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Final Project Poster
- 1. Determination of Calcium in Orange Juice via EDTA Titration Chris Myers, Chm 353 Quantitative Analysis Final Project Introduction • The purpose of this experiment was to determine calcium concentrations between orange juices taken from Augsburg’s cafeteria and St. Thomas’s cafeteria. • Another purpose of this experiment was to examine and compare methods of calcium determination as both EDTA titration and AAS were used. • Calcium is the most abundant mineral in the body and is extremely important in bone and tooth strength. • The Institute of Medicine of the National Academy of Sciences found that many Americans are not meeting there daily requirements of calcium (1000 mg daily). • Foods rich in calcium are milk, yogurt, cheese, cabbage, kale, spinach, broccoli, calcium fortified juices, breads, and cereals. • So who cares more about our health; Augsburg or St. Thomas? Lets see. Results & Discussion • Since the EDTA and mole ratios were 1:1 calcium concentration was then calculated off of data collected from EDTA titration and standardization • What was found was that Augsburg orange juice had 76.49 mg calcium in 8 fluid oz and St. Thomas had 72.32 mg calcium in 8 fluid oz. • When comparing this data to the AAS data collected by Dave Bergstrand, a correlation was found as AAS data also showed Augsburg OJ having a higher calcium content (82.42mg vs 79.95mg). Methods • This experiment used EDTA titration to calculate calcium concentration. • Each orange juice solution was prepared by filtration and then a 1:1 dilution with 0.1M HCl. • An ammonia buffer (pH 10) was used along with a calmagite indicator to calculate endpoint during titration (wine red to blue). • Lastly the EDTA solution was standardized by a CaCO3 0.1 M HCl solution. References Office of Dietary Supplements. Dietary Supplement Fact Sheet: Calcium. http://ods.od.nih.gov/factsheets/Calcium-HealthProfessional/ (accessed Apr 23, 2013) Conclusion • What was found from both EDTA titration and AAS was that Augsburg had orange juice with a higher calcium concentration than St. Thomas did. • It was also found that both methods worked and had similar results. • The accuracy of the methods couldn’t be calculated due to literature values being absent from dispenser. Acknowledgement (optional) • Dave Bergstrand who performed AAS Augsburg Orange Juice St. Thomas Orange Juice Trial 1 Vol 3.7 ml Trial 1 Vol 3.4 ml Trial 2 Vol 3.6 ml Trial 2 Vol 3.5 ml Trial 3 Vol 3.7 ml Trial 3 Vol 3.4 ml Average Vol 3.67 ml Average Vol 3.47 ml Trial 1 mol 0.000041 Trial 1 mol 0.000037 Trial 2 mol 0.000040 Trial 2 mol 0.000039 Trial 3 mol 0.000041 Trial 3 mol 0.000039 Average mol 0.000040 Average mol 0.000038 Calcium (mg) 1.62 per 5 ml Calcium (mg) 1.53 per 5 ml 8 Fluid oz = 76.49 mg calcium 8 Fluid oz = 72.32 mg calcium