MEGA (Molecular Evolutionary Genetics Analysis)
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The document discusses the construction of evolutionary trees using MEGA software, which is utilized for statistical analysis of molecular evolution and phylogenetic tree construction. It outlines the various functions of MEGA, including sequence alignment and inferring divergence times, and provides instructions for creating a phylogenetic tree using maximum likelihood methods. The document emphasizes the role of MEGA in understanding evolutionary histories and selective forces impacting gene evolution.
MEGA (Molecular Evolutionary Genetics Analysis)
- 1. Athar Hussain Roll No.2015 BS(Hon) Bioinformatics and Biotechnology Govt.College University Faisalabad Email: [email protected]
- 2. Construction of Evolutionary tree Using MEGA
- 3. MEGA, Molecular Evolutionary Genetics Analysis, is a freely available software for conducting statistical analysis of molecular evolution and for constructing phylogenetic trees. MEGA is an integrated tool for conducting : • Sequence alignment, • Inferring phylogenetic trees, • Estimating divergence times, Introduction
- 4. Conti…….. • Estimating rates of molecular evolution, • Inferring ancestral sequences, • Testing evolutionary hypotheses. MEGA is used by biologists in a large number of laboratories for reconstructing the evolutionary histories of species and inferring the extent and nature of the selective forces shaping the evolution of genes and species
- 6. Creation Of New Alignment
- 7. # Select Types of Sequence to be Align
- 10. Sequence Alignment Using ClustalW
- 14. Export Alignment to MEGA Format
- 15. Save MEGA format File
- 16. Construction Of Phylogenetic Tree Using Maximum Likelihood Method
- 17. Opening MEGA format File which we Saved
- 18. Parameter Setting For Phylogenetic Tree
- 19. Progressing Tree
- 20. Rectangular Phylogenetic Tree Showing the Conserved Residues
- 21. Circular Tree Construction From Rectangular Tree
- 22. Final Circular Phylogenetic Tree After Grouping Genes Different color represents different Groups