Antimicrobial activity of some medicine plant
- 1. DR. HARISINGH GOUR VISHWAVIDYALAYA SAGAR (M P) (A Central University) DEPARTMENT OF BIOTECHNOLOGY Course Name :- Semester long dissertation/ project work/practical training/field work and technical writing Course Code :- BIT-cc-421 Presentation on :- “Study on antimicrobial activity of extract of some medicinal plants” SESSION- 2023- 24 MSC- IVth SEM Under the Supervision of Dr. Poonam Dehariya Submitted to Submitted by DR. CP Upadhyay Anil Sen Department of biotechnology Reg. No :-Y21262003
- 2. Table of Content Introduction Material and Method Result and Discussion Conclusion References
- 3. Introduction :- Medicinal plants are defined as a group of plants that possess some special properties that qualify them as asticles of drugs and therapeutic agent. PHYTOCHEMICALS o “Phyto” – plant o Naturally occurring, biologically active chemicals compounds in plants. o Phytochemicals are beneficial to human health as responsible for the disease protection through various modes of action. o Plants are rich in a wide variety of secondary metabolites such as tannins terpenoids, alkaloids and flavonoids. o These Compounds from herbs , spices and plant extracts have been shown to possess antimicrobial properties against a wide range of harmful microorganisms.
- 4. What is the antimicrobial activity ? Antimicrobial activity refers to the process of killing or inhibiting the disease causing microbes. Various antimicrobial agents are used for this purpose. Antimicrobial may be anti - bacterial, anti fungal or antiviral. They all have different modes of action by which they act to suppress the infection
- 5. material and Methods Collection of plant material Fresh leaves of selected plants Azadiracta indica, withania somnifera, ocimum sanctum. Hibiscus rosa sinensir and aloevera, free from disease were collected from botanical garden ( dept. of botany) Sagar (M.P.). The plants parts were washed two times thoroughly with distilld water
- 6. PRA parathion of plant extract by solvent extraction method • Powdered leaves were dissolved in different solvents in 1:10 ratio • (1gm powder : 10ml solution) in 5 wash bottles. • Bottles were then kept for incubation in dark for 24 hours. • These extract were then dissolved in DMSO and were then transferred in microcentrifuge tubes. • Further the extract were screened for antibacterial properties by using 100mg/ml of each sample
- 7. Antibiotic sensitivity Screening NAM(nutrient agar media) plates were prepared. Further the solvents controls and extract were screened for their antibacterial properties with slightly modification in it. where, streptomycin and water used as the positive and negative controls. The plates were left for incubation at 370 °C.
- 8. Result and observation :- The metabolite were extracted from the powder of leaves of Azadirachta indica, ocimum sanctum, Withania somnifera, Hibiscus rosa-sinensis and aloe vera plants and preserved at 37°C for the screening against bacterial pathogens E.coli and P.aeuginosa During the screening of solvents against the bacterial strains inhibitory property. Whereas a positive control (streptomycin) show inhibitory activity, mention in (table-1) similarly the extracted compounds at 24hours were as good extraction process (table 2 )
- 9. Table:- Antibacterial Screening of solvents positive controls and negative controls Sample Zone of inhibitions (mm)/cm E. coli P. aeruginosa streptomycin (+ve) 40(mm) (4cm) 42(mm) (4.2cm) water (-ve) 0 0
- 10. Inhibition zone diameter in mm/cm S.no. Plant extract name Azadirachta indica Ocimum sanctum Hibiscus rosasinensis Withania somnifera Aloe vera Test organism 1. E.coli 20mm 21mm 16mm 15mm 17mm 2. Pseudomonas 18mm 28mm 18mm 17mm 19mm Table-2 antimicrobial activity of different plant extracts against selected microbes
- 11. Fig.1 zone of inhibition of (A) Azadirachta indica (B) ocimum sanctum (C) withania somnifera (D) hibiscus rosa-sinencis (E) Aloe vera against pseudomonas
- 12. Fig.2 zone of inhibition of (A) Azadirachta indica (B) ocimum sanctum (C) withania somnifera (D) hibiscus rosa- sinencis (E) Aloe vera against E.coli
- 13. Discussion –:- Five plants were studied for antibacterial activity against two gram negative bacteria, using water solvent for extraction process. the antibacterial properties of extracted metabolites were carried out at 24 hours. During the analysis of the extracts of 24 hours, there was positive inhibition were expressed by the ocimun sanctum extract, 27.5mm,28mm against E.coli and pseudomonas. Ocimun sanctum has more zone of inhibition against E.coli and pseudomonas than other and the less zone of inhibition withania somnifera against E.coli.
- 14. Conclusion :- The study revealed about the various bioactive compounds and it's different catastrophic scenarios. this also reveals about the importance of solvent during solvent extraction of bioactive compounds.
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