Heart lab pharma 1 lab 7 (1) (1).ppt

Heart lab pharma 1 lab 7 (1) (1).ppt

• 1.
  • Pharmacology 1 (3443) • Spring2020 • Lab 7 Faculty of Pharmacy Department of Pharmacology & Toxicology
• 2.
  Determination of thesite of action of Different cardiac stimulant & depressant drugs using Isolated Frog Heart
• 3.
  M2 NN NN 1 Drugs Depressants Stimulants Nn agonist(e.g. Dil. nicotine) β-agonist (e.g. Isoprenaline) M agonist (e.g. Ach) Direct stimulant (e.g. Ca2+) Direct depressant (e.g. K+ )
• 4.
  Setup
• 5.
  -ve chronotropic -ve chronotropic -veinotropic -ve inotropic Depressants Stimulants +ve chronotropic +ve chronotropic +ve inotropic +ve inotropic
• 6.
  Agonists and blockersused in the experiment Receptor Agonist Blocker β Isoprenaline Propranolol Nn Dil. nicotine Hexamethonium M Ach Atropine
• 7.
  Steps to testif a drug acts on a certain receptor UNK AGONIST Before self WASH Before self WASH AGONIST Allow self WASH BLOCKER Allow self WASH Start If no action  Unk acts on the blocked receptor If there is still action  Unk doesn’t act on the blocked receptor  Test another receptor
• 8.
  Why is theagonist added after the blocker? To test for complete blockade of the receptor. Why is the agonist added before the blocker? To test for the sensitivity of the receptor to the added agonist.
• 9.
  PROCEDURE 1. Inject theunk drug 2. Determine the lag time & self washing time
• 10.
  a) If theunk is stimulant: - Add Isoprenaline (allow self washing) - Add the following 3 consecutively (before self washing) No stimulation  Unk is β agonist Stimulation  Unk is directly acting Prop Isop Unk
• 11.
  Unk is DIRECT Unk is β1 agonist UnkIsop Prop Isop Unk
• 12.
  b) If theunknown is depressant:  Test for nicotinic action: - Add dil. Nicotine - Add the following 3: No depression  Unk is Nn agonist Depression  continue the experiment Hexa. Dil. nic. Unk
• 13.
   Test formuscarinic action: - Add Ach - Add the following 3: No depression  Unk is M agonist Depression  Unk is directly acting Atrop. Ach Unk
• 14.
  0.1 Dil. Nic. 0.1 Hexam. 0.1 Dil. Nic 0.1 Unk 0.1 Unk Siteof action: Nicotinic agonist Unknown may be Dil nicotine
• 15.
  0.1 Dil. Nic. 0.2 Hexam. 0.1 Dil. Nic. 0.1 Unk 0.1 Unk 0.1 ACh 0.1 Atrop 0.1 ACh0.1 Unk Site of action: Muscarinic agonist Unknown may be ACh
• 16.
  0.1 Ach 0.1 Hexam. 0.1 Atrop 0.1 Unk 0.1 Dil. Nic. 0.1 Dil. Nic. 0.1 Unk 0.1 ACh0.1 Unk Site of action: Direct acting Depressant Unknown may be K+