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![Services :
Patent deposits (bacteria , fungi , yeasts)
Storage service (bacteria , fungi)
Identification (bacteria [aerobic bacteria])
31
Aysha Riza & Rinu Rolly
St Mary's College Thrissur
Training :
• Management of a culture collection
• Culture and preservation methods
• Identification](https://image.slidesharecdn.com/culturecollectioncenternationalinternational2-210201085442/75/Culture-Collection-Center-National-and-International-31-2048.jpg)
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Culture Collection Center National and International
- 1. 1 Aysha Riza &Rinu Rolly St Mary's College Thrissur
- 2. • Culture collectionare centers that provide authentic examples of organism that can be grown or maintained in the laboratory ; they normally have a public service role and provide associated information and services • Prof. Frantisek Karl was the first one to collect the culture . Aysha Riza & Rinu Rolly St Mary's College Thrissur 2 https://en.wikipedia.org/wiki/Karel_Franti%C5%A1ek_Koch Karl Frantisek Koch Born : 29 June 1890 Died : 24 January 1981 Nationality : Czech
- 3. 3 Aysha Riza &Rinu Rolly St Mary's College Thrissur • Culture Collections aims at acquisition , preservation, authentication , production preservation, cataloguing and distribution of standard reference microorganism , cell lines and other materials for research and repositories of type strains.
- 4. • According toWorld directory of culture collections (Sixth Version 2014): * Culture collection : 802 * Countries and region : 78 *Microorganism :3272863 * Bacteria :1429816 * Fungi : 872094 * Virus :39491 * Cell line : 33020 Aysha Riza & Rinu Rolly St Mary's College Thrissur 4
- 5. • Collection andmaintenance of important and useful organism • Maintain culture in viable and contaminant free state. • To make available at nominal cost to researcher ,workers, teachers ,students or industries . • Preparation of their information catalogue . • Comparison of microorganisms 5 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 6. • Initiative ofgovernment department of biotechnology. • Housed at National Center of Cell Science (NCCS), Pune • Depository of microorganism (for isolates generated from microbial prospecting and screening program initiated by DBT) Aysha Riza & Rinu Rolly St Mary's College Thrissur 6 www.nccs.res.in Microbial collection Center, Pune
- 7. • Status ofcollection – governmental • Sponsor – Department of Biotechnology ,Ministry of Science and Technology, Government of India Aysha Riza & Rinu Rolly St Mary's College Thrissur 7 Services : Storage (bacteria, fungi) Distribution Identification
- 8. Trainings : Cultureand preservation methods Identification 8 Aysha Riza & Rinu Rolly St Mary's College Thrissur NUMBER OF STRAINS IN MCC Type Holding strains (Total number) Bacteria 149314 Fungi 15338
- 9. • Established in1986 . • MTCC roots at Institute of Microbial Technology (IMTECH), Chandigarh . • Sponsor – Department of biotechnology and Government of India and Council of Scientific and Industrial Research . • Status – Semi governmental Aysha Riza & Rinu Rolly St Mary's College Thrissur 9 https://www.imtech.res.in/focus-area/microbial-type-culture- collection Microbial Type Culture collection and Gene bank , Chandigarh
- 10. Main objectives Act asdepository To supply authentic microbial cultures To provide related services to the scientist working in research institutions universities and industries . 10 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 11. Services : Distribution(bacteria ,fungi ,plasmids) Identification Patent deposits Storage services Training : Culture and preservation methods Genetic engineering Identification bacterial genetics 11 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 12. NUMBER OF STRAINSIN MTCC Type Holding Strains (Total number) Algae 1124 Bacteria 1245 Fungi 575 Yeasts 85 12 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 13. • Established in1951 . • One of the oldest biological resource center . • Status – Governmental • Housed at National Chemical Laboratory (CSIR) New Delhi Services: Aysha Riza & Rinu Rolly St Mary's College Thrissur 13 Patent deposits (Bacteria ,yeast , fungi) Storage services (microbial strain) Distribution (bacteria ,yeast ,fungi ,algae)
- 14. Trainings : Managementof culture collection Culture and preservation method Genetic engineering Systematics. Identification 14 Aysha Riza & Rinu Rolly St Mary's College Thrissur NUMBER OF STRAINS IN NCIM Type Holding Strains ( Total number) Algae 20 Bacteria 1400 Fungi 950 Yeasts 600
- 15. • Established in1962. • Status of the collection – ICAR. • Housed at National Dairy Research Institute ,Karnal Haryana . • Sponsor – N.D.R.I, Karnal and ICAR , New Delhi. Aysha Riza & Rinu Rolly St Mary's College Thrissur 15 https://www.showincity.com/venues/v-18667/national-dairy-research- institute National Culture of Dairy Collection , Harayana
- 16. Services : Distribution(bacteria, fungi, yeast, dairy starters ). Training : Culture and preservation method Quality control Use of computers in culture collections Preservation , propagation and quality control of Dairy starter cultures Preparation of fermented milks 16 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 17. NUMBER OF STRAINSIN NCDC Type Holding strains (Total number) Bacteria 400 Fungi 15 Yeasts 20 Dairy starter cultures 25 17 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 18. • Established in2008 • Initiative by Department of Science and Technology at Agharkar Research Institute, Maharashtra. • NFCCI aims at conserving fungi as resource pool for future research and related projects. • Housed at MACS’ Agharkar Research Institute , Maharashtra • Status – Governmental ,National Facility • Sponsor – Department of Science and Technology ,Government of India and MACS’ Agharkar Research Institute , Pune , India . 18 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 19. Services : Storageservices (micro and macro fungi) Distribution Identification NUMBER OF STRAINS IN NFCCI Type Holding strains (Total number) Fungi 3050 19 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 20. Training: Management ofa culture collection Culture and preservation methods Quality control Use of computers in culture collection Systematics Identification Molecular systematics Fermentation and isolation of secondary products 20 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 21. • Established in1925. • Largest general culture collection in the world. • Serves as centre for acquisition , preservation, authentication and distribution of microbial specimens standard reference microorganism , cell lines and other materials for research and development . Aysha Riza & Rinu Rolly St Mary's College Thrissur 21 American Type Culture Collection ,USA https://www.atcc.org/~/media/PDFs/webinars/Presentations/2017/M ycoplasma%20Detection%20Webinar.ashx
- 22. • Status –Private non profit organization. • Designated as first International Depository Authority (IDA) (International Budapest Treaty for biotechnology patent ,1981) Programs: 22 Aysha Riza & Rinu Rolly St Mary's College Thrissur Collection , Research and service program . Professional Service program . Information Service program
- 23. Services: Patent deposits . Storageservices (safe deposit). Identification (authentication services). 23 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 24. Training: Management ofculture collection Culture and preservation methods Quality control and Genetic engineering Karyotyping. Use of computer in culture collections . Cytogenetics. Diagnostic virology Fermentation Hybridomas and monoclonal antibodies Medicinal mycology 24 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 25. NUMBER OF STRAINSIN ATCC Type Holding strains (Total number) Bacteriology: Bacteria & bacteriophage 18000 Cell biology: Cell lines & hybridomas 4300 Molecular biology: Recombinant DNA Material 0 Mycology &botany : Filamentous fungi & yeasts 46000 Plant tissue 79 Seeds 1000 Protists (including algae) 2000 Virology: Animal virus, chlamydiae , rickettsiae & antisera 2200 Plant viruses & antisera 1500 25 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 26. • Established in1981 • Housed and maintained by RIKEN Bio Resource and Research Center at Tsukuba ,Ibaraki ,Japan • Status – semi-governmental • Sponsor – The Japanese government (Ministry of education , culture, sports , science and technology ) Aysha Riza & Rinu Rolly St Mary's College Thrissur 26
- 27. • This divisioncollects , preserves and distributes cultured microbial strain as one of the leading culture center in the world • Strains held at JCM are limited to those classified as Risk 1 and Risk 2 • ISO Certified for its quality management system 27 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 28. Services : Distribution Training: Culture and preservation methods Systematics. Identification 28 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 29. NUMBER OF STRAINSIN JCM Type Holding strains (Total number) Bacteria 18822 Yeasts 4208 Archaea 838 Microalgae 26 Filamentous fungi 3904 29 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 30. • Established in1963. • Rooted and maintained by Masaryk University • Status of collection – university Catalogue of cultures include : Bacteria and Archea. Filamentous fungi and yeast. Bacteriophages. Fungi ( deuteromycetes, ascomycetes, mucorales) 30 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 31. Services : Patentdeposits (bacteria , fungi , yeasts) Storage service (bacteria , fungi) Identification (bacteria [aerobic bacteria]) 31 Aysha Riza & Rinu Rolly St Mary's College Thrissur Training : • Management of a culture collection • Culture and preservation methods • Identification
- 32. NUMBER OF STRAINSIN CCM Type Holding strains(Total number) Bacteria 2600 Fungi 750 Yeasts 22 32 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 33. • Established in1920 • Serves to “ provide a trustworthy source of authentic bacteria for use in scientific studies”. • NCTC is one of the four culture collections of public health England • Status – governmental • Sponsor – department of health 33 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 34. • It islongest established collection of its type anywhere in the world • It also serves as United Nations Educational , Scientific and Cultural Organization(UNESCO) Microbial Resource Center (MIRCEN) • NCTC holds nearly 6000strains most of which have caused infections in humans or animals and most of them are medical , scientific and veterinary importance world wide • It also serves as an International Depository Authority (IDA) patent depository for bacterial cultures. 34 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 35. Strains grouped byspecific characteristics Antibacterial susceptibility control and resistance reference strains Control strains for the microbiological testing of water samples for Pseudomonas aeruginosa. Clostridium difficile strains – Ribotype and toxin status NCTC reference strains for standards for microbiology investigations (UK- SMI) World Data Center for Microorganism (WDCM) reference strains 35 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 36. Services Patent deposits(bacteria) Storage service (bacteria) Distribution (bacteria) Identification (bacteria) NUMBER OF STRAINS IN NCTC Type Holding strains (Total number) Bacteria 5000 Plasmids 600 36 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 37. • Established in1979 as central culture collection network of various collection. • Rooted at institution of microbiology , China academy of science • Status – governmental • Sponsor – Chinese academy of sciences Aysha Riza & Rinu Rolly St Mary's College Thrissur 37 http://www.cgmcc.net/ China General Microbial Culture Collection center ,China
- 38. • ISO certified •Focus – to preserve , supply and maintain living microbial resources . Contribute to scientific community by providing high quality microbial source to research fields. • Maintain approximately 40000 by the end of 2013 including bacteria,archea, fungi • Strains held at CGMCC classified to risk 1 0r 2 group • Information of strain available at online catalogue database of CGMCC 38 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 39. • 8700 strainfor patent purpose • To improve and expand collection continously CGMCC exploit new resources from various environment , describing microbial taxa, accept useful microbes researcher and applied sectors • All strains are subjected to extensive quality control 39 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 40. Services : • Patentdeposit (Archea ,Bacteria, Yeasts, Fungi, Algae, Viruses, plasmids, cell lines, Plant seed) • Storage services (Archea ,Bacteria, Yeasts, Fungi) • Distribution (Archea ,Bacteria, Yeasts, Fungi , cell lines) • Identification (Archea ,Bacteria, Yeasts, Fungi ) NUMBER OF STRAINS IN CGMCC Type Holding strains Bacteria 19820 Fungi 16750 Yeast 5360 Cultures for patent purpose 11976 40 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 41. • Avinash Sharmaand Yogesh Shouche. (2014). Microbial Culture Collection (MCC) and International Depositary Authority (IDA) at National Centre for Cell Science , Pune . Indian Journal of Microbiology .129-133. • https://mtccindia.res.in/ • https://www.ncl-india.org/files/ncim/Default.aspx • http://www.ndri.res.in/ncdc/Members/NCDC_Catalogue_2016.pdf • http://nfcci.aripune.org/ • http://nfcci.aripune.org/about_us.php • http://gcm.wfcc.info/ • http://www.wfcc.info/index.php/wdcmdb/ • http://www.wfcc.info/ccinfo/collection/by_country/u/ • http://www.wfcc.info/ccinfo/collection/by_country/j/ • http://www.wfcc.info/ccinfo/collection/by_country/c/ • http://www.wfcc.info/ccinfo/collection/by_country/i/ • http://www.nccs.res.in/mcc/index.html • http://www.imtech.ernet.in/mtcc/ • http://www.ndri.res.in/ • http://http.atcc.org/ • http://jcm.brc.riken.jp/ • http://www.szu.cz/cnctc/ • www.phe-culturecollections.org.uk • http://www.cgmcc.net 41 Aysha Riza & Rinu Rolly St Mary's College Thrissur
- 42. 42 Aysha Riza &Rinu Rolly St Mary's College Thrissur