Download to read offline
More Related Content
Similar to MSC IV SEMESTER_DNA Profiling - CE.pdf
MSC IV SEMESTER_DNA Profiling - CE.pdf
- 1.
- 8.
- 24. Glass capillaries withviscous polymer
- 30.
- 32. Sample Preparation PCR productsdilution 1 in 10 with deionized formamide Reduce salt conc, denaturation of dsDNA, aid in electrokinetic injection process “snap-cooling” : 95 degree C for several minutes followed by rapid cooling to around 4 degree Celsius Hi-Di formamide (Applied Biosystems, Foster City, CA)
- 33. DNA SEPARATION Polymer Buffer Capillarytube Electric field / Voltage 15,000 volts Ph 5-8
- 34. POLYMER BUFFER POP-4 andPOP-6, POP-7where “POP” stands for Performance Optimized Polymer. 100mmol/L TAPS and 1mmol/L EDTA, adjusted to pH 8.0 with NaOH TAPS is short for N-tris- (hydroxymethyl)-methyl-3- aminopropane-sulfonic acid. These polymer solutions are 4% and 6% concentrations of linear, uncross- linked dimethyl polyacrylamide, respectively TAPS is used instead of Trisborate- EDTA (TBE) since TBE is temperature and pH-sensitive. A high concentration of urea buffer additives such as formamide, urea, and 2-pyrrolidinone POP-4 consists of 4% linear dimethylacrylamide, 8mol/L urea, 5% 2-pyrrolidinone capillary run temperatures of 60oC
- 35. A number offactors influence resolution in a CE system capillary length separation voltage polymer concentratioN
- 52.
- 53. This Photo byUnknown Author is licensed under CC BY-SA