This document is an introduction to the 13th edition of the World Bank publication "Doing Business 2016". It investigates regulations that enhance or constrain business activity across 189 economies. The report provides quantitative indicators on 11 areas of the business environment, with the goal of informing government policy to design sound business regulations and encourage research. It compares regulations for domestic firms, covering starting a business, dealing with construction permits, getting electricity, registering property, getting credit, protecting minority investors, paying taxes, trading across borders, enforcing contracts, and resolving insolvency.