Welcome
University of Agricultural Sciences, Dharwad
College of Agriculture, Vijaypur
Properties of asexually propagated crops,
Clonal selection and
Clonal degeneration
Submitted to: Submitted by:
Dr. M B Boranayak Brahmesh Reddy B R
Dept. of genetics & Plant Breeding III yr A sec B1 batch
College of Agriculture,Vijayapur College of Agriculture, Vijayapur
Asexual reproduction
Multiplication of plants without the fusion of male and female
gametes is known asexual reproduction.
Asexual reproduction can occur either
1. By vegetative plant parts or
2. By vegetative embryo which develop without sexual fusion.
Rhizome : Turmeric (Curcuma domestica) Ginger(Zingiber officinale)
Bulb : Garlic (Allium sativum), Onion (A. cepa)
Corm :Bunda (Colocasia antiquorum) Arvi (Colocasia esculenta)
Tuber : Potato (Solanum tuberosum)
Significance
Asexual reproduction has several advantages.
1. It leads to continuity of same genotype with great precision, because
all the progeny have similar genotype and phenotype.
2. Useful in obtain large number of genetically identical individuals.
3. Promising genotype can be maintained.
4. It makes use of desirable bud mutations. Mutants can be directly
released as varieties.
Clone
 Progeny of a single plant
obtained by asexual
reproduction is called a
clone.
 Crop which are propagated
asexually or by vegetative
means clonal crops.
Features of Clones
 Homogeneous constitution :- The progeny of clone is
genetically identical and have same genetic constitution. Thus clones
are homologous. No genetic variation within a clone.
 Heterozygosity :-Asexually propagated crops are heterozygous
so clone is also heterozygous. Progeny looks similar phenotypically
but is heterozygous.
Vigorous growth
Clones have hybrid vigour which is conserved due to sexual
reproduction. Most of the varieties of sugarcane and potato are
hybrids
Source of variation
There are three sources of variation in a clone. Viz. bud mutations,
mechanical mixture, and occasional sexual reproduction.
Segregation in F1
When hybridization is done between different clones, segregation occurs in
F1generation. Each F1 plant is potentially a new variety, therefore, selection
is practised in F1.
Wider adaptation:
Clones are more adaptable to environmental conditions
Origin of Genetic variation
within Clones.
1. Somatic mutation
2. Mechanical mixture
3. Sexual reproduction
4. Segregation in F1
Comparison among Clones, Purelines and Inbreds
Particulars Clone Pureline Inbred
Mode of pollination Cross pollination Self pollination Cross Pollination
Natural mode of reproduction Asexual Sexual Sexual
Genetic makeup of plant population Heterozygous Homozygous Hetrozygous
Obtain through Asexual reproduction
from a single plant
Natural S.P.from a
single
homozygous plant
Artificial self pollination and
selection for several generation
Maintained through Asexual reproduction Natural self
pollination
Artificial self pollination or Close
Inbreeding
Used as a variety Yes Yes No (Used for Hybrid and synthetic
Hybrid development)
Genetic makeup of plant with in an
entity
Heterozygous Homozygous Almost Homozygous
Method of improvement
• A single outstanding plant select from a population forms the
basis of a new variety.
• The breeding behaviour of genotype of this plant is not important
since there would be no further sexual reproduction.
• An improved variety that has becomes variable, or from a
population produces by crossing two or more clones.
Clonal selections
Improvement of asexually propagated crops by
selecting superior clones is known as clonal selection.
Superior clones can be isolated from three types of materials
1. Local variety
2. Introduced variety
3. Inter crossed population
Breeding procedure of clonal
selection
It involves a minimum of 7 years for complete selection of the best
superior and desired clones
SECOND YEAR:-
Clones from the selected plants grown separately.
a) Desirable clones selected
THIRD YEAR :-
Preliminary yield trials with standard checks.
a) Selection for quality, disease resistance etc. Disease
nurseries may be planted.
b) Few outstanding clone selected.
FOURTH-SIXTH YEAR :-
Multilocation yield trials with standard checks.
a) Best clone identified for release as a new
variety.
SEVENTH YEAR :-
The best clone release as a new variety.
a) Seed multiplication for distribution begins.
Clonal selection degeneration
Schematic
representation
of clonal
selection
Merits
1. Variety evolved by this method retains all the characters of the
parental clones for several years.
2. Varieties are highly uniform like pure lines. They are highly
stable because there is no risk of deterioration due to
segregation and recombination
3. Effective method for genetic improvement of asexually
propagated crops.
Merits (contd..)
4.Useful in isolation the best genotype from a
mixed population of asexually propagated crops.
5.The selection scheme is useful for maintaining the
purity of clone.
Demerits:-
1. selection method utilizes the variability already present in the
population, and it has not been devised to generate variability.
2. Genetic makeup cannot be improved by this method without
hybridization.
3. Varieties developed by clonal selection are highly prone to
new of a disease.
Clonal selection degeneration
Clonal Degeneration
The loss in vigour and productivity of clone with time is
known as clonal degeneration.
 Clonal degeneration= Degeneration is inherented in
the clone.
 Degeneration may result from
1) Mutation
2) Viral diseases
3) Bacterial diseases
• Pidi Monthan is a clonal selection from Monthan.
• High Gate is a clonal selection from Mitchel.
• Kurfi Red Potato is a clonal selection from Darjeeling Red Round.
It was propagated from a single disease free plant.
• Kurfi Safed is a clonal selection from potato variety Phulwa.
• Bombay Green Banana is a bud selection from Dwarf
Cavendish.
Major achievements
Achievements in clonal selection:-
Crop
 Mango:-
.
 Grapes:-
 Guava:-
 Sapota:-
Clonal variety
– Dasehari-51, Niranjan, Cardoz mankurad, Payur-1
– Pusa seedless, Thomson seedless, Perlet, Tas –e-
ganesh, Sonaka, Rao sahebi, dilkhus.
– L-49 Jyoti, Alandi, S-1, Arka mridula,
– Cricket ball, Kirthi bharti, Badami, Baramasi, Guthi,
Thagarampudi.
 Papaya:-
 Pineapple:-
 Litchi:-
 Aonla:-
 Bael:-
– CO-1, CO-2, CO-5, CO-6, Pusa giant, Pusa dwarf.
– Singapore Spanish, MasmerahPuerto Rico
– glorff,Saharanpur sel, swarna roopa.
– NA-4, NA-5, NA-6, NA-7,NA-8,NA-10
– NB-1,NB-5, NB-6
crop Clonal variety
Clonal selection degeneration

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Clonal selection degeneration

  • 2. University of Agricultural Sciences, Dharwad College of Agriculture, Vijaypur Properties of asexually propagated crops, Clonal selection and Clonal degeneration Submitted to: Submitted by: Dr. M B Boranayak Brahmesh Reddy B R Dept. of genetics & Plant Breeding III yr A sec B1 batch College of Agriculture,Vijayapur College of Agriculture, Vijayapur
  • 3. Asexual reproduction Multiplication of plants without the fusion of male and female gametes is known asexual reproduction. Asexual reproduction can occur either 1. By vegetative plant parts or 2. By vegetative embryo which develop without sexual fusion.
  • 4. Rhizome : Turmeric (Curcuma domestica) Ginger(Zingiber officinale) Bulb : Garlic (Allium sativum), Onion (A. cepa)
  • 5. Corm :Bunda (Colocasia antiquorum) Arvi (Colocasia esculenta) Tuber : Potato (Solanum tuberosum)
  • 6. Significance Asexual reproduction has several advantages. 1. It leads to continuity of same genotype with great precision, because all the progeny have similar genotype and phenotype. 2. Useful in obtain large number of genetically identical individuals. 3. Promising genotype can be maintained. 4. It makes use of desirable bud mutations. Mutants can be directly released as varieties.
  • 7. Clone  Progeny of a single plant obtained by asexual reproduction is called a clone.  Crop which are propagated asexually or by vegetative means clonal crops.
  • 8. Features of Clones  Homogeneous constitution :- The progeny of clone is genetically identical and have same genetic constitution. Thus clones are homologous. No genetic variation within a clone.  Heterozygosity :-Asexually propagated crops are heterozygous so clone is also heterozygous. Progeny looks similar phenotypically but is heterozygous.
  • 9. Vigorous growth Clones have hybrid vigour which is conserved due to sexual reproduction. Most of the varieties of sugarcane and potato are hybrids Source of variation There are three sources of variation in a clone. Viz. bud mutations, mechanical mixture, and occasional sexual reproduction.
  • 10. Segregation in F1 When hybridization is done between different clones, segregation occurs in F1generation. Each F1 plant is potentially a new variety, therefore, selection is practised in F1. Wider adaptation: Clones are more adaptable to environmental conditions
  • 11. Origin of Genetic variation within Clones. 1. Somatic mutation 2. Mechanical mixture 3. Sexual reproduction 4. Segregation in F1
  • 12. Comparison among Clones, Purelines and Inbreds Particulars Clone Pureline Inbred Mode of pollination Cross pollination Self pollination Cross Pollination Natural mode of reproduction Asexual Sexual Sexual Genetic makeup of plant population Heterozygous Homozygous Hetrozygous Obtain through Asexual reproduction from a single plant Natural S.P.from a single homozygous plant Artificial self pollination and selection for several generation Maintained through Asexual reproduction Natural self pollination Artificial self pollination or Close Inbreeding Used as a variety Yes Yes No (Used for Hybrid and synthetic Hybrid development) Genetic makeup of plant with in an entity Heterozygous Homozygous Almost Homozygous
  • 13. Method of improvement • A single outstanding plant select from a population forms the basis of a new variety. • The breeding behaviour of genotype of this plant is not important since there would be no further sexual reproduction. • An improved variety that has becomes variable, or from a population produces by crossing two or more clones.
  • 14. Clonal selections Improvement of asexually propagated crops by selecting superior clones is known as clonal selection. Superior clones can be isolated from three types of materials 1. Local variety 2. Introduced variety 3. Inter crossed population
  • 15. Breeding procedure of clonal selection It involves a minimum of 7 years for complete selection of the best superior and desired clones
  • 16. SECOND YEAR:- Clones from the selected plants grown separately. a) Desirable clones selected
  • 17. THIRD YEAR :- Preliminary yield trials with standard checks. a) Selection for quality, disease resistance etc. Disease nurseries may be planted. b) Few outstanding clone selected.
  • 18. FOURTH-SIXTH YEAR :- Multilocation yield trials with standard checks. a) Best clone identified for release as a new variety.
  • 19. SEVENTH YEAR :- The best clone release as a new variety. a) Seed multiplication for distribution begins.
  • 22. Merits 1. Variety evolved by this method retains all the characters of the parental clones for several years. 2. Varieties are highly uniform like pure lines. They are highly stable because there is no risk of deterioration due to segregation and recombination 3. Effective method for genetic improvement of asexually propagated crops.
  • 23. Merits (contd..) 4.Useful in isolation the best genotype from a mixed population of asexually propagated crops. 5.The selection scheme is useful for maintaining the purity of clone.
  • 24. Demerits:- 1. selection method utilizes the variability already present in the population, and it has not been devised to generate variability. 2. Genetic makeup cannot be improved by this method without hybridization. 3. Varieties developed by clonal selection are highly prone to new of a disease.
  • 26. Clonal Degeneration The loss in vigour and productivity of clone with time is known as clonal degeneration.  Clonal degeneration= Degeneration is inherented in the clone.  Degeneration may result from 1) Mutation 2) Viral diseases 3) Bacterial diseases
  • 27. • Pidi Monthan is a clonal selection from Monthan. • High Gate is a clonal selection from Mitchel. • Kurfi Red Potato is a clonal selection from Darjeeling Red Round. It was propagated from a single disease free plant. • Kurfi Safed is a clonal selection from potato variety Phulwa. • Bombay Green Banana is a bud selection from Dwarf Cavendish. Major achievements
  • 28. Achievements in clonal selection:- Crop  Mango:- .  Grapes:-  Guava:-  Sapota:- Clonal variety – Dasehari-51, Niranjan, Cardoz mankurad, Payur-1 – Pusa seedless, Thomson seedless, Perlet, Tas –e- ganesh, Sonaka, Rao sahebi, dilkhus. – L-49 Jyoti, Alandi, S-1, Arka mridula, – Cricket ball, Kirthi bharti, Badami, Baramasi, Guthi, Thagarampudi.
  • 29.  Papaya:-  Pineapple:-  Litchi:-  Aonla:-  Bael:- – CO-1, CO-2, CO-5, CO-6, Pusa giant, Pusa dwarf. – Singapore Spanish, MasmerahPuerto Rico – glorff,Saharanpur sel, swarna roopa. – NA-4, NA-5, NA-6, NA-7,NA-8,NA-10 – NB-1,NB-5, NB-6 crop Clonal variety