SDS
Safety Data Sheet
1. IDENTIFICATION
Product Identifier: Methylene Blue, 0.05-1% w/v Aqueous
Product Code(s): M-2123, M-2124, M1019, M1023
Synonyms: Basic Blue 9 Solution; 3,7-Bis(dimethylamino)phenazathionium Chloride Solution;
Tetramethylthionine Chloride Solution
Recommended Use: For manufacturing, industrial, and laboratory use only. Use as a laboratory reagent.
Uses Advised Against: Not for food, drug, or household use.
Supplier: The Science Company
7625 W Hampden Ave #14 Lakewood CO 80227
Phone: (303) 777-3777 Fax: (303) 777-3331
Emergency Phone Number: For health emergency, call poison control: (800) 222-1222.
2. HAZARDS IDENTIFICATION
Hazard Classifications: This product is classified as not hazardous under OSHA's Hazard Communication
Standard, 29 CFR 1910.1200 (HCS) and the United Nations’ Globally Harmonized System
of Classification and Labeling of Chemicals (GHS). However, all chemicals handled and
used in the workplace should be treated with caution.
Signal Word: Not applicable.
Hazard Statements: Not applicable.
Pictograms: Not applicable.
Precautionary Statements:
Prevention: Not applicable.
Response: Not applicable.
Storage: Not applicable.
Disposal: Not applicable.
Hazards Not Otherwise Not applicable.
Classified:
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 1/7
Toxicity Statement: This product consists of no greater than 1% ingredients whose acute toxicities are unknown.
3. COMPOSITION AND INFORMATION ON INGREDIENTS
Component Common Name / Synonyms CAS# Chemical Formula % by Weight
Water Water 7732-18-5 H 2O ≥ 99.0
Methylene Blue,
Basic Blue 9 7220-79-3 C16H18N3SCl • 3H2O 0.0500 – 1.00
Trihydrate
Trade Secret Statement: Not applicable.
4. FIRST AID MEASURES
First Aid Procedures:
Inhalation: Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial
respiration. Call a physician if symptoms occur.
Ingestion: Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs,
keep head low so that vomit does not enter lungs. Never give anything by mouth to an
unconscious person. Call a physician or poison control center if symptoms occur.
Skin Contact: Remove contaminated clothing and shoes. Wash skin with soap and plenty of water for at
least 15 minutes. Wash clothing before reuse. Call a physician if symptoms persist.
Eye Contact: Check for and remove contact lenses if present and easy to do. Immediately flush eyes with
gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids
occasionally. Call a physician if symptoms occur.
General Advice: Poison information centers in each state can provide additional assistance for scheduled
poisons. Ensure that medical personnel and those providing first aid are aware of the
material(s) involved and take precautions to protect themselves.
Symptoms and Effects: Irritation, vomiting, diarrhea, cyanosis. May be harmful if swallowed. May cause irritation to
skin, eyes, respiratory tract, and gastrointestinal tract. May affect the stomach and blood.
Immediate Medical Care/ If you feel unwell or are concerned, get medical attention. Treat symptomatically. Symptoms
Special Treatment: may be delayed.
5. FIREFIGHTING MEASURES
Suitable Extinguishing Media: Water spray, dry powder, alcohol resistant foam, carbon dioxide.
Unsuitable Extinguishing Media: Do not use a solid (straight) water stream, as it may scatter and spread fire.
Hazardous Combustion Carbon oxides, nitrogen oxides, sulfur oxides, hydrogen chloride.
Products:
Specific Hazards: Excessive thermal conditions may cause decomposition and yield corrosive and/or toxic
fumes.
Special Protective Equipment/ As in any fire, wear MSHA/NIOSH-approved (or equivalent), self-contained, positive-
Precautions for Firefighters: pressure or pressure-demand breathing apparatus and full protective gear. Use water spray
to cool unopened containers. In the event of fire and/or explosion, do not breathe fumes.
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 2/7
6. ACCIDENTAL RELEASE MEASURES
Personal Precautions and Isolate hazard area and keep unnecessary and unprotected personnel away from the area
Protective Equipment: of leak or spill. Wear appropriate personal protective equipment (see Section 8). Avoid
contact with eyes, skin, and clothing.
Emergency Procedures: In case of chemical emergency, or if unsure how to address an accidental release, consult a
professional (see Section 1).
Methods for Containment: Stop the flow of material, if this is without risk. Prevent entry into waterways, sewer,
basements, or confined areas. Dike the spilled material, where this is possible. Product
should not be released to the environment. Contain and recover liquid when possible.
Methods for Cleanup: Absorb spill with an inert material (e.g. vermiculite, dry sand, earth, cloth, or fleece) and
place in a non-combustible container for reclamation or disposal. Do not flush to sewer.
Clean contaminated surface thoroughly. Residues from spills can be absorbed with acetone
or alcohol. Never return spills in original containers for reuse. Clean up in accordance with
all applicable regulations.
7. HANDLING AND STORAGE
Handling: Wear personal protective equipment (see Section 8). Provide sufficient air exchange and/or
exhaust in work areas. Avoid contact with skin, eyes, and clothing. Do not breathe vapors or
spray mist. Do not ingest. When using, do not eat, drink, or smoke. Keep away from
incompatible materials (see Section 10). Handle in accordance with good industrial hygiene
and safety practice. Wash thoroughly after handling. Containers of this material may be
hazardous when empty, as they retain product residues. Observe all warnings and
precautions listed for this product.
Storage: Store in a cool, dry, ventilated area. Store in a segregated and approved area away from
incompatible materials (see Section 10). Store in original container. Keep containers tightly
closed and upright. Keep away from food, drink, and animal foodstuffs. Keep out of the
reach of children. Comply with all national, state, and local codes pertaining to the storage,
handling, dispensing, and disposal of this product.
8. EXPOSURE CONTROLS AND PERSONAL PROTECTION
Exposure Limits: Water: No information found.
Methylene Blue, Trihydrate: No information found.
Engineering Controls: Ensure adequate ventilation. Ventilation rates should be matched to conditions. If
applicable, use process enclosures, local exhaust ventilation, or other engineering controls
to maintain airborne levels below recommended exposure limits. If exposure limits have not
been established, maintain airborne levels to an acceptable level.
Personal Protective Measures:
Eye/Face Protection: Wear safety glasses with side shields or goggles. Maintain approved eye wash station and
accessible rinse facilities in work area.
Skin Protection: Wear appropriate chemical resistant clothing (with long sleeves) and appropriate chemical
resistant gloves.
Respiratory Protection: An air-purifying, NIOSH-approved respirator with appropriate cartridge or canister may be
permissible under certain circumstances where airborne concentrations are expected to
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 3/7
exceed exposure limits. Use a positive-pressure, air-supplied respirator if there is any
potential for an uncontrolled release, if exposure levels are unknown, or in any other
circumstances where air-purifying respirators may not provide adequate protection.
Specific Requirements Ensure that glove material is compatible with this product. This information is available from
for Personal Protective glove manufacturers.
Equipment:
9. PHYSICAL AND CHEMICAL PROPERTIES
Unless otherwise indicated, all properties are given at 25 °C and standard pressure.
Appearance: Blue, opaque liquid.
Odor: Odorless.
Odor Threshold: No information found.
Formula Weight: Mixture.
pH: No information found.
Melting/Freezing Point: No information found.
Boiling Point/Range: No information found.
Decomposition Temperature: No information found.
Flash Point: Not applicable.
Auto-ignition Temperature: Not applicable.
Flammability: Not flammable.
Flammability/Explosive Limits: Not applicable.
Solubility: Miscible with water.
Vapor Pressure: No information found.
Vapor Density: No information found.
Specific Gravity: 1.00 (Water = 1)
Evaporation Rate: No information found.
Viscosity: No information found.
Partition Coefficient No information found.
(n-octanol/water):
10. STABILITY AND REACTIVITY
Reactivity Data: No information found.
Chemical Stability: Stable under normal conditions.
Conditions to Avoid: Heat, incompatible materials.
Incompatible Materials: Strong oxidizers.
Hazardous Decomposition Carbon oxides, nitrogen oxides, sulfur oxides, hydrogen chloride.
Products:
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 4/7
Possibility of Hazardous May react vigorously or violently if exposed to extreme thermal conditions or in contact with
Reactions: the incompatible materials listed above. Excessive thermal conditions may yield hazardous
decomposition products listed above.
Hazardous Polymerization: Will not occur.
11. TOXICOLOGICAL INFORMATION
Routes of Exposure: Inhalation, ingestion, skin contact, eye contact.
Acute Effects: May be harmful if swallowed. May cause irritation to skin, eyes, respiratory tract, and
gastrointestinal tract. May affect the stomach and blood.
Chronic Effects: No information found.
Toxicological Data: Water: Not applicable.
Methylene Blue, Trihydrate: Irritating to skin and eyes based on animal data.
Symptoms of Exposure: Irritation, vomiting, diarrhea, cyanosis.
Carcinogenic Effects: No component of this product is considered to be a carcinogen by IARC, ACGIH, NTP, or
OSHA.
IARC: Methylene Blue, Trihydrate: 3 - Not classifiable as to its carcinogenicity to humans
12. ECOLOGICAL INFORMATION
Ecotoxicological Data: Water: Not applicable.
Methylene Blue, Trihydrate: No information found.
Persistence and Degradability: No information found.
Environmental Effects: Not expected to be hazardous to the environment. However, the possibility of an
environmental hazard cannot be excluded in the event of unprofessional handling or
disposal.
13. DISPOSAL INFORMATION
Disposal Instructions: All wastes must be handled in accordance with local, state, and federal regulations.
Minimize exposure to product waste (see Section 8).
Contaminated Packaging: Because emptied containers retain product residue, follow label warnings even after
container is emptied. Offer rinsed packaging material to local recycling facilities.
Waste Codes: No information found.
14. TRANSPORT INFORMATION
DOT: Not regulated.
Environmental Hazard No information found.
Regulations:
Other Transport Precautions: No information found.
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 5/7
15. REGULATORY INFORMATION
U.S. Federal Regulations:
OSHA: This product is not considered a "Hazardous Chemical" as defined by the OSHA Hazard
Communication Standard, 29 CFR 1910.1200.
TSCA Inventory: All components of this product are on the U.S. TSCA Inventory.
U.S. EPCRA (SARA Title III):
Section 302: No information found.
Sections 311/312:
Hazard Category List (Yes/No)
Section 311 – Hazardous Chemical No
Immediate Hazard No
Delayed Hazard No
Fire Hazard No
Pressure Hazard No
Reactivity Hazard No
Section 313: No information found.
CERCLA Reportable Quantities: No information found.
International Inventories:
Country or Region Inventory Name On Inventory (Yes/No)*
Australia Australian Inventory of Chemical Substances (AICS) N/A
Canada Domestic Substances List (DSL) N/A
Canada Non-Domestic Substances List (NDSL) N/A
China Inventory of Existing Chemical Substances in China (IECSC) N/A
Europe European Inventory of Existing Commercial Chemical Substances N/A
(EINECS)
Europe European List of Notified Chemical Substances (ELINCS) N/A
Japan Inventory of Existing and New Chemical Substances (ENCS) N/A
Korea Existing Chemicals List (ECL) N/A
Philippines Philippine Inventory of Chemicals and Chemical Substances (PICCS) N/A
*A "Yes" indicates that the listed component(s) of this product comply with the inventory requirements administered by the
governing country(s).
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 6/7
16. OTHER INFORMATION
Disclaimer: The Science Company provides the information in this Safety Data Sheet in the belief that it
is reliable but assumes no responsibility for its completeness or accuracy. The physical
properties reported in this SDS are obtained from literature and do not constitute product
specifications. The Science Company makes and gives no representations or warranties
with respect to the information contained herein or the product to which it refers, whether
express, implied, or statutory, including without limitation, warranties of accuracy,
completeness, merchantability, non-infringement, performance, safety, suitability, stability,
and fitness for a particular purpose. No warranty against infringement of any patent,
copyright or trademark is made or implied. This SDS is intended only as a guide to the
appropriate handling of the material by a properly trained person. It shall be the user's
responsibility to develop proper methods of handling and personal protection based on the
actual conditions of use. Accordingly, The Science Company assumes no liability
whatsoever for the use of or reliance upon this information including results obtained,
incidental or consequential damages, or lost profits.
Issue Date: May 19, 2016
Reason for Revision: Update of Section 9 over 12/02/2015 version.
Product: Methylene Blue, 0.5-1% w/v Aqueous
Revision Date: 05/19/2016 7/7
