Isolation and characterization of industrially important Microorganisms; Generation 15
of mutant strains for fermentation. Different approaches for strain improvement for
fermentation. Concept of primary and secondary metabolites, Yield coefficient and
efficiency. An overview of important products like antibiotic, biofuel, enzymes, An
overview of recombinant proteins.

IV Biological mixture-composition and separation of different components of biological 15

mixture-filtration, centrifugation, sedimentation, flocculation; Cell disruption;
separation of soluble products: liquid-liquid extraction, precipitation,
chromatographic techniques, reverse osmosis, ultra and micro filtration; Purification
of wild and recombinant proteins, Product polishing-drying; crystallization; storage
and packaging. Recent advances and applications in the field.
Suggested Readings:
1. Bioprocess Engineering: Basic Concepts (2017) 3rd ed. Shuler, ML, and Kargi, F. Pearson Prentice
Hall, ISBN: 0137062702.
rd
2. Principles of Fermentation Technology (2016) 3 ed. Stanbury P, Allan Whitaker, Stephen Hall.
Imprint (Butterworth-Heinemann), ISBN: 9780080999531.
th
3. Biochemical Engineering Fundamentals (2013) 5 reprint J. E. Bailey and Ollis, D. F. McGraw-
Hill Education (India) Pvt Ltd., ISBN: 0070701237.
nd
4. Bioprocess Engineering Principles (2013) 2 ed. Doran, P.M, Academic Press, ISBN: 978-0-12-
220851-5.
5. Bioreactors Analysis and Design (2011) Panda T, Tata McGraw Hill, ISBN: 978-0-07-070424-4.

60
Course Code: SIAS BT 1 3 04 C 0084
Course Name: Practical-III

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 8
2021-2023 M.Sc. 0 0 8 4 Total Hrs.: 120
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To inculcate/impart skills on experiment designing, practical execution and report writing.
Objectives
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved skills on experiment designing, practical execution and report writing.
CO2: Acquiring skills to get information and possible practical solution to a research
problem

61
8. To study clonal multiplication of tobacco by shoot tip culture technique.
9. To study induction of embryogenic callus from rice plants.
II 1. To study database search (GenBank, PDB) using BLAST and Sequence 27
submission protocols.
2. To study the sequence alignments (Pair wise and Multiple), Sequence and
structure prediction
3. To study the construction of phylogenetic tree and prediction
4. To study designing of SSR and SNP markers using in silico tools.
5. To study protein structure modelling and docking
6. To study the next generation sequencing data analysis: using freely available
software & pipelines.To study the denaturation kinetics study of biomolecules
using UV-VIS spectrophotometry

III 33
1. To study the structure and functions of a stirred tank bioreactor.
2. To study the production of metabolites in submerged and solid state
fermentations
3. To determine Volumetric Oxygen Transfer Coefficient (kLa) in fermentation
system by dynamic method/sulphite method.
4. Comparative studies on the kinetics of free and immobilized enzymes/cells.
5. To study the production of biofuel/enzyme using lignocellulosic biomass.
6. Comparative study of batch, fed-batch and continuous fermentations.

IV 1. To demonstrate a aptamer based diagnostic test for a disease 30

2. To study the use of real time PCR in the diagnosis of a disease
3. To study the western blot analysis of the proteins using antibodies
4. To study the calculation of mean, median and mode of the given biological data
5. To determine the significance (p-value) of given biological data set
6. To study the synthesis, characterization and applications of nanoparticles

62
Suggested Readings:
rd
1. An Introduction to Practical Biochemistry (2017) 3 ed., Plummer D. T., Tata McGraw Hill
Education Pvt. Ltd. (New Delhi), ISBN: 978-0070994874.
2. Principles and Techniques of Biochemistry and Molecular Biology (2018) 8th ed. Keith Wilson &
John Walker, Cambridge University Press. ISBN: 131661476X.
th
3. Molecular cloning, A Laboratory Manual Vol. I-III. (2012) 4 ed., Green MR and Sambrook J,
Cold Spring Harbor Laboratory Press.
4. Gene Cloning and DNA Analysis (2010) Brown TA, Wiley-Blackwell publishing.
nd
5. Physical Biochemistry: Principles and Applications (2010) 2 ed., Sheehan D, Wiley Blackwell
(West Sussex), ISBN: 978-0-470-85602-4 / ISBN:978-0-470-85603-1.
6. A laboratory Course in Nanoscience and Nanotechnology (2014) Poinern GEJ, CRC press, ISBN:
978-1482231038.

63
Course Name: Animal Biotechnology Course Code: SIAS BT 1 3 01 DCEC 3003

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course This course is an introduction to the theory, standard practices, and methodologies of
Objectives animal biotechnology.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved understanding of animal tissue culture experiments.
CO2: Understanding of gene transfer and gene manipulation methods.
CO3: Improved understanding oftransgenic animal technology.

64
III 11
Manipulation of animal reproduction and characterization of animal genes
Manipulation of reproduction in animals. Artificial insemination, embryo transfer,
in-vitro fertilization. Embryo transfer in cattle and applications. Somatic cell cloning
- cloning of Dolly. Ethical issues. Production of recombinant vaccines. Probiotics
for disease control.

IV 11
Vectors for gene transfer in animals: retrovirus. Gene constructs-
promoter/enhancer sequences for transgene expression in animals. Selectable
markers for animal cells- thymidine kinase, dihydrofolatereductase, CAT.
Transfection of animal cells- calcium phosphate coprecipitation, electroporation,
lipofection, peptides, direct DNA transfer, viral vectors, microinjection. Methods for
producing transgenic animals- retroviral, microinjection, engineered stem cell.
Targeted gene transfer. Transgene integration and identification methods.
Transgenic and genome edited animals. Ethical issues in transgenesis. Recent
advances and applications in the field.
Suggested Readings:
1. Principles and Techniques of Biochemistry and Molecular Biology (2018) 8th ed. Keith Wilson &
John Walker, Cambridge University Press, ISBN No: 131661476X.
2. Advances in Animal Biotechnology and its Applications. (2018). Gahlawat, S.K., Duhan, J. S.,
Salar, R.K., Siwach, P. and Kumar, S. and Kaur, P. Springer, Germany. pp. 1-401. ISBN978-981-
10-4701-5
th
3. Principles of gene manipulation (2016), 8 ed. Primrose Twyman and Old. Blackwell Science,
ISBN: 1405135441.
4. Animal Biotechnology (2013) Verma A and Singh A, Elsevier, ISBN: 9780124160026.
th
5. Molecular Biotechnology (2009), 4 ed. Glick and Pasternak, ASM Press, ISBN10: 1555814980.
rd
6. Recombinant DNA (2006) 3 ed., Watson JD, Richard M. Meyers, Amy AC, Jan AW, Cold
Spring Harbor Laboratory Press, ISBN: 0716728664

65
66
Course Name: Agricultural Biotechnology Course Code: SIAS BT 1 3 02 DCEC 3003
Batch: Programme: Semester L T P Credits Contact Hrs.
: per Week: 03
2021-2023 M.Sc. 3 0 0 3 Total Hrs.: 45
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course To provide knowledge of standard practices, methodologies and applications of
Objectives biotechnology in agriculture.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding the classical and modern approaches of plant/crop breeding
CO2: Understanding the manipulation of plants for improved traits responsible for stress
tolerance and nutrition fortification.
CO3: Understanding of preservation and protection of plants/crops.
COURSE SYLLABUS
NOTE:
i)Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 12
Plant reproduction, breeding of self-pollinated and cross-pollinated crops,
Conventional methods for crop improvement, in-breeding, heterosis, heritability, gene
actions. Molecular markers: definition, properties, types of molecular markers:
restriction based and PCR based, RFLP, AFLP, SCAR, SSR, CAPS, SNP etc. Marker
Assisted Selection (MAS), screening and validation, trait related markers.
Applications of NGS in plant breeding. Quantitative traits, QTL mapping, association
mapping.
II 11
Plant growth regulators; mode of action, effects on in vitro culture and regeneration;
callus culture, suspension culture- batch and continuous culture, Protoplast culture,
somatic hybridization. micropropagation, Meristem culture, shoot tip culture and
production of virus free plants, somaclonal variations, in-vitro production of haploid
plants – androgenesis and gynogenesis, doubled haploid production through distant
hybridization, in-vitro and in-vivo pollination and fertilization, embryo culture,
embryo rescue, somatic embryogenesis, artificial seeds, germplasm conservation and
cryopreservation.

67
III Development of transgenic plants: Agrobacterium mediated transformation, other 11
methods of gene delivery, development of constructs, reporter genes, selectable
markers etc. Gene pyramiding, development of knock outs, RNAi based gene
silencing, CRISPR-CAS9 technology, biopesticides in agriculture (botanicals and
microbials), integrated pest management, Production and applications of biofertilizers
(bacterial, fungal and algal); Plant secondary metabolites: Control mechanisms and
manipulation of alkaloids and industrial enzymes (Shikimate and PHA pathway),
importance of secondary metabolites in agriculture.
IV Genetic engineering for increasing crop productivity by manipulation of 11
photosynthesis, nitrogen fixation and nutrient uptake, Genetic engineering for biotic
stress tolerance (Insects, fungi, bacteria, viruses, weeds). genetic engineering for
abiotic stress tolerance (drought, flooding, salt and temperature). genetic engineering
for quality improvement of protein, lipids, carbohydrates, vitamins (e.g. Golden Rice)
& mineral nutrients, production of antibody in plants; Plant genetic resources, GATT
& TRIPS, Patenting of biological material, patenting of transgenic organisms and
genes, Plant breeders rights (PBRs) and farmers rights, Concerns about GM crops –
environmental, biosafety and ethics. Recent advances and applications in the field.
Suggested Readings:
rd
1. Introduction to plant Biotechnology (2018) 3 ed., Chawla HS, CRC Press, ASIN: B07LH5S4P3.
2. Applied Biotechnology in Genetic Engineering, Pharmaceuticals and Agriculture (2016) Adam
J,Syrawood Publishing House, ISBN: 978-1682862766.
3. Plant Biotechnology: Recent Advancements and Developments (2017). Gahlawat, S.K., Salar,
R.K., Siwach, P. and Duhan, J. S., Kumar, S. and Kaur, P. Springer, Germany. pp.1-390. ISBN
978-981-10-4732-9.
4. Molecular Markers in Plants (2012), Henry RJ, Wiley-Blackwell. ISBN: 978-0-470-95951-0.
5. Genetic Transformation of Plants-Series: Molecular Methods of Plant Analysis (2013) Vol. 23,
Jackson JF and Linskens HF, Springer, ASIN: B000PY3TJ0.
6. Plant Biotechnology – The genetic manipulation of plants (2017) 3rd ed., Slater A, Scott N and
Fowler M, Oxford University Press. ISBN: 1138407674.
7. Plant Transformation Technologies (2011), 1st ed., Stewart CN and Touraev, A Wiley-Blackwell.
ISBN: 9780813821955

68
Course Name: Course title: Basics of Bioinformatics Course Code: SIAS BT 1 3 01 GEC 4004

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 04
2021-2023 M.Sc. 4 0 0 4 Total Hrs.: 60
Biotechnology III
Total Evaluation Marks: 100

CIE: 30 Marks Examination Duration: 3 Hrs.

TEE: 70 Marks
Course
 To provide detailed understanding of Bioinformatics such as genomics, transcriptomic
Objectives
etc.
 To provide knowledge about the data analysis of next generation sequencing.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Understanding bioinformatics analysis of biological data.
CO2: Improved insights into data analysis of next generation sequencing
CO3: Better understanding of the applications of Bioinformatics in biotechnology industry.
COURSE SYLLABUS
NOTE:
i) Question no. 1 is compulsory and to be set from the entire syllabus. It will have seven sub-parts and
students need to answer any four. Each part carries three and half marks.
ii) Question nos. 2 to 5 are to be set from all four units one from each. Every question will have three sub-
parts and students need to answer any two sub-parts of each question. Each part carries seven marks.
Unit Contents Contact
No. Hrs.
I 15
Historical background. Scope of bioinformatics - genomics, proteomics, computer
aided drug design (structure based and ligand based approaches), Applications of
bioinformatics. Introduction to biological databases - primary, secondary and
composite databases, Different formats of molecular biology data. NCBI, nucleic
acid databases (GenBank, EMBL, DDBJ, NDB), protein databases (PIR, Swiss-
Prot, TrEMBL, PDB).
II Alignment-local and global alignment, pairwise and multiple sequence alignments, 15
alignment algorithms, amino acid substitution matrices (PAM and BLOSUM),
BLAST and CLUSTAL omega. Identification of open reading frames (ORF),
Concept of orthology, paralogy and homology in gene and protein sequences.
Methods and tools for phylogenetic analysis, maximum parsimony, maximum
likelihood and distance methods; creation, evaluation and interpretation of
evolutionary trees phylogenetic tree.

69
III Next Generation Sequencing, Comparative features of different sequencing 15
platforms (Sanger, Illumina, Nanopore, PacBio etc.), Use of NGS techniques in
genomics and transcriptomics, Basic steps in library preparation and analysis of
genomics and transcriptomics data. Comparative and genomics, transcriptomics,
Functional genomics.
IV 15
Introduction to file formats used in NGS analysis, Types of genomics data e.g.
WGS, WES, RNAseq, DNA-methylation, single cell sequencing data etc., Concepts
of sequencing depth, coverage, phred score, N50, L50, and other metrics used in
omics, Introduction to tools and databases used for omics analysis (FastQC, Bowtie,
Stringtie, Tophat, Deseq etc.), Various pipelines for genomics data.

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 02
2021-2023 M.Sc. 2 0 0 2 Total Hrs.: 30
Biotechnology IV
Total Evaluation Marks: 50

CIE: 15 Marks Examination Duration: 1.5 Hrs.

TEE: 35 Marks
Course To inculcate/impart the writing and presentation skills.
Objectives
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved skills on review writing and presentation skills.
CO2: Acquiring skills on collecting literature information to identify a research problem

Unit Contents Contact

No. Hrs.
I This is the compulsory and core course for M.Sc. Biotechnology students. Students 30
will be trained in writing review and/or research paper in this course. Supervisor
allotted to each student will train him/her to get literature information on a particular
topic that may be or may not be related to his/her dissertation. Every student will be
asked to give two presentations on a topic related to his/her dissertation in a month in
the presence of all faculty members of the department. The faculty members will
assess the skills acquired by the student in each presentation and provide suitable
suggestions and comments to improve it further. If a student fails to improve in
his/her skills of presentation, one extra chance should be given to the student.

71
Course Name: Dissertation Course Code: SIAS BT 1 4 02 SEC 0018

Batch: Programme: Semester L T P Credits Contact Hrs.

: per Week: 36
2021-2023 M.Sc. 0 0 18 18 Total Hrs.: 500
Biotechnology IV
Total Evaluation Marks: 450

CIE: 135 Marks Examination Duration: 3 Hrs.

TEE: 315 Marks

Course  To inculcate/impart skills on experiment designing, experiment execution and research
Objectives reporting.
 To provide skills on writing thesis dissertation.
Course After completing this course, student is expected to learn the following:
Outcomes: CO1: Improved skills to design, perform, report and present research outcomes.
CO2: Improved skills in writing the research outcomes in the form of thesis dissertation.
CO3: Acquiring skills to work independently as well as in group in order to solve a
research based problem
No. Contents Contact
Hrs.
I Guidelines for Project File 500
Research experience is as close to a professional problem-solving activity as anything
in the curriculum. It provides exposure to research methodology and an opportunity to
work closely with a faculty guide. It usually requires the use of advanced concepts, a
variety of experimental techniques, and state-of-the-art instrumentation.
Research is genuine exploration of the unknown that leads to new knowledge which
often warrants publication. But whether or not the results of a research project are
publishable, the project should be communicated in the form of a research report
written by the student.
Sufficient time should be allowed for satisfactory completion of reports, taking into
account that initial drafts should be critiqued by the faculty guide and corrected by the
student at each stage. The file is the principal means by which the work carried out will
be assessed and therefore, great care should be taken in its preparation.
In general, the File should be comprehensive and include
 A short account of the activities that were undertaken as part of the project;
 A statement about the extent to which the project has achieved its stated goals.
 A statement about the outcomes of the evaluation and dissemination processes
engaged in as part of the project;
 Any activities planned but not yet completed as part of the project, or as a future
initiative directly resulting from the project;
 Any problems that have arisen that may be useful to document for future reference.

72
 The guidelines and format for dissertation is given below:

II Dissertation Guidelines

1. GENERAL:
The manual is intended to provide broad guidelines to the M.Sc. candidates in the
preparation of the dissertation report. In general, the project report shall report, in an
organized and scholarly fashion an account of original research work of the
candidate leading to the discovery of new facts or techniques or correlation of facts
already known.

2. NUMBER OF COPIES TO BE SUBMITTED:

Students should submit three copies to the Head of the Department concerned on or
before the specified date.
ARRANGEMENT OF CONTENTS OF DISSERTATION:

Dissertation material should be arranged as follows:

1. Cover Page & Title page
2. Declaration (See format below)
3. Certificate
4. Abstract (Hindi and English)
5. Acknowledgements
6. Table of Contents
7. List of Tables (optional)
8. List of Figures (optional)
9. List of Symbols, Abbreviations and Nomenclature (Optional)
10. Chapters
11. References
12. Appendices
13. One page CV
The Tables and Figures shall be introduced in the appropriate places.
PAGE DIMENSIONS AND MARGIN:
The dimensions of the dissertation should be standard A4 size paper may be
used for preparing the copies, standard margin with 1.5 line spacing.

5. MANUSCRIPT PREPARATION:
The general text of thesis shall be typed in font style Times New Roman and
font size 12 with 1.5 spacing. Same quality of paper should be used for the preparation

73
of the entire report/thesis; except figure, photos are shown.
5.1 Cover Page & Title Page - A specimen copy of the Cover page & Title page for
report/thesis are given in Annexure I.
5.2 Certificate-The Bonafide Certificate as per the format shown in Annexure II
5.3 Abstract: Abstract should be an essay type (HINDI and ENGLISH) of narration
not exceeding 500 words outlining the research problem, the methodology used for
tackling it and a summary of the findings, typed in 1.5 line spacing.
5.4 Acknowledgements: The acknowledgements shall be brief and should not exceed
one page. The student’s signature shall be made at the right bottom above his / her
name typed in capitals.
5.5 Table of contents - The table of contents should list all material following it as well
as any material which precedes it. The title page, Bonafide Certificate and
Acknowledgment will not find a place among the items listed in the Table of
Contents but the page numbers in lower case Roman letters are to be accounted for
them. One and a half spacing should be adopted for typing the matter under this
head. A specimen copy of the Table of Contents for report / thesis is given in
Annexure III.
5.6 List of Table - The list should use exactly the same captions as they appear above
the tables in the text and the caption shall follow ‘sentence case’. One and a half
spacing should be adopted for typing the matter under this head.
5.7 List of Figures - The list should use exactly the same captions as they appear below
the figures in the text and the caption shall follow ‘sentence case’. One and a half
spacing should be adopted for typing the matter under this head
5.8 List of Symbols, Abbreviations and Nomenclature - One and a half spacing
should be adopted for typing the matter under this head. Standard symbols,
abbreviations etc. should be used.
5.9 Chapters - The chapters may include
Chapter I – Introduction
Chapter II – Review of Literature
Chapter III –Materials and Methods

Chapter IV- Results and Discussion

Chapter V-Summary and Conclusions
1.10. Research output/outcome if any published or presented in
conference/seminar/symposium may be included.
1.11. List of References - Any works of other researchers, if used either directly or
indirectly, should be indicated at appropriate places in the report/thesis. The
citation may assume any one of the following forms. APA Style.

74
APA in-text citation style uses the author's last name and the year of publication, for
example: (Field, 2005).

Example:
Derwing, T. M., Rossiter, M. J., & Munro, M. J. (2002). Teaching native speakers to
listen to foreign-accented speech. Journal of Multilingual and Multicultural
Development, 23(4): 245-259.
Thomas, H. K. (2004). Training strategies for improving listeners' comprehension of
foreign-accented speech (Doctoral dissertation). University of Colorado, Boulder.
TYPING INSTRUCTIONS
6.1 General
This section includes additional information for final typing of the thesis. Some
information given earlier under 'Manuscript preparation' shall also be referred. The
impressions on the typed/duplicated/printed copies should be black in colour.
Corrections, interlineations and crossing out of letters or words will not be permitted in
any of the copies of the report/thesis intended for submission. Erasures, if made,
should be neatly carried out in all copies. A sub-heading at the bottom of a page must
have at least two full lines below it or else it should be carried over to the next page.
The last word of any page should not be split using a hyphen. One and a half spacing
should be used for typing the general text. The general text shall be typed in Font Style
Times New Roman and Font Size 12 with 1.5 spacing.
Single spacing should be used for typing:
(i) Long Tables
(ii) Long quotations
(iii) Foot notes
(iv) Multilane captions
(v) References

6.2 Chapters The format for typing chapter headings, division headings and sub
division headings shall be same as given in Table of Contents.
7. Binding specification- Thesis should be spiral or soft cover book bound, the cover
of the thesis should be of blue color printed with golden ink and the text for printing
should be identical as prescribed for the title page.

75
Format for Declaration by the candidate
DECLARATION

I ………………………, student of the School of Interdisciplinary and Applied

Sciences, Central University of Haryana, Mahendergarh hereby declare and certify
with my signature that my thesis entitled ………………………………………….
submitted to the Department of Biotechnology, Central University of Haryana, India
in partial fulfillment of the requirements for the award of the Degree of Masters of
Science is a record of original research work done by me and the dissertation has not
been the basis for the award of any degree/diploma/associateship/fellowship or
similar title of any candidate of any University. I have faithfully and accurately cited
all my sources, including books, journals, handouts and unpublished manuscripts, as
well as any other media, such as the Internet, letters or significant personal
communications.

I understand the concept of “plagiarism” and declare that while drafting this
dissertation I have refrained from plagiarism. I know that plagiarism not only
includes direct copying, but also the extensive use of other’s ideas without proper
referencing or acknowledgement (which includes the proper use of references and
quotation marks).

If my dissertation found to be plagiarized at any point of time, I’ll be solely

responsible and will be ready to accept any decision taken by the competent
authority including rejection of my dissertation.

Signature of student

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