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LAF Dan Autoclave - Fix

This document discusses aseptic techniques and principles of sterilization. It defines terms like enteral, parenteral, and contamination. It describes sources of product contamination like people and air supply. It explains laminar air flow hoods which use HEPA filtered air to sweep the work area and prevent contaminated air entry. Hoods are used to prevent contamination of sterile products. The document also discusses autoclaves and the principles of steam sterilization, how steam condenses and draws in more steam to sterilize items. Standard sterilization temperature and pressure is listed as well as sterilization times for different materials.

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Ipung Arisanti
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114 views18 pages

LAF Dan Autoclave - Fix

This document discusses aseptic techniques and principles of sterilization. It defines terms like enteral, parenteral, and contamination. It describes sources of product contamination like people and air supply. It explains laminar air flow hoods which use HEPA filtered air to sweep the work area and prevent contaminated air entry. Hoods are used to prevent contamination of sterile products. The document also discusses autoclaves and the principles of steam sterilization, how steam condenses and draws in more steam to sterilize items. Standard sterilization temperature and pressure is listed as well as sterilization times for different materials.

Uploaded by

Ipung Arisanti
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 18

Theoretical Principles and Practical Skills of

Aseptic Manipulations

Page 1

Definitions
Background
Laminar Air Flow Hoods
Aseptic Technique
Free Powerpoint Templates

Page 2

Definitions

Enteral
Of or relating to the intestines
(e.g. by mouth, orally, per tube)
Parenteral

Page 3

Definitions (cont.)

Contamination any effect or action that has a negative


impact on a product's integrity making it unfit for use
Chemical composition
pH
Sterility (e.g. microorganism contamination)

Page 4

Background

Sources of product contamination


People (most common)
Touch contamination
Generation of particulates from shedding cells or hair
Page 5

Supply air

Laminar Air Flow Hoods

The underlying principle of a laminar air flow


hood is that a constant flow of HEPA filtered air
at a rate of approximately 90 linear feet per
minute physically sweeps the work area and
prevents the entry of contaminated air
The hood workspace is used to prevent the
contamination of compounded sterile products.

Page 6

Laminar Air Flow Hoods (cont.)

HEPA Filter in Frame

Page 7

Laminar Air Flow Hoods (cont.)

Horizontal Flow (Laminar Flow Hood)


Air blows towards worker
Page 8

Laminar Air Flow Hoods (cont.)

The HEPA filter is located in the fragile mesh between


thin metal strips at the back of the hood behind the
HEPA filter screen
Nothing should be permitted to come in contact with the
HEPA filter
NO cleaning solution

Page 9

Laminar Air Flow Hoods (cont.)

No Paper, Pens, Calculators, or Labels in


the Hood
Only products essential to product preparation
should be placed in the laminar flow hood to
minimize the potential for contamination
Page 10

Laminar Air Flow Hoods (cont.)

Eating, drinking, and smoking is always


prohibited
Talking or coughing should be directed away
from the hood to minimize air flow turbulence
A mask covering mouth and nose must be worn

Page 11

Autoclaves
Safe and Effective Use

Page 12

Principles of Steam Sterilization (cont.)

The killing power of steam is due to its latent


heat of vaporization
80 calories: 1 L water to boilingg
540 calories: 1 L boiling water to steam

Page 13

Action of Steam

Steam contacts a cooler surface,


condenses, causing a huge decrease in
volume and setting up a negative
pressure that draws more steam
Condensation occurs as longg as there is
a temperature differential

Page 14

Standard Temperature and Pressure

250 F (121 C) and 15 p.s.i.

Berapa atm ?

How Long to Autoclave


Dry goods @ 121 C

Time (min)

Glassware, empty, inverted

15

Instruments, wrapped

30
Page 16

Special Situations

Media boils out of bottles


differs among media
Very dense or closed dry objects

Page 17

TERIMA
KASIH

Page 18

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