Chapter 2 covers assembly language programming, detailing the role of assemblers in translating symbolic source code into machine code and addressing the forward reference problem. It describes various assembler types, particularly for the x86 architecture, and outlines the features of models such as the tiny and small models used to define program structure. Additionally, the chapter discusses memory models, data definitions, and the design of instruction sets, specifically highlighting the complexities involved in instructions for the 8086 processor.