Practical 2: STI cases 2022 Student sheet page 1
The class will be put into multiple groups of students.
Event 1 Watch the Videos (A) a POCT for Candida, (B) a student video on STI
Event 2 CGs prepare answers to the questions below; contribute on PollEv.
Questions: True or false?
1 In bacterial vaginosis (BV):
a. BV can be diagnosed by PCR
b. Clue cells support the diagnosis
c. The whiff test is positive
d. The pH of vaginal secretions is increased >4.5
e. BV is associated with increased transmission of HIV
2 In a person with a genital ulcer:
a. Consider HPV
b. Do HSV serology
c. Do HSV PCR
d. Do a syphilis EIA
3 In an infection with Chlamydia trachomatis:
a. Culture is more sensitive than PCR
b. Infection in women may be asymptomatic
c. C. trachomatis causes epididymitis in men
d. Urine can be used for a PCR test
4 In gonorrhoea
a. A urethral Gram stain is helpful in men
b. Urine can be used for a PCR test
c. Blood agar is the best agar for culture of vaginal swabs
d. It may be found in rectal and throat samples
5 Mycoplasma genitalium
a. Causes urethritis
b. Is diagnosed by culture
c. Shows resistance to both macrolides and quinolones
d. Can be treated by amoxicillin
6 In a person with an STD
a. Test for HIV
b. Test for HBV
c. Test for syphilis
d. Trace contacts
Clinical ‘STI’ case video 2021 Student sheet page 2
Event 3 Each group solves five clinical cases
Consider the history, and test results to try to establish the diagnosis.
Each group enters their Final diagnosis for their ‘case’ on PollEv when instructed.
Clinical cases
1 22 yr old male gym instructor, with penile discharge & dysuria. An STD screen
shows:
a. Gram stain shows - Gram negative, intracellular diplococci
b. PCR for GC/CT - both positive
c. HSV PCR – not done
d. Candida POCT - not done
e. Whiff test – not done
f. Serology
1. Syphilis – EIA negative
2. HIV - negative
3. HBV SAg - negative
2 A 42 yr old female with a smelly vaginal discharge. An STD screen shows:
a. Gram stain shows - clue cells present
b. PCR for GC/CT - negative
c. HSV PCR - not done
d. Candida POCT - not done
e. Whiff test – positive
f. Serology
i. Syphilis – EIA negative
ii. HIV - negative
iii. HBV SAg - negative
3 A 38 yr old female attends a contact clinic. Her new boyfriend had developed
dysuria. An STD screen shows:
a. Gram stain - not done
b. PCR for GC/CT - positive for CT
c. HSV PCR - not done
d. Candida POCT - not done
e. Whiff test – not done
f. Serology
i. Syphilis – EIA negative
ii. HIV - negative
iii. HBV SAg - negative
4 A 55 yr old male golf player has painful penile blisters; some have ulcerated. An
STD screen shows:
a. Gram stain - not done
b. PCR for GC/CT - negative
c. HSV PCR - positive
d. Candida POCT - not done
e. Whiff test - not done
f. Serology
i. Syphilis - EIA positive
ii. HIV - positive
iii. HBV SAg - positive
5 A 25 yr old female complains of vaginal itchiness and discharge. It irritates on
passing urine. She had a UTI treated last week
a. Gram stain shows - clue cells not seen
b. PCR for GC/CT - negative
c. HSV PCR - negative
d. Candida POCT – ****Students do a POCT****
e. Whiff test - negative
f. Serology
i. Syphilis - negative
ii. HIV - negative
iii. HBV SAg - negative
