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OrganoidPresentation
The document discusses organoids, which are 3D clusters of cells grown in vitro to mimic organ structures. It describes the 5 basic steps to make an organoid: 1) obtain a tissue sample, 2) dissociate it into single cells, 3) isolate stem cells using a cell sorter, 4) add the stem cells to a gel-like substance called Matrigel, and 5) allow the cells to grow over 1-90 days. Various types of organoids are listed, and potential uses discussed, including testing medical/chemical agents, aiding drug development by reducing animal testing, and regenerative medicine by growing replacement organs.
OrganoidPresentation
- 1. Organoids Small Cells DoingBig Things Franklin Wilson GTCC North Carolina STEM Alliance
- 2. What is anorganoid? Three-dimensional cluster of cells grown in-vitro
- 3. The making ofan organoid 5 Basic Steps:
- 4. The making ofan organoid 5 Basic Steps: 1. Obtain Sample
- 5. The making ofan organoid 5 Basic Steps: 1. Obtain Sample 2. Mechanically and chemically dissociate tissue into single cells
- 6. The making ofan organoid 5 Basic Steps: 1. Obtain Sample 2. Mechanically and chemically dissociate tissue into single cells 3. Use cell-sorter to obtain target cells (stem cells)
- 7. The making ofan organoid 5 Basic Steps: 1. Obtain Sample 2. Mechanically and chemically dissociate tissue into single cells 3. Use cell-sorter to obtain target cells (stem cells) 4. Add target cells to Matrigel
- 8. The making ofan organoid 5 Basic Steps: 1. Obtain Sample 2. Mechanically and chemically dissociate tissue into single cells 3. Use cell-sorter to obtain target cells (stem cells) 4. Add target cells to Matrigel 5. Allow cells to grow (1-90 days) 0d 4d 6d 12d 14d 22d 8d 25d 11d
- 9. Types of organoids CerebralCortex Hepatic Renal Cardiac Prostatic Gastric Lung Epithelial Intestinal Optic Cup Thyroid Pancreatic Neural Tube Pituitary Mammary
- 10. Potential Uses forOrganoids
- 11. Testing Uses Perform testsof medical, biological, and chemical agents
- 12. Pharmaceutical Uses Benefits todrug manufacturing: • Lower the cost • Decrease time needed • Dramatically reduce the amount of animals testing
- 13. Pharmaceutical Uses Drawbacks: • Nota whole organ • Not a body
- 14. Uses in RegenerativeMedicine
- 15. Uses in RegenerativeMedicine Process hepatic cancer treatment: 1. Excise tumor 2. Dissociate and harvest target cells 3. Use CRISPR to modify DNA 4. Grow cells in Matrigel mold 5. Implant
- 16. Uses in RegenerativeMedicine Benefits to lab grown organs: • Avoid risk of rejection • Decrease transplant list
- 17. Uses in RegenerativeMedicine Drawbacks: • Time needed to grow • Lack of differentiation • Size constraints