SHEEP HUSBANDRY DEPARTMENT (BARAMULLA)
SMALL RUMINANT HOSPITAL AND DISEASE INVESTIGATION LABORATORY
Chijhama , Baramulla (193301)
: +91 7889448766; :
[email protected] LABORATORY EXAMINATION REPORT
Dr.Sai maMushtaq Clinician:Dr.IrfanMd. Magray. Incharge:Ehtesham Rashid.
MVSc (Vet Microbiology) Veterinary Assistant Surgeon Phone number: 7006966594
Laboratory Officer (Baramu lla) Phone number:7006235522 SEC:Watergam
Phonenumber:7889448766 SDB:Kitchama Private Ram: Khalid Khazir.
Ref:DIL/ Bla/2024-25/111 Ref: VAS/ Rafi/2024-25/117 R/o:Marangund.
Date of dispatch: 11/11 / 2024 Dated: 09/ 11/ 2024
LABORATORY SAMPLE DETAILS
Case history/Anamnesis of the animal/s or flock sampled ***
Date of collection of samples 09.11.2024
Date of receipt of samples at DIL Baramu lla 09.11.2024
Total number of samples received 01
Type of samples received Seru m
Type of samples used for laboratory analysis RBPT
Date of testing of laboratory samples 09.11.2024
Any special test/investigation requested by the clinician/owner ***
Sd/=
Laboratory Officer
Disease Investigation Laboratory
Noorbagh Baramu lla
I. Rose Bengal Plate Test
S.No Species Sex Age Conditi on of Conditi on of Test result Ti me of
sample recei ved sample tested reaction (mi n)
1 Ovine Male *** Clear Clear -ve ≥4
II. Anote to the Clinician:
a) The Rose Bengal Plate Test (RBPT), in principle, is essentially a serological variant of plate/slide
agglutination test (visible clump ing observed in ≤4 minutes) in whichkilled Gram negative
bacillinamely Brucellaabortusorganismsstained with acidified Rose Bengal dye (containing 0.95g/ L of
Sodiu m azide as a preservative and buffered at pH=3.6) are used as antigen for direct detection of
Brucella agglutinins / antibodies in the serum of the suspected ruminant animal (A m. J. vet res, 18:
550(1957); J A m Vet Med Assoc: 151: 1778(1967); Co mp . Immune M icrobiol Infect Dis, 1 107
(1978).
b) This Antigen is reactive with both immunoglobulin G (detected earlier especially in subclinical
infections) and immunoglobulin M.
c) It is a quick yet inexpensive and highly effective test for screening of the livestock for Brucellosis,
with a d iagnostic specificity sometimes approaching 100% (OIE, 1995).
d) No test is 100% sensitive and/or specific. RBPT has a tendency to give False Negative results (WHO
Tech. Rep. Ser., 740 (1986) 62-63; J. Clin. Microbiol., 36 (1998) 1425-1427; Proc. Final Res. Coord.
Meet. FAO/IAEA/SIDA, Austria (1990) 151-162) dueto low antibodytitres, observer bias and prozone
phenomenon.
e) Many biochemical conditions such as vaccination with strain-19, Covid-19 infections, Tularaemia
infections, Yersinia enterocoliticaO:9infections, Escherichia coliinfections, Salmonella group N
(O:30) infect ionsand Hyperbilirubinaemia may affect the reaction results; however Hemoglobin levels
and Lipaemic conditions do not seem to be among the interfering factors.
f) Disease Investigation Laboratory Baramulla publishes results keeping in view the probabilit ies of false
positive and false negative results arising due to a plethora of factors as can be imagined under a given
set of field and/or laboratory conditions. The results published by DIL Baramu lla must be carefully
compared and correlated with the pertinent clinical signs and symptoms in the animal/s under
investigation. In case the test results do not seem to be complying with the clinical picture, the animals
can be resampled and retested at DIL Srinagar using ELISA fo r confirmation (J. Bacteriol. Res., 1 (3)
(2009) 30-33; Turk. J. Vet. Anim. Sci., 37 (2013) 1-5) to provide a better clinical understanding of the
investigative process.
III. A note to the Breeder/Owner/Animal Attendant:
a) The RBPT test when performed under standard, controlled conditions can yield highly specific results.
Once the sera samples submitted to the Disease Investigation Laboratory Noorbagh Baramu lla are
declared as showing a Brucellosis positive reaction to the test antigen, the infected animals have to be
disposed off to stop the infection from spreading to other animals and humans However it can also
spread by other routes such as by consuming raw infected milk fro m ru minants, handling infected
animal t issues such as uterine secretions, placentae and fetuses or by entering skin wounds, cuts or
intact mucous memb ranes and handling infected laboratory samp les .
b) In animals it causes poor reproductive performance such as abortions, still births, retention of
placenta, repeat breeding and decreased libido in males.So me animals may recover, parturate normally
and produce offspring folowing treatment with antibiotic drugs. However, they may continue shedding
the Brucellotic bacilli for a pro longed period thereafter.
c) Brucellosis is a zoonotic disease, meaning that it can in fect and clin ically affect humans as
well,causing a febrile disease known as Undulant Feverwherein it causes severe debilitating problems
such as fatigue, irregular fever, headache, asthenia (Weakness, weight loss), profuse sweating (foul
smelling perspiration), chills, migratory arthralgia (sacroilitis, osteomyelitis, spondylodescitis, septic
arthritis, epidural abscess), infertility (Ep ididy mo-Orchit is), ly mphadenopathy, hepatosplenomegaly,
blindness (uveitis, keratoconjunctivitis, iridocyclitis, optic neuritis, cataract) and death (pericardit is,
endocarditis).
Sd/=
Laboratory Officer
Disease Investigation Laboratory
Noorbagh Baramu lla
…….because diagnosis is everything…….
