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Dinitro salicylic acid (DNSA) method
The document describes a Dinitrosalicylicacid (DNSA) method for testing sugar concentration using a colorimetric assay. Various concentrations of a working sugar standard from 400-2000 μg are prepared and reacted with DNSA reagent and boiled, then the optical density of each sample is measured at 540 nm to generate a standard curve relating sugar concentration to absorbance.
Dinitro salicylic acid (DNSA) method
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- 5. Particulars B S1S2 S3 S4 S5 Test Working standard (in ml) - 0.2 0.4 0.6 0.8 1.0 1.0 Concentration in std (in μg) - 400 800 1200 1600 2000 - Water (in ml) 2 1.8 1.6 1.4 1.2 1.0 1.0 DNSA reagent (ml) 1 1 1 1 1 1 1 Boiling water bath At 90˚ C for 1.5 min. Water (ml) 17 17 17 17 17 17 17 OD read at 540 nm