Laboratory water
purification
Water quality and application
Paul Fowler
Export Manager
2
Contents
• Introduction to Purite
• Water Standards and application
• Technology
• Purite Equipment
3
• Purite designs, develops and manufactures
water purification systems
• For use in applications in laboratory,
healthcare and industry.
• We specialise in a wide range of technologies,
including:
• ion exchange carbon filtration
• reverse osmosis
• electro-deionisation
• ultrafiltration.
About Purite
4
Water Standards
• Water is one of the most important raw materials used for
research & development, laboratory and production
applications.
• Quality of raw water drawn from the mains or local
borehole supplies can be extremely variable.
• Measures need to be in place to purify the water to suitable
standards.
• Specific water purity standards have been developed over
the years.
• Quality of water required is linked to application.
5
Water Standards
BS EN ISO 3696:1995
• Three standardised grades, expressed in terms of conductivity
as below:
Grade Microsiemens(MIN) Resistance(MIN) pH Si(ppb)max
1 0.1 10 N/A 10
2 1 1 N/A 20
3 5 0.2 5-7.5 N/A
6
Water Standards
ASTM D1193-06 (2011)
• Four standardised types, expressed in terms of conductivity as
below. Further graded on bacterial, Endotoxin requirement as
more application specific:
Type Grade Microsiemens(MIN) Resistance(MIN) pH Si(ppb)max cfu/ml max Endotoxins Eu/ml max
I 0.056 18 3
I A 0.056 18 3 10/1000 0.03
I B 0.056 18 3 10/100 0.25
I C 0.056 18 3 100/10
II 1.000 1 3
II A 1.000 1 3 10/1000 0.03
II B 1.000 1 3 10/100 0.25
II C 1.000 1 3 100/10
III 0.250 4 500
III A 0.250 4 500 10/1000 0.03
III B 0.250 4 500 10/100 0.25
III C 0.250 4 500 1000/100
IV 5.000 0.2 5-8
IV A 5.000 0.2 5-8 10/1000 0.03
IV B 5.000 0.2 5-8 10/100 0.25
IV C 5 0.2 5-8 100/10
Most people refer to ASTM standards, but interpretation varies
7
Water Standards
• Other specialised standards exist for industries such as the
electronics and semiconductor industry where specific metal
specifications need to be met.
• The healthcare industry also has its own set of standards.
8
Applications
• Type IV
 Non-Critical work. Water baths, autoclave and
disinfector feed, environmental chambers, plant growth
rooms. Type 1 production.
• Type II/III
 General Lab use. Glassware rinsing, media preparation,
pH solutions & buffers, reagent preparation. Type 1
production.
• Type I
 Water critical applications. Clinical Analysers, HPLC,
GC, AA, ICP-MS, advanced analytical techniques,
Tissue Culture, Life Science, Molecular Biology.
QUALITY - LINKED TO APPLICATION
9
Technology-RO
• Latest RO technology can improve water quality and lab
productivity.
• RO is a powerful method of producing purified water for a
wide range of laboratory duties.
• Modern units will pre-filter and de-chlorinate the raw
water feed, then pass through semi permeable polymer
RO membranes.
• The permeate passes through the membrane which
removes up to 98% of the minerals and salts, plus
virtually all colloids, microorganisms, endotoxins and
organic macromolecules.
10
Technology
Deionisation
• Deionisation can be used purify water.
• It uses a mixture of resins to remove anionic and cationic
contaminants from the feedwater, exchanging with active
hydrogen and hydroxyl ions, which combine to form water
molecules.
• Deionisation can be used alone to produce Grade 1 (>10
MΩ) water, but is uneconomic at high TDS levels or for high
usage (>100l/day).
• Generally the most efficient treatment is a combination of
RO and Deionisation.
11
Technology
Laboratory systems
• Specification should begin with consideration of the
standardised grades of water purity.
• Having established the level of purity required, then the
volume needed should be assessed.
• This is followed by the decision to install a centralised or
stand-alone unit.
• Choice is dictated by overall costs and the operational
demands of each option.
• Purite has developed a range of stand-alone units designed
to meet the needs of the modern laboratory
12
Quality Considerations?
QUALITY - LINKED TO APPLICATION
• RO WATER (5 – 35 µs) – Feed for Humidifiers, Autoclaves,
Lab ring mains.
• DI WATER (1 – 10 MΩ) – Glassware rinsing, Reagent make-
up, Media prep, General Lab use.
• HPLC GRADE WATER (>10 MΩ) – HPLC, Ion
Chromatography, Atomic Adsorption, Analyser feed.
• ULTRA PURE WATER – (18.2 MΩ) – Tissue Culture, Life
Science, Molecular Biology.
13
Equipment
 Labwater
 Descale
 Analyst
 HP
 Purewater 300
 Fusion
 Neptune
14
Pure Water Guide
15
Labwater Range
L3G EDI Variants
 Low volume users
 1-10l/d
 Schools
 Dentists
 Grade 2 (1-10 µs)
16
Labwater 1 & 2 Cartridges
LABWATER 1
• 1 – 10 µs
• 160 Litre apacity
• Colour exchange
indication
• Optional dispense
gun
LABWATER 2
• 1 – 10 µs
• 320 litre capacity
• Colour exchange
indication
• Optional dispense
gun
17
Select Range
Nomenclature
• Descale - Base RO Unit with
pre-treatment
• Analyst - Descale & DI
• HP - Analyst with UMF &
Recirculation
18
Configurations
19
Select Range
• 40 – 3.6 litres / hour
• 80 – 7.2 litres / hour
• 160 – 14.4 litres / hour
• 320 – 30 litres / hour
* Size based on daily usage
per a 10 hour working day
20
Select Descale
• Applications
− Washing machines
− Rinsing
− Humidifier feed
− Autoclave feed
• Configuration
− Carbon Pre-treatment
− Reverse Osmosis
− Integrally Close Coupled
(ICC) or external tank
21
Output Quality
22
Select Analyst
• Applications
− Reagent make up
− Media preparation
− Atomic Adsorption
− Glassware Rinsing
• Configuration
− Carbon Pretreatment
− Reverse Osmosis
− Deionising Resin
− ICC or external tank
23
Output Quality
24
Purewater 300
• Applications
− Laboratory glassware
washing
− 20lt internal tank
− Dishwasher connection
− >1MΩ.cm
• Configuration
− Carbon Pre-treatment
− Reverse Osmosis
− Deionising Resin
− ICC Tank
25
Output Quality
26
Select HP
• Applications
− Ion HPLC
− Chromatography
− Tissue Culture
− Fluorescence
• Configuration
− Carbon Pretreatment
− Reverse Osmosis
− Deionising Resin
− Recirculation Loop
− 0.1µm internal bacterial
filter
− 0.2µm POU filter
− ICC or External Tank
27
Output Quality
28
Select Fusion
• Applications
− Dual quality water
− Analytical >1MΩ.cm
− Life Science upto18.2MΩ.cm
− UPW up to 50lt/day max
− 40, 80, 160, 320 variants
− Bacteria <0.1CFU/ml, TOC
<5ppb
− Endotoxin <0.001EU/ml
• Configuration
− Carbon Pretreatment
− Reverse Osmosis
− Deionising Resin
− Recirculation Pump
− Nuclear Resin Polish
− Ultrafiltration
− Dual UV treatment
185/254nm
− 0.2µm POU filter
29
Fusion Configuration
NB - Carbon Filter External to Unit
30
Fusion Output Quality
31
Tank Options
• Internal 20lt Tank
− 40
− 80
− 160
− 320
• External Tank
− 50lts - 40, 80, 160
− 100lts - 320
32
Neptune – 3 Variants
• Applications
− Guaranteed 18.2MΩ.cm
− Analytical
− Life Science
− Ultimate
− Needs a pretreated water
supply
− 20µS/cm
− Dispenses 2 litres / minute
33
Configuration
34
Neptune Output Quality
35
Product Selection
Considerations
• What flow rate is required? (litres/hr or litres/day)
• What is the demand pattern? (how much needed at once)
• What quality of water is required? (Grade or Type)
• What is the end use application? (General Lab etc)
• What quality is the feedwater? (TDS)
• What is the mains pressure? (bar or psi)
36
Contact Purite
Purite Ltd
Bandet Way
Thame, Oxon
OX9 3SJ UK
Tel: +44 (0)1844 217141
Fax: +44 (0)1844 218098
Email: mail@purite.com
www.purite.com

Laboratory Water Purification Technology, Products and Water Quality Standards

  • 1.
    Laboratory water purification Water qualityand application Paul Fowler Export Manager
  • 2.
    2 Contents • Introduction toPurite • Water Standards and application • Technology • Purite Equipment
  • 3.
    3 • Purite designs,develops and manufactures water purification systems • For use in applications in laboratory, healthcare and industry. • We specialise in a wide range of technologies, including: • ion exchange carbon filtration • reverse osmosis • electro-deionisation • ultrafiltration. About Purite
  • 4.
    4 Water Standards • Wateris one of the most important raw materials used for research & development, laboratory and production applications. • Quality of raw water drawn from the mains or local borehole supplies can be extremely variable. • Measures need to be in place to purify the water to suitable standards. • Specific water purity standards have been developed over the years. • Quality of water required is linked to application.
  • 5.
    5 Water Standards BS ENISO 3696:1995 • Three standardised grades, expressed in terms of conductivity as below: Grade Microsiemens(MIN) Resistance(MIN) pH Si(ppb)max 1 0.1 10 N/A 10 2 1 1 N/A 20 3 5 0.2 5-7.5 N/A
  • 6.
    6 Water Standards ASTM D1193-06(2011) • Four standardised types, expressed in terms of conductivity as below. Further graded on bacterial, Endotoxin requirement as more application specific: Type Grade Microsiemens(MIN) Resistance(MIN) pH Si(ppb)max cfu/ml max Endotoxins Eu/ml max I 0.056 18 3 I A 0.056 18 3 10/1000 0.03 I B 0.056 18 3 10/100 0.25 I C 0.056 18 3 100/10 II 1.000 1 3 II A 1.000 1 3 10/1000 0.03 II B 1.000 1 3 10/100 0.25 II C 1.000 1 3 100/10 III 0.250 4 500 III A 0.250 4 500 10/1000 0.03 III B 0.250 4 500 10/100 0.25 III C 0.250 4 500 1000/100 IV 5.000 0.2 5-8 IV A 5.000 0.2 5-8 10/1000 0.03 IV B 5.000 0.2 5-8 10/100 0.25 IV C 5 0.2 5-8 100/10 Most people refer to ASTM standards, but interpretation varies
  • 7.
    7 Water Standards • Otherspecialised standards exist for industries such as the electronics and semiconductor industry where specific metal specifications need to be met. • The healthcare industry also has its own set of standards.
  • 8.
    8 Applications • Type IV Non-Critical work. Water baths, autoclave and disinfector feed, environmental chambers, plant growth rooms. Type 1 production. • Type II/III  General Lab use. Glassware rinsing, media preparation, pH solutions & buffers, reagent preparation. Type 1 production. • Type I  Water critical applications. Clinical Analysers, HPLC, GC, AA, ICP-MS, advanced analytical techniques, Tissue Culture, Life Science, Molecular Biology. QUALITY - LINKED TO APPLICATION
  • 9.
    9 Technology-RO • Latest ROtechnology can improve water quality and lab productivity. • RO is a powerful method of producing purified water for a wide range of laboratory duties. • Modern units will pre-filter and de-chlorinate the raw water feed, then pass through semi permeable polymer RO membranes. • The permeate passes through the membrane which removes up to 98% of the minerals and salts, plus virtually all colloids, microorganisms, endotoxins and organic macromolecules.
  • 10.
    10 Technology Deionisation • Deionisation canbe used purify water. • It uses a mixture of resins to remove anionic and cationic contaminants from the feedwater, exchanging with active hydrogen and hydroxyl ions, which combine to form water molecules. • Deionisation can be used alone to produce Grade 1 (>10 MΩ) water, but is uneconomic at high TDS levels or for high usage (>100l/day). • Generally the most efficient treatment is a combination of RO and Deionisation.
  • 11.
    11 Technology Laboratory systems • Specificationshould begin with consideration of the standardised grades of water purity. • Having established the level of purity required, then the volume needed should be assessed. • This is followed by the decision to install a centralised or stand-alone unit. • Choice is dictated by overall costs and the operational demands of each option. • Purite has developed a range of stand-alone units designed to meet the needs of the modern laboratory
  • 12.
    12 Quality Considerations? QUALITY -LINKED TO APPLICATION • RO WATER (5 – 35 µs) – Feed for Humidifiers, Autoclaves, Lab ring mains. • DI WATER (1 – 10 MΩ) – Glassware rinsing, Reagent make- up, Media prep, General Lab use. • HPLC GRADE WATER (>10 MΩ) – HPLC, Ion Chromatography, Atomic Adsorption, Analyser feed. • ULTRA PURE WATER – (18.2 MΩ) – Tissue Culture, Life Science, Molecular Biology.
  • 13.
    13 Equipment  Labwater  Descale Analyst  HP  Purewater 300  Fusion  Neptune
  • 14.
  • 15.
    15 Labwater Range L3G EDIVariants  Low volume users  1-10l/d  Schools  Dentists  Grade 2 (1-10 µs)
  • 16.
    16 Labwater 1 &2 Cartridges LABWATER 1 • 1 – 10 µs • 160 Litre apacity • Colour exchange indication • Optional dispense gun LABWATER 2 • 1 – 10 µs • 320 litre capacity • Colour exchange indication • Optional dispense gun
  • 17.
    17 Select Range Nomenclature • Descale- Base RO Unit with pre-treatment • Analyst - Descale & DI • HP - Analyst with UMF & Recirculation
  • 18.
  • 19.
    19 Select Range • 40– 3.6 litres / hour • 80 – 7.2 litres / hour • 160 – 14.4 litres / hour • 320 – 30 litres / hour * Size based on daily usage per a 10 hour working day
  • 20.
    20 Select Descale • Applications −Washing machines − Rinsing − Humidifier feed − Autoclave feed • Configuration − Carbon Pre-treatment − Reverse Osmosis − Integrally Close Coupled (ICC) or external tank
  • 21.
  • 22.
    22 Select Analyst • Applications −Reagent make up − Media preparation − Atomic Adsorption − Glassware Rinsing • Configuration − Carbon Pretreatment − Reverse Osmosis − Deionising Resin − ICC or external tank
  • 23.
  • 24.
    24 Purewater 300 • Applications −Laboratory glassware washing − 20lt internal tank − Dishwasher connection − >1MΩ.cm • Configuration − Carbon Pre-treatment − Reverse Osmosis − Deionising Resin − ICC Tank
  • 25.
  • 26.
    26 Select HP • Applications −Ion HPLC − Chromatography − Tissue Culture − Fluorescence • Configuration − Carbon Pretreatment − Reverse Osmosis − Deionising Resin − Recirculation Loop − 0.1µm internal bacterial filter − 0.2µm POU filter − ICC or External Tank
  • 27.
  • 28.
    28 Select Fusion • Applications −Dual quality water − Analytical >1MΩ.cm − Life Science upto18.2MΩ.cm − UPW up to 50lt/day max − 40, 80, 160, 320 variants − Bacteria <0.1CFU/ml, TOC <5ppb − Endotoxin <0.001EU/ml • Configuration − Carbon Pretreatment − Reverse Osmosis − Deionising Resin − Recirculation Pump − Nuclear Resin Polish − Ultrafiltration − Dual UV treatment 185/254nm − 0.2µm POU filter
  • 29.
    29 Fusion Configuration NB -Carbon Filter External to Unit
  • 30.
  • 31.
    31 Tank Options • Internal20lt Tank − 40 − 80 − 160 − 320 • External Tank − 50lts - 40, 80, 160 − 100lts - 320
  • 32.
    32 Neptune – 3Variants • Applications − Guaranteed 18.2MΩ.cm − Analytical − Life Science − Ultimate − Needs a pretreated water supply − 20µS/cm − Dispenses 2 litres / minute
  • 33.
  • 34.
  • 35.
    35 Product Selection Considerations • Whatflow rate is required? (litres/hr or litres/day) • What is the demand pattern? (how much needed at once) • What quality of water is required? (Grade or Type) • What is the end use application? (General Lab etc) • What quality is the feedwater? (TDS) • What is the mains pressure? (bar or psi)
  • 36.
    36 Contact Purite Purite Ltd BandetWay Thame, Oxon OX9 3SJ UK Tel: +44 (0)1844 217141 Fax: +44 (0)1844 218098 Email: [email protected] www.purite.com