Albert G.J. Tacon PhD
Aquatic Farms Ltd
Kaneohe, HI 96744 USA
http//www.aquahana.com
AQUAHANA
Fish Matters: Role of Biotechnology
in Improving Nutrition
The Role of Agricultural Biotechnologies in Sustainable
Food Production Systems & Nutrition
15-17 February 2016, FAO Headquarters, Rome, Italy
AQUACULTURE
the farming of aquatic plants & animals
has been the world’s fastest growing food sector for 25 years
& is considered as an important weapon in the global fight
against malnutrition as a provider of much needed
high quality protein & other essential nutrients
Important Contribution of Aquaculture to Food Security
Aquaculture supplied 45.9 million tonnes of farmed aquatic meat in 2013 (APR 7.40%),
compared with 71.8 million tonnes from wild capture fisheries (APR 0.25%), and 310.4
million tonnes of terrestrial meat production (APR 2.46%). In China aquaculture
supplied 26.1 million tonnes of farmed aquatic meat (APR 7.33%), compared with 85.2
million tonnes of terrestrial meat production (APR 4.06%; FAO, 2016)
WORLD
356.3 mmt
CHINA
111.3 mmt
GLOBAL FARMED MEAT PRODUCTION – WORLD & CHINA
(Values given in million metric tonnes; FAO, 2016)
PIG
113.0
PIG
53.7
CHICKEN
96.1
CHICKEN
13.4
CATTLE
64.0 CATTLE
6.4
AQUATIC
45.9
AQUATIC
26.1
Definitions: Agricultural Biotechnology
•According to FAO, Agricultural Biotechnology includes:
“Any technological application that uses biological systems, living
organisms, or derivatives thereof, to make or modify products or
processes for specific use”
•For the purposes of this presentation Agricultural Biotechnology
will be used to include those activities related to Improving
Nutrition, including the improved nutrition of the feeds used for
the production of farmed aquatic species, the reduction of
potential environmental impacts and improved sustainability of
aquaculture feeds, to the fortification of the nutritional content of
aquaculture produce for direct human consumption
AQUACULTURE 2013 AGRICULTURE
>309 SPECIES>309 SPECIES
202202
1515
3535
5757
>158 SPECIES>158 SPECIES
1818
Cereals
Fruits & nuts
Roots &
tubers
Oilseeds &
pulses
Vegetables
Livestock
Finfish
Molluscs
Crustaceans
Aquatic plants
4848
1010
2626
Fed species Fed species
237 18
Fish Matters: Role of Biotechnology
in Improving Nutrition
Role of Biotechnology in Improving
Nutrition & Sustainability
of Fish Production
Through
Use of Feed Additives
Use of Novel Feed Ingredients
Reduced Imports & Environmental Impacts
Use of Fermentation Technology
for the Production of Essential
Nutrients & Feed Additives
Vitamins, pigments, antioxidants & emulsifiers
Minerals, trace elements, salt, limestone, zeolite, chelates
Amino acids, nucleotides, feeding attractants, sterols
Enzymes, gut modifiers, prebiotics, probiotics, organic acids
Immune enhancers, anti-fungal, anti-viral, anti-parasitical
Essential oils, binders, growth promoters, hormones, antibiotics
Improving the utilization of feed ingredients
Use of renewable nutrient sources
Improving the utilization of feed ingredients
Use of renewable nutrient sources
Mannanase
α - galactosidase
EXOGENOUS MICROBIAL ENZYMES
•Improved nutrient digestibility
•Improving feed efficiency
•Release of trapped nutrients
•Breakdown of anti-nutritional factors
•Improved gut health
•Reducing environmental impacts (N, P)
β – glucanase
Cellulase
Amylase
Protease
Phytase
Xylanase
Lipase
Micotoxinase
Indonesia
Use of microbial phytases to improve phosphorus digestibility and
reduce phosphorus loss to the aquatic environment
Using amino acids to reduce fishmeal use
Use of renewable nutrient sources
AMINO ACIDS
PRODUCED BY
FERMENTATION
•Arginine
•Glutamine/MSG
•Histidine
•Isoleucine
•Leucine
•Lysine
AMINO ACIDS
PRODUCED
BY
FERMENTATI
ON
•Phenylalanine
•Proline
•Serine
•Threonine
•Tryptophan
•Valine
Source: Evonik
Traditional salmon diet Modern salmon diet*
Move from traditional fishmeal and fish oil based
diets to plant-based diets for salmonids
Beneficial use of using supplemental limiting amino acids
Aquatic protein meals & oils 1-25%
Fishmeals & oil: wild & farmed
Squid meal, krill meal
Seaweed meals & products
Cultured microbial biomass
Terrestrial animal proteins &
oils
1-25%
Poultry by-products
Porcine by-products
Ruminant by-products
Terrestrial invertebrates
Terrestrial plant proteins & oils 1-25%
Oilseed protein by-products
Cereal protein by-products
Pulse protein by-products
Other plant proteins
Other plant meals & fillers 1-25%
Cereal meals & by-products
Root meals & extracts
Fruit meals & by-products
Forage & leaf meals
Feed additives 0-5%
Vitamins, antioxidants, pigments & emulsifiers
Minerals, trace elements, salt, zeolites
Amino acids, nucleotides, feeding attractants, sterols
Gut modifier, prebiotics, probiotics, acidifiers, essential
oils
Immune enhancers, anti-fungal, anti-viral, anti-parasitical
Binders, growth promoters, hormones, antibiotics
Major ingredient groups commonly used
in
AQUACULTURE FEEDS
Major ingredient groups commonly used
in
AQUACULTURE FEEDS
Source: Evonik
Source: Nutrinsic
Alltech, Brasil
DV Aqua is a complex
combination of
fermentation
metabolites and
residual yeast cells
Mannan
Oligosaccharides
Highly branched β-
glucans
Nucleotides
Fermentation
metabolites
'
' ''''''
'
'
'
Plasma''membrane'
(From'Netea'et'al''2008)'
β
Aurantiochytrium
– Contains more than 10 % DHA on a dry weight basis and
might be a good source for fish oil replacement in
formulated diets.
Heliae © 2015 – Confidential 20
Alltech Algae, Winchester, Kentucky
Heterotrophic algae - Schizochytrium
70% Fat containing 28% DHA
Source: Alltech
Objective: More sustainable fish feeds
Traditional salmon diet Modern salmon diet*
Use of dried biomass of
micro algae as fish oil replacer
Source: Evonik
PROBIOTICS
700 ha shrimp farm
Water, Pond Bottom, Feed..
Value added – processing on site
A probiotic is defined as:
- a live microbial adjunct
- which has a beneficial effect on the host
- by modifying the host-associated or ambient microbial
community
- by ensuring improved use of the feed
- or enhancing its nutritional value
- by enhancing the host response towards disease
- or by improving the quality of its ambient environment
Verschuere et al. 2000. Microbiology and Molecular biology Reviews 64:655–671
Definition of aquaculture probiotics
Ojai - Mexico
Fermentation of Feed Ingredients to
increase nutritional value
Fermentated Soybean Meal
Use of Solid State Fermentation to better utilize locally
available agricultural feed & food wastes, & for the
reduction of the anti-nutritional factors present in plant
proteins such as soybean, rapeseed, lupin & pea
Fish Matters: Role of Biotechnology
in Improving Nutrition
Role of Biotechnology in Improving
Conservation & Nutrient Content of
Fish for Human Consumption
Through
Fermentation – Fish Sauce
Use of Algal-DHA & Chelated-Minerals
Garum: Fermented Fish Sauce for the Ancient Roman Masses
Fish fermentation allowed the ancient Romans to store their fish surplus for long
periods. Making garum was simple; placing fish (mackerel, sardines, anchovies, or
entrails) in a barrel with salt at a 5:1 ratio, placing a weight on top of the mixture,
and then allowing to ferment for 2-3 months.
By this time the fish will ferment and liquify, creating an umami flavor similar to
that of parmesan, and a slightly pungent smell. The liquid can then be removed,
and the remaining residue used to make a second batch of fish sauce.
Wood vats line the Khai Hoan fish sauce factory, where the fish are fermented
Source: Alltech
Microalgae: Haematococcus pluvialis
Astaxanthin – natural antioxidant
Astaxanthin is 3 % DW
Microbial chelated
minerals:
Selenium
Zinc
Chromium III
Iodine
NUTRITION is the cornerstone that effects the
Health & Wellbeing of all People: both Rich & Poor
JAPAN USA
Calories (kcal/day) 2,719 3,639 +++
Animal protein (g/day) 49.1 70.7 +++
Animal fats (g/day) 33.9 68.3 +++
Terrestrial meat (kg/year) 48.8 117.6 +++
Fish & seafood (kg/year) 53.7 +++ 21.6
Aquatic animal fat (g/day) 6.14 +++ 1.42
Aquatic plants (kg/year) 0.99 +++ 0
Per capita food supply in Japan & USA
(FAO Food Balance Sheets, 2016)
it is not by chance that Japan, the country with the highest reported life expectancy and
with one of the world’s lowest incidences of obesity & deaths from heart related
illnesses, is also one of the worlds top consumer of aquatic animal products & farmed
aquatic plants: comparison made with the USA
• AQUATIC FOOD PRODUCTS represent one of the world’s
most nutritious & healthy foods
• According to FAO/WHO Joint Expert Consultation on the
Risks & Benefits of Fish Consumption, there is convincing
evidence that
 Fish consumption reduces the risk of death from coronary
heart disease & consumption by women reduces the risk of
suboptimal neurodevelopment in their offspring;
 There is also emerging, possible or probable evidence, that
fish consumption may also reduce the risk of ischaemic
stroke, non-fatal coronary heart disease events, congestive
heart failure, atrial fibrillation, cognitive decline,
depression, anxiety & inflammatory diseases
NUTRITIONAL COMPOSITION OF AQUATIC FOODS
Compared with terrestrial farmed meat products, aquatic animal &
plant foods (whether captured or cultured) are:
•Rich source of high quality animal protein with a mean of 17.3%
compared with 13.8% for terrestrial meat;
•Rich source of long chain omega-3 fatty acids - DHA;
•Rich source of essential minerals, including Calcium, Phosphorus,
Sodium, Magnesium, Iron, Zinc, Manganese, Copper, Iodine,
Chromium, Fluorine;
•Rich source of essential vitamins, including Vitamin A, Vitamin
D, Vitamin B12, Folic acid, Vitamin E, Choline
Aquatic plants
Cephlapods frozen
Molluscs frozen
Crustaceans frozen
Marine fish nes fillet
Pelagic fish fillet
Demersal fish fillet
Freshwater/diadromous fish fillet
Cows milk
Hens egg
Poultry meat
Turkey meat
Pig meat
Muttton & lamb
Duck meat
Chicken meat
Beef boneless
g/100g
Composition of different purchased foods
(FAO, 2001 )
Mainly polyunsaturated
omega-3 fatty acids
Mainly saturated
omega-6 fatty acids
Thank you
http://www.aquahana.com
Fish Matters: Role of Biotechnology in
Improving Nutrition

Fish for all: role of biotechnology in improving nutrition

  • 1.
    Albert G.J. TaconPhD Aquatic Farms Ltd Kaneohe, HI 96744 USA http//www.aquahana.com AQUAHANA Fish Matters: Role of Biotechnology in Improving Nutrition The Role of Agricultural Biotechnologies in Sustainable Food Production Systems & Nutrition 15-17 February 2016, FAO Headquarters, Rome, Italy
  • 2.
    AQUACULTURE the farming ofaquatic plants & animals has been the world’s fastest growing food sector for 25 years & is considered as an important weapon in the global fight against malnutrition as a provider of much needed high quality protein & other essential nutrients Important Contribution of Aquaculture to Food Security
  • 3.
    Aquaculture supplied 45.9million tonnes of farmed aquatic meat in 2013 (APR 7.40%), compared with 71.8 million tonnes from wild capture fisheries (APR 0.25%), and 310.4 million tonnes of terrestrial meat production (APR 2.46%). In China aquaculture supplied 26.1 million tonnes of farmed aquatic meat (APR 7.33%), compared with 85.2 million tonnes of terrestrial meat production (APR 4.06%; FAO, 2016) WORLD 356.3 mmt CHINA 111.3 mmt GLOBAL FARMED MEAT PRODUCTION – WORLD & CHINA (Values given in million metric tonnes; FAO, 2016) PIG 113.0 PIG 53.7 CHICKEN 96.1 CHICKEN 13.4 CATTLE 64.0 CATTLE 6.4 AQUATIC 45.9 AQUATIC 26.1
  • 4.
    Definitions: Agricultural Biotechnology •Accordingto FAO, Agricultural Biotechnology includes: “Any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use” •For the purposes of this presentation Agricultural Biotechnology will be used to include those activities related to Improving Nutrition, including the improved nutrition of the feeds used for the production of farmed aquatic species, the reduction of potential environmental impacts and improved sustainability of aquaculture feeds, to the fortification of the nutritional content of aquaculture produce for direct human consumption
  • 5.
    AQUACULTURE 2013 AGRICULTURE >309SPECIES>309 SPECIES 202202 1515 3535 5757 >158 SPECIES>158 SPECIES 1818 Cereals Fruits & nuts Roots & tubers Oilseeds & pulses Vegetables Livestock Finfish Molluscs Crustaceans Aquatic plants 4848 1010 2626 Fed species Fed species 237 18
  • 7.
    Fish Matters: Roleof Biotechnology in Improving Nutrition Role of Biotechnology in Improving Nutrition & Sustainability of Fish Production Through Use of Feed Additives Use of Novel Feed Ingredients Reduced Imports & Environmental Impacts
  • 8.
    Use of FermentationTechnology for the Production of Essential Nutrients & Feed Additives Vitamins, pigments, antioxidants & emulsifiers Minerals, trace elements, salt, limestone, zeolite, chelates Amino acids, nucleotides, feeding attractants, sterols Enzymes, gut modifiers, prebiotics, probiotics, organic acids Immune enhancers, anti-fungal, anti-viral, anti-parasitical Essential oils, binders, growth promoters, hormones, antibiotics
  • 9.
    Improving the utilizationof feed ingredients Use of renewable nutrient sources
  • 10.
    Improving the utilizationof feed ingredients Use of renewable nutrient sources Mannanase α - galactosidase EXOGENOUS MICROBIAL ENZYMES •Improved nutrient digestibility •Improving feed efficiency •Release of trapped nutrients •Breakdown of anti-nutritional factors •Improved gut health •Reducing environmental impacts (N, P) β – glucanase Cellulase Amylase Protease Phytase Xylanase Lipase Micotoxinase
  • 11.
    Indonesia Use of microbialphytases to improve phosphorus digestibility and reduce phosphorus loss to the aquatic environment
  • 12.
    Using amino acidsto reduce fishmeal use Use of renewable nutrient sources AMINO ACIDS PRODUCED BY FERMENTATION •Arginine •Glutamine/MSG •Histidine •Isoleucine •Leucine •Lysine AMINO ACIDS PRODUCED BY FERMENTATI ON •Phenylalanine •Proline •Serine •Threonine •Tryptophan •Valine
  • 13.
    Source: Evonik Traditional salmondiet Modern salmon diet* Move from traditional fishmeal and fish oil based diets to plant-based diets for salmonids Beneficial use of using supplemental limiting amino acids
  • 14.
    Aquatic protein meals& oils 1-25% Fishmeals & oil: wild & farmed Squid meal, krill meal Seaweed meals & products Cultured microbial biomass Terrestrial animal proteins & oils 1-25% Poultry by-products Porcine by-products Ruminant by-products Terrestrial invertebrates Terrestrial plant proteins & oils 1-25% Oilseed protein by-products Cereal protein by-products Pulse protein by-products Other plant proteins Other plant meals & fillers 1-25% Cereal meals & by-products Root meals & extracts Fruit meals & by-products Forage & leaf meals Feed additives 0-5% Vitamins, antioxidants, pigments & emulsifiers Minerals, trace elements, salt, zeolites Amino acids, nucleotides, feeding attractants, sterols Gut modifier, prebiotics, probiotics, acidifiers, essential oils Immune enhancers, anti-fungal, anti-viral, anti-parasitical Binders, growth promoters, hormones, antibiotics Major ingredient groups commonly used in AQUACULTURE FEEDS Major ingredient groups commonly used in AQUACULTURE FEEDS
  • 16.
  • 17.
  • 19.
    Alltech, Brasil DV Aquais a complex combination of fermentation metabolites and residual yeast cells Mannan Oligosaccharides Highly branched β- glucans Nucleotides Fermentation metabolites ' ' '''''' ' ' ' Plasma''membrane' (From'Netea'et'al''2008)' β
  • 20.
    Aurantiochytrium – Contains morethan 10 % DHA on a dry weight basis and might be a good source for fish oil replacement in formulated diets. Heliae © 2015 – Confidential 20
  • 21.
    Alltech Algae, Winchester,Kentucky Heterotrophic algae - Schizochytrium 70% Fat containing 28% DHA Source: Alltech
  • 22.
    Objective: More sustainablefish feeds Traditional salmon diet Modern salmon diet* Use of dried biomass of micro algae as fish oil replacer Source: Evonik
  • 24.
    PROBIOTICS 700 ha shrimpfarm Water, Pond Bottom, Feed.. Value added – processing on site A probiotic is defined as: - a live microbial adjunct - which has a beneficial effect on the host - by modifying the host-associated or ambient microbial community - by ensuring improved use of the feed - or enhancing its nutritional value - by enhancing the host response towards disease - or by improving the quality of its ambient environment Verschuere et al. 2000. Microbiology and Molecular biology Reviews 64:655–671 Definition of aquaculture probiotics
  • 25.
    Ojai - Mexico Fermentationof Feed Ingredients to increase nutritional value
  • 26.
  • 27.
    Use of SolidState Fermentation to better utilize locally available agricultural feed & food wastes, & for the reduction of the anti-nutritional factors present in plant proteins such as soybean, rapeseed, lupin & pea
  • 28.
    Fish Matters: Roleof Biotechnology in Improving Nutrition Role of Biotechnology in Improving Conservation & Nutrient Content of Fish for Human Consumption Through Fermentation – Fish Sauce Use of Algal-DHA & Chelated-Minerals
  • 29.
    Garum: Fermented FishSauce for the Ancient Roman Masses Fish fermentation allowed the ancient Romans to store their fish surplus for long periods. Making garum was simple; placing fish (mackerel, sardines, anchovies, or entrails) in a barrel with salt at a 5:1 ratio, placing a weight on top of the mixture, and then allowing to ferment for 2-3 months. By this time the fish will ferment and liquify, creating an umami flavor similar to that of parmesan, and a slightly pungent smell. The liquid can then be removed, and the remaining residue used to make a second batch of fish sauce.
  • 30.
    Wood vats linethe Khai Hoan fish sauce factory, where the fish are fermented
  • 33.
  • 34.
    Microalgae: Haematococcus pluvialis Astaxanthin– natural antioxidant Astaxanthin is 3 % DW
  • 35.
  • 36.
    NUTRITION is thecornerstone that effects the Health & Wellbeing of all People: both Rich & Poor
  • 37.
    JAPAN USA Calories (kcal/day)2,719 3,639 +++ Animal protein (g/day) 49.1 70.7 +++ Animal fats (g/day) 33.9 68.3 +++ Terrestrial meat (kg/year) 48.8 117.6 +++ Fish & seafood (kg/year) 53.7 +++ 21.6 Aquatic animal fat (g/day) 6.14 +++ 1.42 Aquatic plants (kg/year) 0.99 +++ 0 Per capita food supply in Japan & USA (FAO Food Balance Sheets, 2016) it is not by chance that Japan, the country with the highest reported life expectancy and with one of the world’s lowest incidences of obesity & deaths from heart related illnesses, is also one of the worlds top consumer of aquatic animal products & farmed aquatic plants: comparison made with the USA
  • 38.
    • AQUATIC FOODPRODUCTS represent one of the world’s most nutritious & healthy foods • According to FAO/WHO Joint Expert Consultation on the Risks & Benefits of Fish Consumption, there is convincing evidence that  Fish consumption reduces the risk of death from coronary heart disease & consumption by women reduces the risk of suboptimal neurodevelopment in their offspring;  There is also emerging, possible or probable evidence, that fish consumption may also reduce the risk of ischaemic stroke, non-fatal coronary heart disease events, congestive heart failure, atrial fibrillation, cognitive decline, depression, anxiety & inflammatory diseases
  • 39.
    NUTRITIONAL COMPOSITION OFAQUATIC FOODS Compared with terrestrial farmed meat products, aquatic animal & plant foods (whether captured or cultured) are: •Rich source of high quality animal protein with a mean of 17.3% compared with 13.8% for terrestrial meat; •Rich source of long chain omega-3 fatty acids - DHA; •Rich source of essential minerals, including Calcium, Phosphorus, Sodium, Magnesium, Iron, Zinc, Manganese, Copper, Iodine, Chromium, Fluorine; •Rich source of essential vitamins, including Vitamin A, Vitamin D, Vitamin B12, Folic acid, Vitamin E, Choline
  • 40.
    Aquatic plants Cephlapods frozen Molluscsfrozen Crustaceans frozen Marine fish nes fillet Pelagic fish fillet Demersal fish fillet Freshwater/diadromous fish fillet Cows milk Hens egg Poultry meat Turkey meat Pig meat Muttton & lamb Duck meat Chicken meat Beef boneless g/100g Composition of different purchased foods (FAO, 2001 ) Mainly polyunsaturated omega-3 fatty acids Mainly saturated omega-6 fatty acids
  • 41.

Editor's Notes

  • #10 As in more traditional farming systems: diversified formulations to cost-effectively explore several nutritional resources Use of renewable raw materials whose availability can follow predicted aquaculture growth trends
  • #11 As in more traditional farming systems: diversified formulations to cost-effectively explore several nutritional resources Use of renewable raw materials whose availability can follow predicted aquaculture growth trends
  • #13 As in more traditional farming systems: diversified formulations to cost-effectively explore several nutritional resources Use of renewable raw materials whose availability can follow predicted aquaculture growth trends
  • #22 Innovation