nanotechnology-and-its-applications-in-medicine-2161-0444-1000247.pdf

Nikalje, Med chem 2015, 5:2
DOI: 10.4172/2161-0444.1000247
Research Article Open Access
Med chem
ISSN: 2161-0444 Med chem, an open access journal
Volume 5(2): 081-089 (2015) - 81
Nanotechnology and its Applications in Medicine
Anna Pratima Nikalje*
Department of Pharmaceutical chemistry, Y.B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad- 431001, Maharashtra, India
*Corresponding author: Department of Pharmaceutical Chemistry, Y.B.
Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh,
Aurangabad- 431001, Maharashtra, India, Tel: +91 9823619992, E-mail:
annapratimanikalje@gmail.com
Received February 16, 2015; Accepted March 10, 2015; Published March 12,
2015
Citation: Nikalje AP (2015) Nanotechnology and its Applications in Medicine. Med
chem 5: 081-089. doi:10.4172/2161-0444.1000247
Copyright: © 2015 Nikalje AP. This is an open-access article distributed under
the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and
source are credited.
Keywords: Nano devices; Nano material; Nano medicine; Nano
pharmaceutics; Drug delivery
Introduction
Advancement in the field of nanotechnology and its applications
to the field of medicines and pharmaceuticals has revolutionized the
twentieth century. Nanotechnology [1] is the study of extremely small
structures. The prefix “nano” is a Greek word which means “dwarf”.
The word “nano” means very small or miniature size. Nanotechnology
is the treatment of individual atoms, molecules, or compounds into
structures to produce materials and devices with special properties.
Nanotechnology involve work from top down i.e. reducing the size of
large structures to smallest structure e.g. photonics applications in nano
electronics and nano engineering, top-down or the bottom up, which
involves changing individual atoms and molecules into nanostructures
and more closely resembles chemistry biology.
Nanotechnology deals with materials in the size of 0.1 to 100
nm; however it is also inherent that these materials should display
different properties such as electrical conductance chemical reactivity,
magnetism, optical effects and physical strength, from bulk materials as
a result of their small size.
Nanotechnology works on matter at dimensions in the nanometer
scale length (1-100 nm), and thus can be used for a broad range of
applications and the creation of various types of nano materials and
nano devices.
History of Nanotechnology
The development in the field of nanotechnology started in 1958 and
the various stages of development have been summarized in Table 1.
Nano scale and Nanostructures
The nano scale is the place where the properties of most common
things are determined just above the scale of an atom. Nano scale objects
have at least one dimension (height, length, depth) that measures
between 1 and 999 nanometers (1-999 nm) (Figure 1).
The brief explanation of pharmaceutical nano system is as follows:
As shown in the schematic diagram (Figure 2), pharmaceutical
nanotechnology is divided in two basic types of nano tools viz. nano
materials and nano devices. These materials can be sub classified into
nano crystalline and nano structured materials. Nano structure consists
of nano particles, dendrimers, micelles, drug conjugates, metallic nano
particles etc.
Carbon nano tubes: These are small macromolecules that are unique
Abstract
Nanotechnology is the study of extremely small structures, having size of 0.1 to 100 nm. Nano medicine is a relatively
new field of science and technology. Brief explanation of various types of pharmaceutical nano systems is given.
Classification of nano materials based on their dimensions is given. An application of Nanotechnology in various fields
such as health and medicine, electronics, energy and environment, is discussed in detail. Applications of nano particles in
drug delivery, protein and peptide delivery, cancer are explained. Applications of various nano systems in cancer therapy
such as carbon nano tube, dendrimers, nano crystal, nano wire, nano shells etc. are given. The advancement in nano
technology helps in the treatment of neuro degenerative disorders such as Parkinson’s disease and Alzheimer’s disease.
Applications of nano technology in tuberculosis treatment, the clinical application of nanotechnology in operative dentistry,
in ophthalmology, in surgery, visualization, tissue engineering, antibiotic resistance, immune response are discussed in
this article. Nano pharmaceuticals can be used to detect diseases at much earlier stages.
Figure 1: Nanoscale and nanostructures.
Figure 2: Schematic diagram of various types of pharmaceutical nano systems.
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Citation: Nikalje AP (2015) Nanotechnology and its Applications in Medicine. Med chem 5: 081-089. doi:10.4172/2161-0444.1000247
importance for biomedical use (Figure 3).
Liposomes: These have been extensively explored and most
developed nano carriers for novel and targeted drug delivery due to
their small size, these are 50-200 nm in size. When dry phospholipids
are hydrated, closed vesicles are formed (Figure 4). Liposomes are
biocompatible, versatile and have good entrapment efficiency. It finds
application as long circulatory and in passive and active delivery of
gene, protein and peptide.
Dendrimers: Dendrimers are hyper branched, tree-like structures.
It contains three different regions: core moiety, branching units, and
closely packed surface (Figure 5). It has globular structure and encloses
internal cavities. Its size is less than 10 nm. These are used for long
for their size, shape, and have unique physical properties. Nano tubes
have some special advantages over other drug delivery and diagnostic
systems (Figure 3) due to their unique physical properties.
Metallic nano particles: Metallic nano particles have used in
drug delivery, especially in treatment of cancer and also in biosensors.
Amongst various metals, silver and gold nano particles are of prime
Year Development in nanotechnology
1959 R. Feynman initiated thought process
1974 The term nanotechnology was used by Taniguchi for the first time.
1981 IBM Scanning Tunneling Microscope
1985 “Bucky Ball”
1986 First book on nanotechnology Engines of Creation published by K. Eric Drexler, Atomic Force Microscope
1989 IBM logo was made with individual atoms
1991 S. Iijima discovered Carbon Nano tube for the first time.
1999
1st nano medicine book by R. Freitas “Nano medicine” was published
2000 For the first time National Nanotechnology Initiative was launched
2001
For developing theory of nanometer-scale electronic devices and for synthesis and characterization of carbon nanotubes and nano wires, Feynman Prize
in Nanotechnology was awarded
2002
Feynman Prize in Nanotechnology was awarded for using DNA to enable the self-assembly of new structures and for advancing our ability to model
molecular machine systems.
2003
Feynman Prize in Nanotechnology was awarded for modeling the molecular and electronic structures of new materials and for integrating single molecule
biological motors with nano-scale silicon devices.
2004
First policy conference on advanced nanotech was held. First center for nano mechanical systems was established, Feynman Prize in Nanotechnology was
warded for designing stable protein structures and for constructing a novel enzyme with an altered function.
2005-2010 3D Nano systems like robotics, 3D networking and active nano products that change their state during use were prepared.
2011 Era of molecular nano technology started
Table 1: Periodical development in nanotechnology.
Figure 3: Surface functionalized gold nano particles.
Figure 4: Structure of Liposomes.

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Volume 5(2): 081-089 (2015) - 83
circulatory, controlled delivery of bioactive material, targeted delivery
of bioactive particles to macrophages and liver targeted delivery.
Classification of Nano Materials
Nano materials can be classified dimension wise into following
categories:
Classification Examples
• Nano rods, nano wires have dimension less than 100 nm.
• Tubes, fibers, platelets have dimensions less than 100 nm.
• Particles, quantum dots, hollow spheres have 0 or 3 Dimensions
< 100 nm.
On the basis of phase composition, nano materials in different
phases can be classified as,
• The nano material is called single phase solids. Crystalline,
amorphous particles and layers are included in this class.
• Matrix composites, coated particles are included in multi-phase
solids.
• Multi-phase systems of nano material include colloids, aero
gels, Ferro fluids, etc.
Manufacturing Approaches
The two major approaches [2] to get nano materials are -one is the
bottom up and the other is top down approach. Bottom up produce
components which are made of single molecules, and covalent forces
hold them together that are far stronger than the forces that hold
together macro-scale components. Enormous amount of information
could be stored in devices build from the bottom up. For example,
use of AFM, liquid phase techniques based on inverse micelles, sol-
gel processing, and chemical vapor deposition (CVD), laser pyrolysis
and molecular self-assembly use bottom up approach for nano scale
material manufacturing.
Top manufacturing involves the construction of parts through
methods such as cutting, carving and molding and due to our
limitations in these processes highly advanced nano devices are yet to be
manufactured. Laser ablation, milling, nano-lithography, hydrothermal
technique, physical vapor deposition and electrochemical method
(electroplating) uses top down approach for nano-scale material
manufacturing.
Every element of periodic table can be utilized in nanotechnology
depending upon the target material which someone is going to fabricate
range from nano medicine and goes up to nano concrete via nano
electronics. Nanotechnology provides us the chance to synthesize nano
scale building blocks with control on size, composition etc. Materials
manufacturing will be revolutionized by further assembling into larger
structures with designed properties. Without machining, metals,
polymers, ceramics etc. can be manufactured at exact shape.
Nanotechnology can benefit chemical catalysis due to the extremely
large surface to volume ratio. The various applications of nanoparticles
in catalysis range from fuel cell to catalytic converters and photocatalytic
devices. It is also important for the production of chemicals. Modern
revolution in catalysis is due to the availability of unlimited commercial
quantities of zeolites.
Applications of Nanotechnology
Thedifferentfieldsthatfindpotentialapplicationsofnanotechnology
are as follows:
a. Health and Medicine
b. Electronics
c. Transportation
d. Energy and Environment
e. Space exploration
Nanotechnology in health and medicine
Even today various disease like diabetes, cancer, Parkinson’s
disease, Alzheimer’s disease, cardiovascular diseases and multiple
sclerosis as well as different kinds of serious inflammatory or infectious
diseases (e.g. HIV) constitute a high number of serious and complex
illnesses which are posing a major problem for the mankind. Nano-
medicine is an application of nanotechnology which works in the field
of health and medicine. Nano-medicine makes use of nano materials,
and nano electronic biosensors. In the future, nano medicine will
benefit molecular nanotechnology. The medical area of nano science
application has many projected benefits and is potentially valuable for
all human races.
With the help of nano medicine early detection and prevention,
improved diagnosis, proper treatment and follow-up of diseases is
possible. Certain nano scale particles are used as tags and labels,
biological can be performed quickly, the testing has become more
sensitive and more flexible. Gene sequencing has become more efficient
with the invention of nano devices like gold nano particles, these gold
particles when tagged with short segments of DNA can be used for
detection of genetic sequence in a sample.
With the help of nanotechnology, damaged tissue can be reproduced
or repaired. These so called artificially stimulated cells are used in tissue
engineering, which might revolutionize the transplantation of organs
or artificial implants.
Advanced biosensors with novel features can be developed with
the help of Carbon nano tubes. These biosensors can be used for
astrobiology and can throw light on study origins of life. This technology
is also being used to develop sensors for cancer diagnostics. Though
CNT is inert, it can be functionalized at the tip with a probe molecule.
Their study uses AFM as an experimental platform.
i. Probe molecule to serve as signature of leukemia cells identified.
ii. Current flow due to hybridization will be through CNT electrode
to an IC chip.
iii. Prototype biosensors catheter development.
Nanotechnology has made excellent contribution in the field
Figure 5: Schematic representation of a dendrimers showing core, branches,
and surface.

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Volume 5(2): 081-089 (2015) - 84
of stem cell research. For example, magnetic nanoparticles (MNPs)
have been successfully used to isolate and group stem cells. Quantum
dots have been used for molecular imaging and tracing of stem cells,
for delivery of gene or drugs into stem cells, nano materials such as
carbon nano tubes, ﬂuorescent CNTs and ﬂuorescent MNPs have been
used. Unique nanostructures were designed for controllable regulation
of proliferation and differentiation of stem cells is done by designed
unique nano structures. All these advances speed up the development
of stem cells toward the application in regenerative medicine [3]. The
recent applications of nanotechnology in stem cell research promises to
open new avenues in regenerative medicine. Nanotechnology can be a
valuable tool to track and image stem cells, to drive their differentiation
into specific cell lineage and ultimately to understand their biology. This
will hopefully lead to stem cell-based therapeutics for the prevention,
diagnosis and treatment of human diseases [4].
Nano devices can be used in stem cell research in tracking
and imaging them. It has its applications for basic science as well as
translational medicine. Stem cells can be modulated by mixing of nano
carriers with biological molecules (Figure 6). Nano devices can be used
for intracellular access and also for intelligent delivery and sensing
of biomolecules. These technologies have a great impact in stem cell
microenvironment and tissue engineering studies and have a great
potential for biomedical applications [5].
Nanotechnology, energy and environment
Nanotechnology will play a critical role in coming 50 years by
protecting the environment and providing sufficient energy for a
growing world. The advanced techniques of nanotechnology can
help storage of energy, its conversion into other forms, ecofriendly
manufacturing of materials and by better enhanced renewable energy
sources.
Nanotechnology can be used for less expensive energy production
and for renewal energies, in solar technology, nano-catalysis, fuel cells
and hydrogen technology. Carbon nano tube fuel cells are used for
storage of hydrogen, thus finds application in power cars.
Nanotechnology is used on photovoltaic , for making them cheap,
light weight and more efficient, which can reduce the combustion of
engine pollutants by nano porous filters, and can clean the exhaust
mechanically, with the help of catalytic converters made up of nano
scale noble metal particles and by catalytic coatings on cylinder walls
and catalytic nanoparticles as additive for fuels.
Nanotechnology can help in developing new ecofriendly and green
technologies that can minimize undesirable pollution. Solid state
lightening can reduce total electricity consumption. Nano technological
approaches can lead to a strong reduction of energy consumption for
illumination.
Medical use of Nano Materials
Nano medicine is a relatively new field of science and technology.
By interacting with biological molecules at nano scale, nanotechnology
broadens the field of research and application. Interactions of nano
devices with bio molecules can be understood both in the extracellular
medium and inside the human cells. Operation at nano scale allows
exploitation of physical properties different from those observed at
micro scale such as the volume/surface ratio.
Two forms of nano medicine that have already been tested
in mice and are awaiting human trials; use of gold nano shells to help
diagnose and cure cancer, and the use of liposome as vaccine adjuvants
and as vehicles for drug transport [6,7]. Similarly, drug detoxification
is also another application for nano medicine which has been used
successfully in rats. Medical technologies can make use of smaller
devices are less invasive and can possibly be implanted inside the body,
and their biochemical reaction times are much shorter. As compared
to typical drug delivery nano devices are faster and more sensitive [8].
Drug Delivery
In nanotechnology nano particles are used for site specific drug
delivery. In this technique the required drug dose is used and side-effects
are lowered significantly as the active agent is deposited in the morbid
region only. This highly selective approach can reduce costs and pain
to the patients. Thus variety of nano particles such as dendrimers, and
nano porous materials find application. Micelles obtained from block
co-polymers,areusedfordrugencapsulation.Theytransportsmalldrug
molecules to the desired location. Similarly, nano electromechanical
systems are utilized for the active release of drugs. Iron nano particles
or gold shells are finding important application in the cancer treatment.
A targeted medicine reduces the drug consumption and treatment
expenses, making the treatment of patients cost effective.
Nano medicines used for drug delivery, are made up of nano scale
particles or molecules which can improve drug bioavailability. For
maximizing bioavailability both at specific places in the body and over a
period of time, molecular targeting is done by nano engineered devices
such as nano robots [9]. The molecules are targeted and delivering
of drugs is done with cell precision. In vivo imaging is another area
where Nano tools and devises are being developed for in vivo imaging.
Using nano particle images such as in ultrasound and MRI, nano
particles are used as contrast. The nano engineered materials are
being developed for effectively treating illnesses and diseases such
as cancer. With the advancement of nanotechnology, self-assembled
biocompatible nano devices can be created which will detect the
cancerous cells and automatically evaluate the disease, will cure and
prepare reports.
The pharmacological and therapeutic properties of drugs can
be improved by proper designing of drug delivery systems, by use
of lipid and polymer based nano particles [10]. The strength of drug
delivery systems is their ability to alter the pharmacokinetics and bio-
distribution of the drug. Nano particles are designed to avoid the body's
defense mechanisms [11] can be used to improve drug delivery. New,
complex drug delivery mechanisms are being developed, which can
get drugs through cell membranes and into cell cytoplasm, thereby
increasing efficiency. Triggered response is one way for drug molecules
to be used more efficiently. Drugs that are placed in the body can activate
only on receiving a particular signal. A drug with poor solubility will
be replaced by a drug delivery system, having improved solubility
Figure 6: Nanotechnology applications in stem cell biology and medicine.

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Volume 5(2): 081-089 (2015) - 85
due to presence of both hydrophilic and hydrophobic environments
[12]. Tissue damage by drug can be prevented with drug delivery, by
regulated drug release. With drug delivery systems larger clearance
of drug from body can be reduced by altering the pharmacokinetics
of the drug. Potential nano drugs will work by very specific and well-
understood mechanisms; one of the major impacts of nanotechnology
and nanoscience will be in leading development of completely new
drugs with more useful behavior and less side effects.
Thus nano particles are promising tools for the advancement of drug
delivery, as diagnostic sensors and bio imaging. The bio-distribution of
these nanoparticles is still imperfect due to the complex host's reactions
to nano- and micro sized materials and the difficulty in targeting
specific organs in the body. Efforts are made to optimize and better
understand the potential and limitations of nano particulate systems.
In the excretory system study of mice dendrimers are encapsulated
for drug deliver of positively-charged gold nano particles, which were
found to enter the kidneys while negatively-charged gold nanoparticles
remained in the important organs like spleen and liver. The positive
surface charge of the nanoparticle decreases the rate of opsonization
of nanoparticles in the liver, thus affecting the excretory pathway. Due
to small size of 5 nm, nano particles can get stored in the peripheral
tissues, and therefore can get collected in the body over time. Thus
nano particles can be used successfully and efficiently for targeting and
distribution, further research can be done on nano toxicity so that its
medical uses can be increased and improved [13].
The applications of nano particles in drug delivery
Abraxane, is albumin bound paclitaxel, a nano particle used
for treatment of breast cancer and non-small- cell lung cancer
(NSCLC). Nano particles are used to deliver the drug with enhanced
effectiveness for treatment for head and neck cancer, in mice model
study ,which was carried out at from Rice University and University
of Texas MD Anderson Cancer Center. The reported treatment uses
Cremophor EL which allows the hydrophobic paclitaxel to be delivered
intravenously. When the toxic Cremophor is replaced with carbon
nano particles its side effects diminished and drug targeting was much
improved and needs a lower dose of the toxic paclitaxel [14].
Nano particle chain was used to deliver the drug doxorubicin to
breast cancer cells in a mice study at Case Western Reserve University.
The scientists prepared a 100 nm long nano particle chain by chemically
linking three magnetic, iron-oxide nano spheres, to one doxorubicin-
loaded liposome. After penetration of the nano chains inside the
tumor magnetic nanoparticles were made to vibrate by generating,
radiofrequency field which resulted in the rupture of the liposome,
thereby dispersing the drug in its free form throughout the tumor.
Tumor growth was halted more effectively by nanotechnology than the
standard treatment with doxorubicin and is less harmful to healthy cells
as very less doses of doxorubicin were used [15,16].
Polyethylene glycol (PEG) nano particles carrying payload
of antibiotics at its core were used to target bacterial infection more
precisely inside the body, as reported by scientists of MIT. The nano
delivery of particles, containing a sub-layer of pH sensitive chains of
the amino acid histidine, is used to destroy bacteria that have developed
resistance to antibiotics because of the targeted high dose and prolonged
release of the drug. Nanotechnology can be efficiently used to treat
various infectious diseases [17,18].
Researchers in the Harvard University Wyss Institute have used
the biomimetic strategy in a mouse model .Drug coated nano particles
were used to dissolve blood clots by selectively binding to the narrowed
regions in the blood vessels as the platelets do [19]. Biodegradable nano
particle aggregates were coated with tissue plasminogen activator, tPA,
were injected intravenously which bind and degrade the blood clots.
Due to shear stresses in the vessel narrowing region dissociation of the
aggregates occurs and releases the tPA-coated nano particles. The nano
therapeutics can be applied greatly to reduce the bleeding, commonly
found in standard thrombosis treatment.
The researchers in the University of Kentucky have created
X-shapedRNAnanoparticles,whichcancarryfourfunctionalmodules.
These chemically and thermodynamically stable RNA molecules are
able of remaining intact in the mouse body for more than 8 hours and to
resist degradation by RNAs in the blood stream. These X-shaped RNA
can be effectively performing therapeutic and diagnostic functions.
They regulate gene expression and cellular function, and are capable of
binding to cancer cells with precision, due to its design [20,21].
‘Minicell’ nano particle are used in early phase clinical trial for
drug delivery for treatment of patients with advanced and untreatable
cancer. The minicells are built from the membranes of mutant bacteria
and were loaded with paclitaxel and coated with cetuximab, antibodies
and used for treatment of a variety of cancers. The tumor cells engulf
the minicells. Once inside the tumor, the anti-cancer drug destroys the
tumor cells. The larger size of minicells plays a better profile in side-
effects. The minicell drug delivery system uses lower dose of drug and
has less side-effects can be used to treat a number of different cancers
with different anti-cancer drugs [22,23].
Nano sponges are important tools [24] in drug delivery, due to
their small size and porous nature they can bind poorly-soluble drugs
within their matrix and improve their bioavailability. They can be made
to carry drugs to specific sites, thus help to prevent drug and protein
degradation and can prolong drug release in a controlled manner.
Proteins and Peptide Delivery
Protein and peptides are macromolecules and are called
biopharmaceuticals. These have been identified for treatment of
various diseases and disorders as they exert multiple biological actions
in human body. Nano materials like nano particles and dendrimers
are called as nano biopharmaceuticals , are used for targeted and/or
controlled delivery.
Applications
Nano particles were found useful in delivering the myelin antigens,
whichinduceimmunetoleranceinamousemodelwithrelapsingmultiple
sclerosis. In this technique, biodegradable polystyrene micro particles
coated with the myelin sheath peptides will reset the mouse’s immune
system and thus prevent the recurrence of disease and reduce the
symptoms as the protective myelin sheath forms coating on the nerve
fibers of the central nervous system. This method of treatment can
potentially be used in treatment of various other autoimmune diseases
[25,26].
Cancer
Due to the small size of nano particles can be of great use
in oncology, particularly in imaging. Nano particles, such as quantum
dots, with quantum confinement properties, such as size-tunable
light emission, can be used in conjunction with magnetic resonance
imaging, to produce exceptional images of tumor sites. As compared
to organic dyes, nano particles are much brighter and need one light
source for excitation. Thus the use of fluorescent quantum dots could
produce a higher contrast image and at a lower cost than organic dyes
used as contrast media. But quantum dots are usually made of quite
toxic elements.

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Nano particles have a special property of high surface area to
volume ratio, which allows various functional groups to get attached to
a nano particle and thus bind to certain tumor cells. Furthermore, the
10 to 100 nm small size of nanoparticles, allows them to preferentially
accumulate at tumor sites as tumors lack an effective lymphatic drainage
system. Multifunctional nano particles can be manufactured that would
detect, image, and then treat a tumor in future cancer treatment [27].
Kanzius RF therapy attaches microscopic nano particles to cancer cells
and then "cooks" tumors inside the body with radio waves that heat
only the nanoparticles and the adjacent (cancerous) cells.
Nano wires are used to prepare sensor test chips, which can detect
proteins and other biomarkers left behind by cancer cells, and detect
and make diagnosis of cancer possible in the early stages from a single
drops of a patient's blood [28].
Nano technology based drug delivery is based upon three facts: i)
efficient encapsulation of the drugs, ii) successful delivery of said drugs
to the targeted region of the body, and iii) successful release of that drug
there.
Nano shells of 120 nm diameter, coated with gold were used
to kill cancer tumors in mice by Prof. Jennifer at Rice University.
These nano shells are targeted to bond to cancerous cells by
conjugating antibodies or peptides to the nano shell surface. Area of
the tumor is irradiated with an infrared laser, which heats the gold
sufficiently and kills the cancer cells [29].
Cadmium selenide nano particles in the form of quantum dots
are used in detection of cancer tumors because when exposed to
ultraviolet light, they glow. The surgeon injects these quantum dots
into cancer tumors and can see the glowing tumor, thus the tumor can
easily be removed.
Nano particles are used in cancer photodynamic therapy, wherein
the particle is inserted within the tumor in the body and is illuminated
with photo light from the outside. The particle absorbs light and
if it is of metal, it will get heated due to energy from the light. High
energy oxygen molecules are produced due to light which chemically
react with and destroy tumors cell, without reacting with other body
cells. Photodynamic therapy has gained importance as a noninvasive
technique for dealing with tumors.
The applications of various nano systems in cancer therapy [30] are
summarized as:
• Carbon nano tubes, 0.5–3 nm in diameter and 20–1000 nm
length, are used for detection of DNA mutation and for detection of
disease protein biomarker.
• Dendrimers, less than 10 nm in size are useful for controlled
release drug delivery, and as image contrast agents.
• Nano crystals, of 2-9.5 nm size cause improved formulation
for poorly-soluble drugs, labeling of breast cancer marker HeR2surface
of cancer cells.
• Nano particles are of 10-1000 nm size and are used in MRI
and ultrasound image contrast agents and for targeted drug delivery,
as permeation enhancers and as reporters of apoptosis, angiogenesis.
• Nano shells find application in tumor-specific imaging, deep
tissue thermal ablation.
• Nano wires are useful for disease protein biomarker
detection, DNA mutation detection and for gene expression detection.
• Quantum dots, 2-9.5 nm in size, can help in optical detection
of genes and proteins in animal models and cell assays, tumor and
lymph node visualization.
Nanotechnology in the treatment of neurodegenerative
disorders
One of the most important applications of nanotechnology is in
the treatment of neuro degenerative disorders [31]. For the delivery of
CNS therapeutics, various nano carriers such as, dendrimers, nano gels,
nano emulsions, liposomes, polymeric nano particles, solid lipid nano
particles, and nano suspensions have been studied. Transportation of
these nano medicines has been effected across various in vitro and in
vivo BBB models by endocytosis and/or transcytosis, and early
preclinical success for the management of CNS conditions such as,
Alzheimer's disease, brain tumors, HIV encephalopathy and acute
ischemic stroke has become possible. The nanomedicine can be
advanced further by improving their BBB permeability and reducing
their neurotoxicity (Figure 7).
Parkinson's disease: This can improve current therapy of
Parkinson's disease (PD). Parkinson's disease (PD) is the second
most common neurodegenerative disease after Alzheimer's disease
and affects one in every 100 persons above the age of 65 years, PD is a
disease of the central nervous system; neuro inflammatory responses
are involved and leads to severe difficulties with body motions. The
present day therapies aim to improve the functional capacity of the
patient for as long as possible but cannot modify the progression of the
neurodegenerative process.
Aim of applied nanotechnology is regeneration and neuro
protection of the central nervous system (CNS) and will significantly
benefit from basic nanotechnology research conducted in parallel with
advances in neurophysiology, neuropathology and cell biology. The
efforts are taken to develop novel technologies that directly or indirectly
help in providing neuro protection and/or a permissive environment
and active signaling cues for guided axon growth. In order to minimize
the peripheral side-effects of conventional forms of Parkinson's disease
therapy, research is focused on the design, biometric simulation and
optimization of an intracranial nano-enabled scaffold device (NESD)
for the site-specific delivery of dopamine to the brain, as a strategy.
Peptides and peptidic nano particles are newer tools for various CNS
diseases.
Nanotechnology will play a key role in developing new diagnostic
and therapeutic tools. Nanotechnology could provide devices to limit
and reverse neuro pathological disease states, to support and promote
functional regeneration of damaged neurons, to provide neuro
protection and to facilitate the delivery of drugs and small molecules
across the blood–brain barrier. For the delivery of CNS therapeutics,
various nanocarriers such as dendrimers, nano gels, nano emulsions,
liposomes, polymeric nano particles, solid lipid nano particles, and
nano suspensions have been studied. Transportation of these nano
Figure 7: Delivery of nano medicine to CNS through BBB.

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Volume 5(2): 081-089 (2015) - 87
medicines has been effected across various in vitro and in vivo BBB
models by endocytosis and/or transcytosis, and early preclinical success
for the management of CNS conditions such as, Alzheimer's disease,
brain tumors, HIV encephalopathy and acute ischemic stroke has
become possible. Future development of CNS nanomedicines needs to
focus on increasing their drug-trafficking performance and specificity
for brain tissue using novel targeting moieties.
Alzheimer's disease: Worldwide, more than 35 million people
are affected by Alzheimer's disease (AD), which is the most common
form dementia. Nano technology finds significant applications in
neurology. These approaches are based on the, early AD diagnosis
and treatment is made possible by designing and engineering of a
plethora of nanoparticulate entities with high specificity for brain
capillary endothelial cells. Nano particles (NPs) have high affinity for
the circulating amyloid-β (Aβ) forms and therefore may induce “sink
effect” and improve the AD condition. In vitro diagnostics for AD has
advanced due to ultrasensitive NP-based bio-barcodes and immune
sensors, as well as scanning tunneling microscopy procedures capable
of detecting Aβ1−40
and Aβ1−42.
The recent research on use of nano
particles in the treatment of Alzheimer’s disease is as shown in Figure
8 [32].
Tuberculosis treatment: Tuberculosis (TB) is the deadly infectious
disease. The long duration of the treatment and the pill burden can
hamper patient lifestyle and result in the development of multi-drug-
resistant (MDR) strains. Tuberculosis in children constitutes a major
problem. There is commercial non availability of the first-line drugs
in pediatric form. Novel antibiotics can be designed to overcome drug
resistance, cut short the duration of the treatment course and to reduce
drug interactions with antiretroviral therapies. A nanotechnology is
one of the most promising approaches for the development of more
effective and compliant medicines. The advancements in nano-
based drug delivery systems for encapsulation and release of anti-TB
drugs can lead to development of a more effective and affordable TB
pharmacotherapy.
The clinical application of nanotechnology in operative
dentistry
Nanotechnologyaimsatthecreationandutilizationofmaterialsand
devices at the atomic, and molecular level, supra molecular structures,
and in the exploitation of unique properties of particles of size 0.1 nm
to 100 nm. Nano filled composite resin materials are believed to offer
excellent wear resistance, strength, and ultimate aesthetics due to their
exceptional polishability and luster retention. In operative dentistry,
nano fillers constitute spherical silicon dioxide (SiO2
) particles with an
average size of 5-40 nm. The real innovation about nano fillers is the
possibility of improving the load of inorganic phase. The effect of this
high filler load is widely recorded in terms of mechanical properties.
Micro hybrid composites with additional load of Nano fillers are the
best choice in operative dentistry. It is expected that in near future,
it would be possible to use a filler material in operative dentistry,
whose shape and composition would closely mimic the optical and
mechanical characteristics of the natural hard tissues (enamel and
dentin). It also explains the basic concepts of fillers in composite resins,
scanning electron microscopy and energy dispersive spectroscopy
evaluation, and filler weight content. Nanocomposite resins are
non-agglomerated discrete nanoparticles that are homogeneously
distributed in resins or coatings to produce nanocomposites have been
successfully manufactured by nano products Corporation. The nano-
filler used is aluminosilicate powder with a mean particle size of 80 ran
1:4 M ratio of alumina to silica and a refractive index of 1.508. These
nano composites have superior hardness, flexural strength, modulus of
elasticity, decreased polymerization shrinkage and also have excellent
handling properties particle size of 80 ran 1:4 M ratio of alumina to
silica and a refractive index of 1.508 [33,34].
Applications in Ophthalmology
The aim of nano medicine is the to monitor, control, construct,
repair, defense, and improve human biological systems at the molecular
level, with the help of nano devices and nanostructures that operate
massively in parallel at the unit cell level, in order to achieve medical
benefit. Principles of nanotechnology are applied to nano medicine
such as bio mimicry and pseudo intelligence. Some applications of
nanotechnology to ophthalmology are include treatment of oxidative
stress; measurement of intraocular pressure; theragnostics; use of nano
particles for treatment of choroidal new vessels, to prevent scars after
glaucoma surgery, and for treatment of retinal degenerative disease
using gene therapy; prosthetics; and regenerative nano medicine. The
current therapeutic challenges in drug delivery, postoperative scarring
will be revolutionized with the help of nanotechnology and will help in
various unsolved problems such as sight-restoring therapy for patients
with retinal degenerative disease [35]. Treatments for ophthalmic
diseases are expected from this emerging field. A novel nanoscale-
dispersed eye ointment (NDEO) for the treatment of severe evaporative
dry eye has been successfully developed [36]. The excipients used as
semisolid lipids were petrolatum and lanolin, as used in conventional
eye ointment, which were coupled with medium-chain triglycerides
(MCT) as a liquid lipid; both phases were then dispersed in polyvinyl
pyrrolidone solution to form nanodispersion. A transmission electron
micrograph showed that the ointment matrix was entrapped in the
nano emulsion of MCT, with a mean particle size of about 100 nm. The
optimized formulation of NDEO was stable when stored for six months
at 4°C, and demonstrated no cytotoxicity to human corneal epithelial
cells when compared with commercial polymer-based artificial tears
(Tears Natural®
Forte). The therapeutic effects of NDEO were evaluated
and demonstrated therapeutic improvement, displaying a trend of
positive correlation with higher concentrations of ointment matrix in
Figure 8: Use of nano particles in Alzheimer's disease.

Med chem
Volume 5(2): 081-089 (2015) - 88
the NDEO formulations compared to a marketed product. Histological
evaluation demonstrated that the NDEO restored the normal corneal
and conjunctival morphology and is safe for ophthalmic application.
Recent research [37] shows applications of various nanoparticulate
systems like microemulsions, nanosuspensions, nanoparticles,
liposomes, niosomes, dendrimers and cyclodextrins in the field of
ocular drug delivery and also depicts how the various upcoming of
nanotechnology like nanodiagnostics, nanoimaging and nanomedicine
can be utilized to explore the frontiers of ocular drug delivery and
therapy.
Surgery
The technique developed by Rice University, two pieces of chicken
meat is fused by a flesh welder, by placing two pieces of chicken
touching each other. In this technique, green liquid containing gold-
coated nano shells is allowed to dribble along the seam and two sides
are weld together. This method can be used arteries which have been
cut during organ transplant. The flesh welder can be used to weld the
artery perfectly [38].
Visualization
Drug distribution and its metabolism can be determined by tracking
movement. Cells were dyed by scientists to track their movement
throughout the body. These dyes excited by light of a certain wavelength
to glow. Luminescent tags were used to dye various numbers of cells.
These tags are quantum dots attached to proteins which penetrate cell
membranes. The dots were of various sizes and bio-inert material. As
a result, sizes are selected so that the frequency of light used to make
a group of quantum dots fluoresce, and used to make another group
incandesce. Thus both groups can be lit with a single light source.
Tissue engineering
In tissue engineering, nanotechnology can be applied to reproduce
or repair damaged tissues. By using suitable nanomaterial-based
scaffolds and growth factors, artificially stimulated cell proliferation, in
organ transplants or artificial implants therapy nano technology can be
useful, which can lead to life extension.
Antibiotic resistance
Antibiotic resistance can be decreased by use of nano particles
in combination therapy. Zinc Oxide nano particles can decrease
the antibiotic resistance and enhance the antibacterial activity of
Ciprofloxacin against microorganism, by interfering with various
proteinsthatareinteractingintheantibioticresistanceorpharmacologic
mechanisms of drugs [39].
Immune response
The nano device bucky balls have been used to alter the allergy/
immune response. They prevent mast cells from releasing histamine
into the blood and tissues, as these bind to free radicals better than any
anti-oxidant available, such as vitamin E [40].
Nano pharmaceuticals
Nano pharmaceuticals can be used to detect diseases at much earlier
stages and the diagnostic applications could build upon conventional
procedures using nanoparticles. Nano pharmaceuticals are an
emerging field where the sizes of the drug particle or a therapeutic
delivery system work at the nanoscale. Delivering the appropriate dose
of a particular active agent to specific disease site still remains difficult
in the pharmaceutical industry. Nano pharmaceuticals have enormous
potential in addressing this failure of traditional therapeutics which
offers site-specific targeting of active agents. Nano pharmaceuticals
can reduce toxic systemic side effects thereby resulting in better patient
compliance.
Pharmaceutical industry faces enormous pressure to deliver high-
quality products to patients while maintaining profitability. Therefore
pharmaceutical companies are using nanotechnology to enhance the
drug formulation and drug target discovery. Nano pharmaceutical
makes the drug discovery process cost effective, resulting in the
improved Research and Development success rate, thereby reducing
the time for both drug discovery and diagnostics.
Application of Nanotechnology in Modified Medicated
Textiles
Using nanotechnology newer antibacterial cotton has been
developed and used for antibacterial textiles. Developmental works
using nanotechnology, new modified antibacterial textiles have been
developed. Application of conventional antimicrobial agents to textiles
has been already reported. This technique has been advanced by a focus
on inorganic nano structured materials that acquire good antibacterial
activity and application of these materials to the textiles [41].
Conclusion
Nano materials have increased surface area and nano scale effects,
hence used as a promising tool for the advancement of drug and gene
delivery, biomedical imaging and diagnostic biosensors. Nano materials
have unique physicochemical and biological properties as compared
to their larger counterparts. The properties of nano materials can
greatly influence their interactions with bio molecules and cells, due
to their peculiar size, shape, chemical composition, surface structure,
charge, solubility and agglomeration. For example, nano particles
can be used to produce exceptional images of tumor sites; single-
walled carbon nanotubes, have been used as high-efficiency delivery
transporters for biomolecules into cells. There is a very bright future
to nano technology, by its merging with other technologies and the
subsequent emergence of complex and innovative hybrid technologies.
Biology-based technologies are intertwined with nanotechnology-
nanotechnology is already used to manipulate genetic material, and
nano materials are already being built using biological components.
The ability of nanotechnology to engineer matter at the smallest scale is
revolutionizing areas such as information technology cognitive science
and biotechnology and is leading to new and interlinking these and
other fields. By further research in nanotechnology, it can be useful for
every aspect of human life. Medicine, regenerative medicine, stem cell
research and nutraceuticals are among the leading sectors that will be
modified by nanotechnology innovations.
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