Cell line culture

Cell line culture

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  Cell line Culture
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  CELL CULTURE  Cellculture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment  The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation
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  Primary Cell Culture •Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate. • The cells have to be subcultured (i.e., passaged) by transferring them to a new vessel with fresh growth medium to provide more room for continued growth.
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  Cell Line • Afterthe first subculture, the primary culture becomes known as a cell line or subclone. Cell lines derived from primary cultures have a limited life span. • cells with the highest growth capacity predominate, resulting in a degree of genotypic and phenotypic uniformity in the population
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  Cell Strain • Ifa subpopulation of a cell line is positively selected from the culture by cloning or some other method, this cell line becomes a cell strain. • A cell strain often acquires additional genetic changes subsequent to the initiation of the parent line.
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  Types of Cellline • Finite Cell line • Continious cell line
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  Finite Cell line •Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite
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  Continious cell line •some cell lines become immortal through a process called transformation • When a finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes a continuous cell line
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