Cryopreservation
Definition
“Cryopreservation is the use of very low
temperatures to preserve the cells and tissues that
are structurally intact.”
What is Cryopreservation?
At such low temperatures, all the biological
activities of the cells stop and the cell dies.
Cryopreservation helps the cells to survive
freezing and thawing.
Cryopreservation Process
In this process, biological materials including cells,
oocytes, spermatozoa, tissues, ovarian tissues, pre-
implantation embryos, organs, etc. are kept in extremely
cold temperatures without affecting the cell’s viability.
Dry Ice and liquid nitrogen are generally used in this
method.
Cryopreservation Steps
Selection of material
Addition of cryo-protectant
Freezing –
Storage in liquid nitrogen–
Thawing- The
Benefits of Cryopreservation
Fertility treatments.
Minimal space and labour required.
Safety from genetic contamination.
Safeguards genetic integrity of
valuable stains.
Benefits of Cryopreservation
Biological samples can be preserved for a longer
period of time.
Protects the samples from disease and
microbial contamination.
Prevents genetic drift by cryopreservation of
gametes, embryos, etc.
Applications of Cryopreservation
Seed Bank.
Gene Bank.
Blood transfusion.
In vitro fertilization.
Organ transplantation.
Artificial insemination.
Storage of rare germplasm.
Freezing of cell cultures.
Conservation of endangered plant species.
Biodiversity conservation.

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