PHYCOSOL INTERNSHIP
ALGAL TECHNOLOGY FOR
WASTE WATER
TREATMENT
By G Sangamithra
Btech Biotechnology
Rajalakshmi Engineering College
Third Year
Internship date: 02/01/2025 to 08/01/2025
DAY 1: ALGAE
• Eukaryotic (except cynaobacteria)
• Versatile Economic advantages Media preparation
• Food source • BG11 (Fresh)
• Plant (under-developed)
• Water purification • BBM (Fresh)
• Autotrophic • Climate change mitigation • F/2 (Marine)
• Photosynthesis Application
• Nutrient supply (food supplement)
• Food colour
• Animal feed
Types of Algae • Cosmetics -due to antioxidant activity
• Oral care –toothpaste- antibacterial
• Based on habitat
activity
Fresh water • Biofuel-high lipid
• Agriculture-manure-pest control
Marine • Bioremediation
• Research
• Based on pigments
Challenges
Chlorophyta
• Scaleup
Rhodophyta • Risk of contamination
DAY 2: WATER
4 parameters to test water quality
Water Chemical
March 22 World water • pH
• 96.5% Ocean daytypes:
Algae • Ion concentration
• Macro • Conductivity
• 1.74% Glacier
• Micro • Dissolved O2 concentration
• 1.64% Ground water Macro Eg Kelp Physical
Micro Eg Spirulina (protein rich), • Temperature
• 0.007% River and lake Cynobacteria (prokaryotic) • Turbidity
• Colour
• 0.001% Atmosphere • Solid %
Biological
• 0.001% Soil Microbiological
• Trace Biological
2.5% Fresh water (68.7% ice, 30.1% Ground water, 1.2% Surface water)
Usage (0nly 0.3%)
• 8% Domestic
• 22% Industrial
ALGAL PHYCOREMEDIATION Future
• Genetic engineering
Use of plants like Hydrilla, Arabidnosis, etc.
• Algal bioreactor
• Integration
• Bioaccumulation
• Climate change mitigation
• Biosorption
To measure the growth of algae
• Biochemical transformation
• OD
• Extracellular polymeric substance • Biomass
• Chlorophyll a, b only in Chlorophyta
• Cell count
• Increase in pH (algae grows in alkaline condition
Challenges
• TDS –should decrease as algae grows
• Scale up • BOD
• COD
• Microbial competition • Conductivity –salinity
• Climate change
Algae’s basic need:
• Optimal growth condition • NKP
• Na2PO4, K2PO4, NH2PO4 etc
• Risk of harmful algal bloom
• CO2 reduces pH (90%)
DAY 3: ALGAE AROUND US
Dutlina salina - carotein- pink pond
• Sambar lake –red colour
• Initially they are green under high salt produces carotein and lipids
Algal cultivation
• Flamingo consumes this algae thus has pink feathers
Open ray pond
Diatoms (bioluminasense) • High volume
• 5-10 lack litre of water
Aztec’s lake (Mexico)
• Central medial with a paddle
• Lake Chad • Height of 20-25 cm for Spirulina
• General height 30-40cm
• Harvest algae • Most prominent method
• Rpm based on algal culture
• Dry them into sheets
• 1:9 parts of inoculum
Some products • Inoculation time early morning or evening
• Paddling from sunrise to sunset
• Nori sheets Bioreactors
• Sea grapes • High cost
• Controlled environment
DAY 4: LAB TO INDUSTRY
• Economy Research and Development
• Job creation (R&D):
• Hypothesis formulation
• Good impact for society • Experimentation
• Data analysis
• Knowledge transfer
• Result discussion
Other:
Key stakeholders
• Feasibility
• Researcher • Trial and error
• Visual effect
• Institute • Patent
• Industry
• Government
Steps:
• Research
• Patent
DAY 5: INDUSTRIAL VISIT
THANK YOU