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Dihybrid Cross
Crossing two different traits
Hair length and eye color
Hair Length*
• HH – Hh short hair (dominant trait)
• hh long hair (recessive trait)
* Yes I realize that this is not an
actual heritable trait. Just go with it.
Eye Color
• RR – Rr black eyes
(dominant)
• rr red eyes
(recessive)
The Parents
• Both parents are heterozygous for both traits.
• Hh Rr
H h R r
FOIL the parents
H R rhH R rh
Use the concept you learned in math class.
H h R r
FOIL the parents
H
R rh
HR r
h
H h R r
FOIL the parents
H R rhHR r h
These are the different combinations of the two
traits that the parents’ sex cells will contain.
Since both the parents had the same genotype,
we only needed to foil one parent. This rarely
happens.
Now we place each parent’s alleles on the
Punnett square. I usually place the father on the
top and the mother on the side. They will be
color coded so that you can tell the difference.
HR
HR
Hr hR hr
Hr
hR
hr
HR
HR
Hr hR hr
Hr
hR
hr
We copy one parent’s alleles into the boxes.
HR
HR
Hr hR hr
Hr
hR
hr
H R
H R
H R
H R
H r
H r
H r
H r
h R
h R
h R
h R
h r
h r
h r
h r
(These are the alleles from the parent on top)
HR
HR
Hr hR hr
Hr
hR
hr
H R
H R
H R
H R
H r
H r
H r
H r
h R
h R
h R
h R
h r
h r
h r
h r
We copy the other parent’s alleles into the boxes.
HR
HR
Hr hR hr
Hr
hR
hr
HHRR
HHRr
HhRR
HhRr
HHRr
HHrr
HhRr
Hhrr
HhRR
HhRr
hhRR
hhRr
HhRr
Hhrr
hhRr
hhrr
Here are both parents’ alleles. Notice it does not matter which parent is first; only
that the dom. allele is in front and that each trait is together.
What are the kids going to look like?
• HHRR
• HHRr
• HhRR
• HhRr
• HHrr
• Hhrr
• hhRR
• hhRr
• hhrr
Short hair, black eyes
Short hair, red eyes
Long hair, black eyes
Long hair, red eyes
Genotypes
Phenotypes
HR
HR
Hr hR hr
Hr
hR
hr
HHRR
HHRr
HhRR
HhRr
HHRr
HHrr
HhRr
Hhrr
HhRR
HhRr
hhRR
hhRr
HhRr
Hhrr
hhRr
hhrr
Do you see the pattern?
What are the kids going to look like?
• HHRR
• HHRr
• HhRR
• HhRr
• HHrr
• Hhrr
• hhRR
• hhRr
• hhrr
Short hair, black eyes
9
Short hair, red eyes
3
Long hair, black eyes
3
Long hair, red eyes
1
Genotypes
Phenotypes

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Dihybrid cross Tutorial