Microorganisms play important roles in food as pathogens that can cause disease, as spoilage organisms that degrade food quality, and as cultured microbes used in food processing. Fruits and vegetables naturally contain saprophytic bacteria and fungi that can lead to spoilage when the protective barriers are broken during harvesting or processing. The major factors affecting microbial growth in fruits and vegetables include physical damage, pH, moisture content, and temperature. Common preservation methods include drying, freezing, acidification, and use of preservatives.