Dna fingerprinting
Download as PPTX, PDF25 likes978 views
DNA fingerprinting is a technique used to identify individuals based on their unique DNA patterns. It involves isolating DNA from a sample, cutting the DNA into fragments of varying lengths using restriction enzymes, sorting the fragments by size through gel electrophoresis, transferring the fragments to a nylon membrane, and probing the membrane to produce a pattern - the DNA fingerprint - that can be used to identify an individual. DNA fingerprinting has uses in personal identification such as identifying casualties or missing military personnel.
More Related Content
Similar to Dna fingerprinting (20)
Dna fingerprinting
- 1. Dr. Prafulla Katkar M.Sc. Ph.D(Italy), Post Doc Assistant Professor Department of Microbiology Guru Nanak College of Science, Ballarpur MS- 442701
- 2. What is DNA Fingerprinting? DNA Fingerprinting is a way to identify a certain individual, rather than simply identifying a species or a particular trait.
- 3. 6 Steps to DNA Fingerprinting Step 1: Isolation of DNA DNA must be recovered from cells or tissue. Only a small amount of blood, hair, or skin is needed.
- 4. 6 Steps to DNA Fingerprinting Step 2: Cutting, Sizing, and Sorting Restriction enzymes are used to cut the DNA at specific places. In a process called electrophoresis, DNA pieces are sorted out by their size. Then the DNA pieces are passed through a gel made of seaweed agarose.
- 5. 6 Steps to DNA Fingerprinting Step 3: Transfer of DNA to Nylon The DNA pieces are transferred to a nylon sheet by placing the sheet on the gel and soaking them overnight.
- 6. 6 Steps to DNA Fingerprinting Step 4-5: Probing Adding radioactive or colored probes to the nylon sheet produces a pattern, which is the DNA Fingerprint. Each probe only sticks to one or two specific places on the sheet.
- 7. 6 Steps to DNA Fingerprinting Step 6: DNA Fingerprint The final DNA fingerprint is built by using several probes. It resembles bar codes.
- 9. Uses of DNA Fingerprinting Personal Identification The Indian armed services are just beginning a program where they collect DNA fingerprints from all personnel for later use, incase they need to identify casualties or missing people.