JUnit is a unit testing framework for Java that originated from xUnit. It allows developers to write test cases that test classes and methods in Java code. JUnit provides classes like TestCase that define test methods, TestSuite that collects multiple test cases, and TestRunner that executes the tests and reports results. Developers create test classes that extend TestCase and define test methods to verify the behavior of the classes under test. TestRunner is used to run test cases and test suites and display the results, which helps ensure code quality during refactoring.