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DLC Viva Questions Answers

The document provides a comprehensive overview of Differential Leukocyte Count (DLC), including its definition, normal ranges for different types of white blood cells (WBCs), and the procedure for performing DLC. It also discusses various conditions related to WBC counts, such as leucocytosis and leucopenia, and factors causing changes in specific WBC types. Additionally, it outlines the identification of WBCs under a microscope and precautions for making a DLC smear.

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Chetan Sharma
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674 views2 pages

DLC Viva Questions Answers

The document provides a comprehensive overview of Differential Leukocyte Count (DLC), including its definition, normal ranges for different types of white blood cells (WBCs), and the procedure for performing DLC. It also discusses various conditions related to WBC counts, such as leucocytosis and leucopenia, and factors causing changes in specific WBC types. Additionally, it outlines the identification of WBCs under a microscope and precautions for making a DLC smear.

Uploaded by

Chetan Sharma
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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DLC (Differential Leukocyte Count) Viva Questions with Answers

1. What is DLC?

Answer: Differential leukocyte count is the percentage count of different types of WBCs in a given blood

smear.

2. What is the normal range of each type of WBC?

Answer: Neutrophils: 40-75%

Lymphocytes: 20-45%

Monocytes: 2-10%

Eosinophils: 1-6%

Basophils: 0-1%

3. Why is DLC done?

Answer: To detect infections, allergic reactions, blood disorders, and diagnose leukemia or parasitic

diseases.

4. How is DLC performed?

Answer: By making a Leishman-stained blood smear, observing under microscope, and counting 100 WBCs.

5. Which stain is used in DLC?

Answer: Leishman stain (contains eosin and methylene blue).

6. How to identify different WBCs under microscope?

Answer: Neutrophil: 2-5 lobed nucleus, pink granules

Lymphocyte: Round nucleus, scant cytoplasm

Monocyte: Kidney-shaped nucleus, gray cytoplasm

Eosinophil: Bilobed nucleus, orange granules

Basophil: Large purple granules

7. What is leucocytosis and leucopenia?


DLC (Differential Leukocyte Count) Viva Questions with Answers

Answer: Leucocytosis: Increased WBC count; Leucopenia: Decreased WBC count

8. What causes neutrophilia?

Answer: Bacterial infections, inflammation, MI, stress.

9. What causes lymphocytosis?

Answer: Viral infections, chronic infections, leukemia.

10. What causes eosinophilia?

Answer: Allergies, asthma, parasitic infections, skin diseases.

11. What is shift to left and right in neutrophils?

Answer: Shift to left: Increased immature neutrophils (band cells) in acute infection.

Shift to right: Hypersegmented neutrophils in megaloblastic anemia.

12. What is toxic granulation?

Answer: Dark coarse granules in neutrophils seen in severe infections.

13. In which conditions are monocytes increased?

Answer: TB, typhoid, malaria, subacute bacterial endocarditis.

14. In which conditions are basophils increased?

Answer: CML, polycythemia vera, hypersensitivity reactions.

15. What precautions are taken while making DLC smear?

Answer: Use clean slide, proper angle, air dry quickly, fresh stain, count only mature WBCs.

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