Brain plotting brochure 2019
- 1. Contact : [email protected] Human data from preclinical stages To that end, we have developed in vitro and ex vivo tools from fresh Human brain tissues, such as a Blood-Brain Barrier (BBB) proprietary model, and fresh Human brain slices which preserve physiological functional properties. Our PBPK (Physiologically Based PK) model, Brain Exposure Prediction model (BEP), provides also in vivo time-curves of drug free concentration in the brain based on in vitro generated PK brain parameters. BrainPlotting is a CRO for screening purposes or for custom R&D BrainPlotting supports you in designing preclinical development plans for your drug Brain Exposure Prediction Our core business is the study of pharmacokinetics (PK) in the Human brain at early preclinical stage by using the most accurate predictive models and most recent tools. Endocytosis or Exocytosis Facilitated transportEfflux Simple diffusion Paracellular pathway Tight junctions Blood Stream Brain Parenchyma Brain Extracellular Fluid (ECF) Glial & Neuronal cells Brain Intracellular Fluid (ICF) BBB To reach its target, the molecule of interest has to cross the BBB and enter the extra-cellular (ECF) or intra-cellular fluids (ICF) of neural cells through specific transportation mechanisms fu,plasma fu,ecf fu,icf Measures of Permeabilities through the BBB Measure of permeability coefficients (Pe) IN & OUT, and efflux ratio (ER). Characterization of tight junctions and transporters expression Evaluation of functional properties as Paracellular & Transcellular pathways, ABC- and SLC- transporters BLOOD: A BRAIN: B in vitro BBB model Determination of Unbound fractions (fu) Study of the behavior of the molecule in plasma, brain homo- genate and fresh brain slices: - Measures of fu plasma & fu brain - Fu determination in brain Intra & Extra Cellular Fluids ECF surrounding brain cells Prediction of unbound concentration of your compound in the Human brain parenchyma. To that end we developed a PBPK model based on in vivo PK, Human data and in vitro specific informations Brain/Plasma Ratio defined for your product Time www.brainplo2ng.com