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Environmental Genomics
- 1. Environmental Genomics™ Using Advanced DNA Sequencing to Conduct a Microbial Census Aster Bio www.asterbio.com [email protected] 713.734.0082
- 2. Environmental Genomics™ Testing – A True Microbial Census • DNA-based test using genetic material from the 16S rDNA region in bacteria • Identifies what microbes are growing in the sample • Gives a relative frequency of each microbial form • Includes cultures that are difficult or impossible to grow on conventional plates © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 1
- 3. How Does Environmental Genomics Relate to Other Monitoring Methods? © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 2 1st Generation • MLSS • SV30 • Nutrient residuals 2st Generation • ATP • Respirometer (OUR) • Microscopic Exam 3rd Generation • 16S Metagenomics • FISH (DNA Markers) Better Direct Information on Biomass
- 4. Benefits from Environmental Genomics™ Testing © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 3 • Identification of microbes associated with bulking or high EPS production • Evaluate how any system change impacts the biomass • Changes in MCRT • Temperature • D.O. & mixing • Investigate Biological Nutrient Removal (BNR) efficiency • Nutrient formulation changes • New waste streams • Impact of bioadditives • Formulation of custom bioaugmentation products
- 5. Example Applications of Environmental Genomics™ Testing © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 4
- 6. Identify Sources of Filamentous Bulking © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 5 An industrial facility operating an extended aeration activated sludge unit (ASU) was experiencing sustained filamentous bulking issues. Even with sufficient D.O. according to D.O. meter checks, filaments were causing bulking and excess secondary polymer use. Previously, the facility had used outside microscopic exam to evaluate the sludge and filaments present. Aster Bio wanted to test the Environmental Genomics platform to see if 16S could identify the filaments and other microbial forms causing bulking. Testing found the system had significant concentrations of Hyphomicrobium sp. The subsequent investigation found the bulking was response to high levels of methanol in the influent, not a previously suspected nutrient or dissolved oxygen sufficiency.
- 7. Bioremediation – Creating a Custom Consortium © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 6 In China, a former industrial chemical site had discharged wastewater in a lagoon system without any advanced biological treatment. The sludge buildup contained many recalcitrant compounds including pesticides, herbicides, PAHs, and chlorinated compounds. Initial attempts at bioremediation including adding bioaugmentation cultures had not succeeded in removing the criteria pollutants. Aster Bio worked with local remediation specialists to characterize the waste and used Environmental Genomics™ technology to identify cultures capable of treating the sludge. Using data from the testing, Aster Bio was able to create a microbial consortium that would initiate the bioremediation process and speed up site closure.
- 8. About Aster Bio We put Mother Nature to work Our bugs are better Microorganisms are Nature’s recyclers. At Aster Bio, we put Earth-friendly microorganisms to work to treat biological waste and reduce pollution and toxic compounds in wastewater, groundwater and soil. Aster Bio is the only biological solutions company to offer leading-edge multigenomic screening technologies combined with time-tested environmental microbiology techniques to create customized biochemical products for better results. The Aster Bio Difference We save our customers’ money Aster Bio scientists are experts in microbial ecology, biochemistry and waste treatment. We’ve developed a leading-edge biomass management approach, including our Environmental Genomics testing, to make biological treatments work better. We use custom bioaugmentation* formulations, micronutrients and process monitoring programs to help our customers meet their waste treatment goals. Aster Bio can maximize a customer’s treatment system’s performance while reducing chemical, utility and capital costs. We analyze the biological treatment system with a biomass evaluation/system audit and our exclusive Environmental Genomics DNA testing to develop a customized, effective bioaugmentation solution. Micro solutions for big industry challenges A spirit of continuous innovation We formulate customized solutions to optimize wastewater biomass in municipal, pulp and paper, petrochemical, food processing and organic chemical wastewater treatment systems. Our formulations are used to enhance bioremediation of soils and waters contaminated by hydrocarbons and other recalcitrant chemicals. While we make great products and have strong application technologies, Aster Bio strives to find new, better biochemical processes, organisms, and application technologies that improve on existing products and extend natural biological technologies into applications currently served only by chemical treatments. © 2003 – 2016, Aster Bio, Inc. All Rights Reserved 7 * bioaugmentation: adding cultured microorganisms into water or soil for the purpose of degrading contaminants